NAADP activates a Ca<sup>2+</sup>current that is dependent on F‐actin cytoskeleton

Moccia, Francesco; Lim, Dmitri; Nusco, Gilda A.; Ercolano, Emanuela; Santella, Luigia

doi:10.1096/fj.03-0178fje

Cited by 64 publications

(53 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There has even been speculation about a physical link between lysosomally located NAAPD ϩ receptors and Ca 2ϩ channels in the plasma membrane (45) blocker. Similarly, in starfish oocytes, the Ca 2ϩ response was sensitive to thapsigargin (25), and thapsigargin also abolished the NAADP ϩ -stimulated secretion of insulin in human ␤-cells (34). The fact that no massive Ca 2ϩ release from the endoplasmic reticulum was detected after lysosomal disruption could be explained by the hypothesis that lysosomal activation by NAADP ϩ is a requirement for the subsequent activation of Ca 2ϩ release from thapsigargin-sensitive stores.…”

Section: Discussionmentioning

confidence: 93%

“…Also in mature starfish oocytes, the amplitude of the response to NAADP ϩ could be strongly reduced by the L-type Ca 2ϩ channel blockers verapamil and nifedipine (27). Moreover, NAADP ϩ induced a Ca 2ϩ influx, as measured by the patch clamp technique, which could be significantly reduced by L-type Ca 2ϩ channel blockers (25). There has even been speculation about a physical link between lysosomally located NAAPD ϩ receptors and Ca 2ϩ channels in the plasma membrane (45) blocker.…”

Section: Discussionmentioning

confidence: 98%

“…In intact cells, both types of stores interact, and it was suggested that the NAADP ϩ -activated stores serve to prime the other stores and are involved in initiating Ca 2ϩ oscillations then maintained by IP 3 and cADPR (24). In addition, there is evidence for Ca 2ϩ influx in response to NAADP ϩ (25).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Extracellular Application of Nicotinic Acid Adenine Dinucleotide Phosphate Induces Ca2+ Signaling in Astrocytes in Situ

Heidemann

Schipke

Kettenmann

2005

Journal of Biological Chemistry

View full text Add to dashboard Cite

Astrocytes are in intimate contact with neurons, in particular with synapses, and are able to sense, react to, and even influence neuronal activity. The astrocytic response to neuronal activity can be most readily detected by observing changes in the intracellular Ca 2ϩ concentration mediated via transmitter receptors (e.g. glutamate, GABA (␥-aminobutyric acid), or acetylcholine) or via other messengers, such as nitric oxide. Ca 2ϩ increases can occur by Ca 2ϩ influx via the plasma membrane as found for the nitric oxide-mediated neuron-glia signaling in the cerebellum (1). Ca 2ϩ can furthermore be released from intracellular stores by activation of metabotropic receptors such as GABA B receptors, which mediate neuron-glia signaling in the hippocampus (2). Indeed, astrocytes express a variety of receptors linked to the Ca 2ϩ mobilization from intracellular stores (3). To produce specific signals with one and the same messenger (Ca 2ϩ ), several second messenger pathways and different sources of Ca 2ϩ are involved. It seems important, at the current stage, to decipher the astrocytic Ca 2ϩ code, since this would lead to further understanding of how these cells can react to and influence the neuronal network.The best investigated second messenger linking activation of metabotropic receptors and Ca 2ϩ release is inositol 1,4,5-trisphosphate (IP 3 ). 2The importance of other intracellular messengers, such as cyclic ADPribose (cADPR), is less well understood (4) or still hypothetical as for the two sphingomyelin metabolites sphingosine 1-phosphate (5) and sphingosylphosphorylcholine (6). Almost a decade ago, a novel intracellular Ca 2ϩ -releasing second messenger was identified in sea urchin eggs, nicotinic acid adenine dinucleotide phosphate (NAADP ؉ ) (7), which binds to an unknown receptor. Since its discovery, there is increasing evidence that NAADP ϩ also has a physiological role in Ca 2ϩ signal transduction in vertebrates, including mammalian cells (8). In the vertebrate nervous system, intracellular Ca 2ϩ release by NAADP ϩ has only been demonstrated from rat brain microsome preparations (9) and in frog motoneurons (10). NAADP ϩ -specific binding sites are found in gray and white matter of rat brain, but the cellular specificity of the binding site has not yet been determined (11). The major way of NAADP ϩ synthesis in mammalian tissue takes place under acidic conditions and in the presence of nicotinic acid from NADP ϩ by the type II transmembrane glycoprotein ADP-ribosyl cyclase CD38. In a neutral or alkaline environment, CD38 exclusively catalyzes the conversion of NAD ϩ into cADPR (12). Apart from cell membrane localization, CD38 has been located to various intracellular organelle membranes and can moreover be internalized by endocytosis (13). Therefore, NAADP ϩ synthesis most likely takes place in membranes of intracellular acidic organelles such as lysosomes or late endosomes, whereas CD38 in the plasma membrane synthesizes cADPR. Regarding the mammalian central nervous system, CD38 expression was demonstr...

show abstract

Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 98%

See 1 more Smart Citation

Extracellular Application of Nicotinic Acid Adenine Dinucleotide Phosphate Induces Ca2+ Signaling in Astrocytes in Situ

