NAC transcription factor expression, amino acid concentration and growth of elite rice cultivars upon salt stress

Abstract

NAC transcription factors (TF) play important roles in regulating osmotic stress tolerance in plants. We tested the expression of 57 NAC genes in the presence of NaCl in young leaves of two elite rice cultivars, Cotaxtla and Tres Ríos, which display contrasting responses to salinity at the biochemical and physiological levels. Using qRT-PCR, the expression of 41 out of 57 NAC genes was validated, of which 23 showed regulation by NaCl. We identified two NAC genes (Os02g56600 and Os12g07790) induced in Cotaxtla, but repressed in Tres Ríos when plants were exposed to 100 mM NaCl in nutrient solution. In both elite cultivars, treated plants showed higher concentrations of total amino acids and proline in comparison to the controls; in all cases, Cotaxtla plants accumulated more free amino acids and proline than Tres Ríos plants. Furthermore, shoot growth was more affected in both cultivars, while root length was not reduced in treated plants in comparison to the controls. We conclude that both elite rice cultivars exhibit different expression patterns of NAC transcription factors as well as biochemical and physiological responses to salt stress, giving rise to better performance of Cotaxtla plants in comparison to Tres Ríos plants under our experimental conditions.

Aluminum Enhances Growth and Sugar Concentration, Alters Macronutrient Status and Regulates the Expression of NAC Transcription Factors in Rice

“…Primers pairs used in this study were those previously reported by García-Morales et al ( 2014 ) and Caldana et al ( 2007 ). We also tested the expression of the following NAC genes: OsNAC6 (Nakashima et al, 2007 ), OsNAC5 (Sperotto et al, 2009 ), and OsNAC10 (Jeong et al, 2010 ).…”

Expression patterns and promoter analyses of aluminum-responsive NAC genes suggest a possible growth regulation of rice mediated by aluminum, hormones and NAC transcription factors

“…These cultivars were produced for establishment in the tropical soils of Mexico, which display different degrees of acidity. The seeds of these cultivars were disinfected and germinated according to García-Morales et al (2014) [43]. Eleven days after germination, plants were transplanted in containers with 12 L Yoshida nutrient solution which contained 1.43 mM NH 4 NO 3 , 1.00 mM CaCl 2 2H 2 O, 1.64 mM MgSO 4 7H 2 O, 1.30 mM K 2 SO 4 , 0.32 mM NaH 2 PO 4 2H 2 O, 1.00 mM Fe-EDTA, 7.99 μM MnCl 2 4H 2 O, 0.15 μM ZnSO 4 7H 2 O, 0.15 μM CuSO 4 5H 2 O, 0.075 μM (NH 4 ) 6 Mo 7 O 24 4H 2 O, and 1.39 μM H 3 BO 3 .…”

“…Three independent biological replicates were immediately frozen in liquid nitrogen and stored at -80 ºC. RNA extraction, cDNA synthesis, and qRT-PCR were carried out as described by García-Morales et al (2014) [43] and Moreno-Alvarado et al (2017) [1]. The primers for the NAC transcription factors were those previously used by García-Morales et al (2014) [43] and selected from those reported by Caldana et al (2007) [44].…”

“…RNA extraction, cDNA synthesis, and qRT-PCR were carried out as described by García-Morales et al (2014) [43] and Moreno-Alvarado et al (2017) [1]. The primers for the NAC transcription factors were those previously used by García-Morales et al (2014) [43] and selected from those reported by Caldana et al (2007) [44]. In this study we also included the genes OsNAC6 [45], OsNAC5 [46], and OsNAC10 [47].…”

Overexpression of PaNAC03, a stress induced NAC gene family transcription factor in Norway spruce leads to reduced flavonol biosynthesis and aberrant embryo development

“…Interestingly, the biotic stress responsive gene models to (PaNAC03, PaNAC04, PaNAC05 and MA_86256p0010) cluster together in the phylogeny with MA_103386p0010 and MA_64687p0010, which do not respond to H. annosum s.l. inoculation, forming a sister group to the Arabidopsis ANAC032, ATAF1, ATAF2 and ANAC102 proteins and to a group of rice NAC genes known to respond to abiotic stress [58]. This differentiation in terms of expression pattern between Norway spruce paralogs is consistent with the concept of subfunctionalization [59].…”