Nachweis der spenderorganspezifischen humoralen Antikörper in hyperakut und akut abgestoßenen Lungenallotransplantaten am Hund

Abstract

A method to eluat donororganspecific antibodies enabling direct and specific access to the pattern of humoral rejection after lung allotransplantation is demonstrated. The antibodies were evaluated quantitatively and qualitatively by elution of lung-grafts immunoglobulins and by a consequent investigation of the eluates using the passive haemagglutination- and indirect immunflourescence- test against donors' lung-antigen. Humoral antidonorlung-antibodies could be proved in all rejected grafts belonging to sensitized as well as to unsensitized recipients. There can be seen a highly significant correlation when comparing the results of the passive haemagglutination and indirect immunfluorescence test (r = 0,93, p less than or equal 0,01). On the other hand negative results of the investigations of eluates by lungantigens of other dogs show the specifity of humoral graft rejection. The suprising fact that there does not exist an essential difference between the results of sensitized and nonsensitized animals reveals a humoral immunresponse in hyperacute as well as in acute rejection.

Donor- and Organ-Specific Evaluation of Antibodies Eluted from Canine Lung Allografts Rejected by Immunosuppressively Treated and Untreated Recipients

“…On the contrary, the donor organ specific evaluation of the immunoglobulins by indirect immunofluo rescence revealed no accordance with direct immunofluorescence (table I). Only the lo calization of the immunoglobulins in lung tissue, mainly concerning subendothelial areas of small blood vessels at a granular distribution pattern [13], was found similar with both immunofluorescence techniques Using the remaining recipients' lung sec tions as target for our control studies, none of the eluates revealed positive results in the indirect immunofluorescence assay.…”

“…and there was no accordance with the results of direct immunofluorescence by quantification. The difference in the organ-bound antibodies between immunosuppressively treated and untreated lung grafts seems to be remarkable be cause of a similar mononuclear infiltration.Thus, enabling a specific improvement of some previous speculations about the lungspecific humoral alloimmune reaction, this type of rejection seems to be similar to rather stereotypical allograft rejection, but may be modified by a standard immunosuppression with méthylprednisolone and azathioprine.During the last years, humoral organ transplant rejection not only has won grow ing importance by the discovery of acceler ated rejection [1] and of new humoral im mune factors [12] in general, but especially has been considered to be responsible for certain phenomena in lung allotransplant damage [6,9,20],In a previous study [13] we were able to demonstrate a considerable participation of humoral antibodies in lung allograft rejec tion by DLA-sensitized as well as unsensi tized individuals. Based on such observa tions, the following study is concerned with influences of a standard postoperative im munosuppressive treatment on the develop ment of humoral transplant rejection, which especially might be important for managing the rejection crisis.…”

In vitro Evidence of Cellular and Humoral Immune Responses following Lung Allotransplantation in Canine Recipients with and without Immunosuppressive Treatment

“…IF microscopy was applied according to the method of Coons and Kaplan (4) using FITC-conjugated antisera (IgG fraction, heavy chain specific. Miles Laboratories, Microbiological Association) against canine IgG and IgM as previously reported (9).…”