Heidemann

Schipke

Kettenmann

2005

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…Again, similar to the sea urchin egg, imaging of the Ca 2+ increase in response to NAADP reveals that NAADP-induced Ca 2+ release initiates in the cortex of matured oocyte (Lim et al, 2001). An inwardly rectifying Ca 2+ -selective membrane current activated by NAADP has recently been characterised (Moccia et al, 2003). This current is insensitive to a combination of heparin and 8-NH 2 -cADPR which (as discussed above) is sufficient to attenuate global Ca 2+ increases in response to NAADP (Santella et al, 2000).…”

Section: +mentioning

confidence: 98%

NAADP‐induced Ca²⁺ Release — A new signalling pathway

Patel

2004

Biology of the Cell

View full text Add to dashboard Cite

Changes in cystosolic Ca2+ concentration are critical for the regulation of numerous cellular events. Mobilisation of intracellular Ca 2+ stores by Ca 2+ mobilising messengers is a highly conserved mechanism whereby different agonists mediate their cellular effects. NAADP is the newest of these messengers to be described. Accumulating evidence suggests that NAADP targets a novel intracellular Ca 2+ release channel that under certain conditions can inactivate prior to opening. These channels are likely located on Ca 2+ stores distinct from the endoplasmic reticulum, the activation of which evokes complex changes in Ca 2+ involving cross-talk with other intracellular Ca 2+ channels. Recent demonstrations of changes in cellular NAADP levels by physiologically relevant stimuli establish the importance of this molecule in the control of calcium dynamics.

show abstract

“…Hence, in starfish eggs, NAADP appeared to work on Ca 2+ channels at the plasma membrane [91]. NAADP also triggered the sperm-evoked initial depolarization (fertilization potential) in starfish eggs by the activation of a Ca 2+ -mediated inward current, which is mainly carried by Ca 2+ and displays an inwardly-rectifying I-V relationship [101,102]. Taken together, these results suggested that NAADP could recapitulate some of the early events induced by the fertilizing sperm.…”

Section: Roles Of Naadp and Insp 3 For The Ca 2+ Response At Fertilizmentioning

confidence: 99%

Actin, more than just a housekeeping protein at the scene of fertilization

Santella

Chun

2011

Sci. China Life Sci.

Self Cite

View full text Add to dashboard Cite

Since the first demonstration of sperm entry into the fertilized eggs of Mediterranean sea urchin Paracentrotus lividus by Hertwig (1876), enormous progress and insights have been made on this topic. However, the precise molecular mechanisms underlying fertilization are largely unknown. The two most dramatic changes taking place in the zygote immediately after fertilization are: (i) a sharp increase of intracellular Ca 2+ that initiates at the sperm interaction site and traverses the egg cytoplasm as a wave, and (ii) the concomitant dynamic rearrangement of the actin cytoskeleton. Traditionally, this has been studied most extensively in the sea urchin eggs, but another echinoderm, starfish, whose eggs are much bigger and transparent, has facilitated experimental approaches using microinjection and fluorescent imaging methodologies. Thus in starfish, it has been shown that the sperm-induced Ca 2+ increase in the fertilized egg can be recapitulated by several Ca 2+ -evoking second messengers, namely inositol 1,4,5-trisphosphate (InsP 3 ), cyclic ADP-ribose (cADPr) and nicotinic acid adenine dinucleotide phosphate (NAADP), which may play distinct roles in the generation and propagation of the Ca 2+ waves. Interestingly, it has also been found that the dynamic rearrangement of the actin cytoskeleton in the fertilized eggs plays pivotal roles in guiding monospermic sperm entry and in the fine modulation of the intracellular Ca 2+ signaling. As it is well known that Ca 2+ regulates the structure of the actin cytoskeleton, our finding that Ca 2+ signaling can be reciprocally affected by the state of the actin cytoskeleton raises an intriguing possibility that actin and Ca 2+ signaling may form a 'positive feedback loop' that accelerates the downstream events of fertilization. Perturbation of the cortical actin networks also inhibits cortical granules exocytosis. Polymerizing actin bundles also compose the 'acrosome process,' a tubular structure protruding from the head of fertilizing sperm. Hence, actin, which is one of the most strictly conserved proteins in eukaryotes, modulates almost all major aspects of fertilization. starfish, calcium, egg maturation, fertilization, PIP2, actin cytoskeleton, cofilin Citation:Santella L, Chun J T. Actin, more than just a housekeeping protein at the scene of fertilization.

show abstract

NAADP activates a Ca²⁺current that is dependent on F‐actin cytoskeleton

Cited by 64 publications

References 48 publications

Extracellular Application of Nicotinic Acid Adenine Dinucleotide Phosphate Induces Ca2+ Signaling in Astrocytes in Situ

Extracellular Application of Nicotinic Acid Adenine Dinucleotide Phosphate Induces Ca2+ Signaling in Astrocytes in Situ

NAADP‐induced Ca²⁺ Release — A new signalling pathway

Actin, more than just a housekeeping protein at the scene of fertilization

Contact Info

Product

Resources

About

NAADP activates a Ca2+current that is dependent on F‐actin cytoskeleton

Cited by 64 publications

References 48 publications

Extracellular Application of Nicotinic Acid Adenine Dinucleotide Phosphate Induces Ca2+ Signaling in Astrocytes in Situ

Extracellular Application of Nicotinic Acid Adenine Dinucleotide Phosphate Induces Ca2+ Signaling in Astrocytes in Situ

NAADP‐induced Ca2+ Release — A new signalling pathway

Actin, more than just a housekeeping protein at the scene of fertilization

Contact Info

Product

Resources

About

NAADP activates a Ca²⁺current that is dependent on F‐actin cytoskeleton

NAADP‐induced Ca²⁺ Release — A new signalling pathway