NaCl‐induced phosphorylation of light harvesting chlorophyll a/b proteins in thylakoid membranes from the halotolerant green alga, Dunaliella salina

Abstract

Light could induce phosphorylation of light harvesting chlorophyll a/b binding proteins (LHCII) in Dunaliella salina and spinach thylakoid membranes. We found that neither phosphorylation was affected by glycerol, whereas treatment with NaCl significantly enhanced light-induced LHCII phosphorylation in D. salina thylakoid membranes and inhibited that in spinach. Furthermore, even in the absence of light, NaCl and several other salts induced LHCII phosphorylation in D. salina thylakoid membranes, but not in spinach thylakoid membranes. In addition, hypertonic shock induced LHCII phosphorylation in intact D. salina under dark conditions and cells adapted to different NaCl concentrations exhibited similar LHCII phosphorylation levels. Taken together, these results show for the first time that while LHCII phosphorylation of D. salina thylakoid membranes resembles that of spinach thylakoid membranes in terms of light-mediated control, the two differ with respect to NaCl sensitivity under light and dark conditions.

Coordinate expression of light-harvesting chlorophyll a/b gene family of photosystem II and chlorophyll a oxygenase gene regulated by salt-induced phosphorylation in Dunaliella salina

“…The observed parallel high-salinity-induced increase in phosphorylation of LHCII is in agreement with proposed activation of relevant kinases or alteration in the conformation of LHCII (Liu and Shen 2004).…”

“…Liu and Shen (2004) reported that NaCl and several other salts induce LHCII phosphorylation both in vitro or in vivo under dark condition in D. salina but not in spinach. Further evidence indicates that electron inhibitors, 3-(3,4-dichlorophenyl)-1,1-dimethylurea and 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone, which are capable of controlling the redox state of PQ, have no effect on the salt-induced phosphorylation level suggesting that the phosphorylation mechanism induced by salt is different from that induced by light and low pH (Liu and Shen 2005).…”

Gene expression characteristics of growth-inhibited rice seedlings induced by low-energy N+-beam implantation

“…Many homologous genes encoding LHCPs from various plant species belong to one of the 10 gene family members (Teramoto et al, 2001). Under changing light conditions, the reversible phosphorylation of LHCP a/bbinding proteins (LHCII) represents a system for balancing excitation energy between photosystems I and II (Liu and Shen, 2004). The 11 downregulated DEGs (chlorophyll a/b-binding protein) involved in the photosynthesis pathway were less expressed in Samples 2 and 3 than in the control sample ( Table 4.…”

RNA-seq reveals the downregulated proteins related to photosynthesis in growth-inhibited rice seedlings induced by low-energy N+ beam implantation

“…Many homologous genes encoding LHCPs from various plant species belong to 1 of the 10 gene family members (Teramoto et al, 2001). Under changing light conditions, the reversible phosphorylation of LHCP of photosystem II represents a system for balancing excitation energy between photosystems I and II (Liu and Shen, 2004). The 11 down-regulated DEGs involved in a photosynthesis pathway were less expressed in NGI and GI samples than in the control sample.…”