Nachweis von zyklischem Adenosin-3′ : 5′-monophosphat (cAMP) in Primärsprossen von Zea mays L.

Direct evidence has been obtained for the presence of adenosine-3':5'-cyclic monophosphate (cAMP) in tobacco (Nicotiana tabacum L.) callus tissue cultures, bean (Phaseolus vulgaris L.) seedlings and immature kernels of sweet corn (Zea mays L.) through the use of a highly specific and sensitive gas chromatography-mass spectrometric assay. Levels of endogenous cAMP ranged from 70 to 126 pmol/g fresh weight. Corresponding levels of cAMP determined for the same samples using radioimmunoassay were consistently three to four times higher. Contrary to previous reports for citrus plants, measurable levels of cAMP could not be detected in young lemon leaves within the limits of detection of the mass-spectrometric assay method. In the case of tobacco callus tissue, the coumarin glucoside, scopolin, which was present in large amounts and showed similar chromatographic behaviour to cAMP, interferred strongly with the mass-spectrometric measurements of cAMP in inadequately purified extracts. The use of high-performance liquid chromatography, in addition to standard chromatographic purification methods, produced highly purified plant extracts for quantitation of cAMP and also provided a method for the separation of cAMP from its 2':3'-isomer.

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Identification and quantitation of adenosine-3?:5?-cyclic monophosphate in plants using gas chromatography-mass spectrometry and high-performance liquid chromatography

Johnson

MacLeod

Parker

et al. 1981

Planta

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Adenosine 3′,5′-cyclic monophosphate levels in maize roots

Sen

Pilet

1981

Experientia

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The cyclic nucleotide phosphodiesterases of spinach chloroplasts and microsomes

et al. 1980

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Nachweis von zyklischem Adenosin-3′ : 5′-monophosphat (cAMP) in Primärsprossen von Zea mays L.

Cited by 7 publications

References 7 publications

Identification and quantitation of adenosine-3?:5?-cyclic monophosphate in plants using gas chromatography-mass spectrometry and high-performance liquid chromatography

Identification and quantitation of adenosine-3?:5?-cyclic monophosphate in plants using gas chromatography-mass spectrometry and high-performance liquid chromatography

Adenosine 3′,5′-cyclic monophosphate levels in maize roots

The cyclic nucleotide phosphodiesterases of spinach chloroplasts and microsomes

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