NAA at a high concentration promotes efficient plant regeneration via direct somatic embryogenesis and SE-mediated transformation system in Ranunculus sceleratus

Xu, K.-D.; Wang, Wei; Yu, De shui; Li, Xiao li; Chen, Jia min; Feng, Bo; Zhao, Ya wen; Cheng, Meng jia; Liu, Xin xin; Li, Cheng wei

doi:10.1038/s41598-019-54538-8

Cited by 12 publications

(7 citation statements)

References 27 publications

(35 reference statements)

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“…The dark conditions promote somatic embryo formation and development of roots in in A. violaceum. Similar results were reported by Xu Kd [28] in Ranunculus sceleratus, where darkness enhanced the frequency of somatic embryo formation. Cold storage can enhance somatic embryo conversion frequency, potentially caused by epigenetic changes triggered by temperature stress [46].…”

Section: Discussionsupporting

confidence: 90%

“…The seeds of A. violaceum Jacq. ex Stapf were collected in the month of August (10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28) as soon as the follicle burst for seed dispersal, from the villages of Khawous and Numsuru (3220 m asl to 3340 m asl; 34 • 15.905 N to 34 • 12.705 N; 75 • 96.040 E to 75 • 96.720 E) in Ladakh, India. The plant specimen was identified and authenticated by the Center for Biodiversity and Taxonomy (CBT), Department of Botany, University of Kashmir, Hazratbal-Srinagar.…”

Section: Collection Of Plant Materialsmentioning

confidence: 99%

“…Hatano et al [27] regenerated complete plantlets from the somatic embryo of anther explant in Aconitum carmichaeli. High concentrations of NAA (10 mg L −1 ) and darkness promote somatic embryo development in Ranunculus sceleratus [28]. After surveying extensive literature from various databases (DOAJ, Google Scholar, PubMed, Scihub, ScienceDirect, etc.)…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Propagation of Aconitum violaceum Jacq. ex Stapf through Seed Culture and Somatic Embryogenesis

et al. 2022

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Aconitum violaceum Jacq. ex Stapf is a threatened medicinal plant with restricted global distribution. The highest frequency of seed germination was recorded on Murashige and Skoog’s (MS) basal medium, supplemented with 0.5 mg L−1 kinetin with a germination rate of 77.32% and mean germination time of 27 days. Among the various plant growth regulators examined, 0.1 mg L−1 kinetin (Kn) + 0.5 mg L−1 indole-3-acetic acid (IAA) proved to be effective for maximum embryogenic callus production (51.0%) within 31 days of inoculation. The conversion rate of somatic embryos into complete plantlets was highest in the MS medium augmented with 0.1 mg L−1 Kn + 0.5 mg L−1 IAA (68.00%), with an average root initiation time of 25 days. The rooted plantlets were subsequently hardened into jiffy pots with a combination of loamy soil, coco-peat, and vermicompost (1:1:1 v/v), and then transplanted into a greenhouse with a 60% survival rate. To our knowledge, this is the first study on direct in vitro propagation and embryogenic callus induction from seeds. The established regeneration protocol could be employed to propagate A. violaceum on a large scale in a short time. This would contribute significantly to its rapid propagation and germplasm conservation, and establish a framework for the domestication of this highly valued threatened medicinal plant.

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Section: Discussionsupporting

confidence: 90%

Section: Collection Of Plant Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In Vitro Propagation of Aconitum violaceum Jacq. ex Stapf through Seed Culture and Somatic Embryogenesis

et al. 2022

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“…Somatic embryogenesis is a powerful biotechnological tool that can be exploited for various applications, including clonal propagation, plant improvement and germplasm conservation; it also offers an excellent system to study embryo and early plant development, morphogenesis, and genetic transformation (Zimmerman 1993;Hutchinson et al 1996;Demeter et al 2010;Gerdakaneh et al 2011). Among the examples of genera from the family Ranunculaceae in which somatic embryogenesis has been employed, there are, for instance, Aconitum (Giri et al 1993), Actaea (Pinker and Wernicke 2017; Pinker and Schenk 2018), Clematis (Mandegaran and Sieber 2000), Hepatica (Szewczyk-Taranek and Pawłowska 2015), Pulsatilla (Lin et al 2011), and Ranunculus (Beruto and Debergh 1992;Xu et al 2019). Only a few published reports concern somatic embryogenesis for N. sativa (Elhag et al 2004) and N. damascena (Raman and Greyson 1974;Sethi and Rangaswamy 1976), in which the culture media were supplemented with NAA or kinetin.…”

Section: Discussionmentioning

confidence: 99%

Effective callus induction and plant regeneration in callus and protoplast cultures of Nigella damascena L.

Klimek-Chodacka

Kadluczka

Łukasiewicz

et al. 2020

Plant Cell Tiss Organ Cult

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In this study we report the development of effective in vitro systems for a medicinal plant Nigella damascena L. comprising: (1) callus induction, (2) somatic embryogenesis in callus cultures with subsequent plant regeneration, and (3) isolation and regeneration of callus-derived protoplasts. Callus development was achieved on 83–100% of hypocotyl and cotyledon explants, whereby Murashige and Skoog medium (MS) supplemented with 3 mg L−1 6-benzylaminopurine and 0.5 mg L−1α-naphthaleneacetic acid (NAA; BN medium) was more advantageous than MS with kinetin and NAA (KN medium). Histological observations of calli revealed the presence of embryogenic zones from which somatic embryos developed on the hormone-free medium. Plant regeneration was observed on 76–95% of calli. A high capacity to form somatic embryos and regeneration was maintained in long-lasting cultures, i.e. even in 2 year old callus. The obtained callus was also a good source tissue for protoplast isolation. By applying a mixture of cellulase and pectolyase, the acceptable yield of viable protoplasts was achieved, especially from hypocotyl-derived callus maintained on BN medium. Protoplasts embedded in an alginate matrix and cultured in modified Kao and Michayluk media re-constructed their cell wall and re-entered mitotic divisions. About 30% of small cell aggregates formed microcalli, which, after the release from alginate, proliferated continuously on KN and BN media, irrespective of the tissue variant used as the protoplast source. Somatic embryo formation and plant regeneration were successful on hormone-free media. An effective plant regeneration system of N. damascena protoplast cultures has been developed and is being reported for the first time.

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“…There is a lot of scientific data from animal models of epilepsy (especially after SE induction) demonstrating a significant decrease in NAA levels [41,46,47]. The NAA level reflects the quantity and functional metabolic state of neurons, and its concentration decreases with neuronal damage and/or dysfunction [48,49]. Moreover, Campos et al [50] studying TLE patients showed decreased NAA concentrations.…”

Section: Discussionmentioning

confidence: 99%

Effect of Lacosamide and Ethosuximide Chronic Treatment on Neural Precursor Cells and Cognitive Functions after Pilocarpine Induced Status Epilepticus in Mice

et al. 2021

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Seizures in about 40% of patients with epilepsy fail to respond to anti-seizure medication (ASM) and may lead to uncontrolled and prolonged seizures often inducing status epilepticus (SE). The aim of the study was to evaluate the impact of a long-term treatment with two different generation ASMs: ethosuximide (ETS, a classic ASM) and lacosamide (LCM, a 3rd generation ASM) on neural stem cells’ (NSCs’) proliferation and learning and memory functions after pilocarpine (PILO)-induced SE in mice. The following drugs were used: LCM (10 mg/kg), ETS (20 mg/kg), and PILO (300 mg/kg). Cell counting was done using confocal microscope and ImageJ software. Cognitive functions were evaluated with the Morris water maze (MWM) test. The level of several selected neurometabolites was measured with magnetic resonance spectroscopy (MRS). Obtained results indicated no significant impact of ETS treatment on the neurogenesis process in PILO mice. Interestingly, LCM significantly decreased the total amount of newborn neurons. The MWM test indicated no significant changes in the time and distance traveled by the ETS and LCM groups compared to PILO control mice, although all measured parameters were more favorable for the PILO mice treated with ASM. Conclusions: The presented results show that long term treatment with LCM and ETS seems to be safe for the cognitive functions and the proper course of neurogenesis in the mouse PILO-induced SE model, although one should remember that LCM administered chronically may act to reduce new neurons’ formation.

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NAA at a high concentration promotes efficient plant regeneration via direct somatic embryogenesis and SE-mediated transformation system in Ranunculus sceleratus

Cited by 12 publications

References 27 publications

In Vitro Propagation of Aconitum violaceum Jacq. ex Stapf through Seed Culture and Somatic Embryogenesis

In Vitro Propagation of Aconitum violaceum Jacq. ex Stapf through Seed Culture and Somatic Embryogenesis

Effective callus induction and plant regeneration in callus and protoplast cultures of Nigella damascena L.

Effect of Lacosamide and Ethosuximide Chronic Treatment on Neural Precursor Cells and Cognitive Functions after Pilocarpine Induced Status Epilepticus in Mice

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