Na+/H+ antiporters are differentially regulated in response to NaCl stress in leaves and roots of Mesembryanthemum crystallinum

Cosentino, Cristian; Fischer-Schliebs, Elke; Bertl, Adam; Thiel, Gerhard; Homann, Ulrike

doi:10.1111/j.1469-8137.2010.03208.x

Cited by 62 publications

(55 citation statements)

References 43 publications

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“…Strikingly, in some cases, salinity produced little or even no alteration of SOS1 transcription (Cosentino et al 2010;Kant et al 2006;Mullan et al 2007;Taji et al 2004;Wu et al 2007). By contrast, in other plants the salt challenge induced a clear accumulation of SOS1 mRNA (Martinez-Atienza et al 2007;Olías et al 2009;Wang et al 2010;Tang et al 2010;Xu et al 2008).…”

Section: Expression Pattern Of Tdsos1 Under Different Stress Conditionsmentioning

confidence: 82%

Regulation of durum wheat Na+/H+ exchanger TdSOS1 by phosphorylation

et al. 2011

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We have identified a plasma membrane Na(+)/H(+) exchanger from durum wheat, designated TdSOS1. Heterologous expression of TdSOS1 in a yeast strain lacking endogenous Na(+) efflux proteins showed complementation of the Na(+)- and Li(+)-sensitive phenotype by a mechanism involving cation efflux. Salt tolerance conferred by TdSOS1 was maximal when co-expressed with the Arabidopsis protein kinase complex SOS2/SOS3. In vitro phosphorylation of TdSOS1 with a hyperactive form of the Arabidopsis SOS2 kinase (T/DSOS2∆308) showed the importance of two essential serine residues at the C-terminal hydrophilic tail (S1126, S1128). Mutation of these two serine residues to alanine decreased the phosphorylation of TdSOS1 by T/DSOS2∆308 and prevented the activation of TdSOS1. In addition, deletion of the C-terminal domain of TdSOS1 encompassing serine residues at position 1126 and 1128 generated a hyperactive form that had maximal sodium exclusion activity independent from the regulatory SOS2/SOS3 complex. These results are consistent with the presence of an auto-inhibitory domain at the C-terminus of TdSOS1 that mediates the activation of TdSOS1 by the protein kinase SOS2. Expression of TdSOS1 mRNA in young seedlings of the durum wheat variety Om Rabia3, using different abiotic stresses (ionic and oxidative stress) at different times of exposure, was monitored by RT-PCR.

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Section: Expression Pattern Of Tdsos1 Under Different Stress Conditionsmentioning

confidence: 82%

Regulation of durum wheat Na+/H+ exchanger TdSOS1 by phosphorylation

et al. 2011

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“…Consequently, other transporters, in addition to and different from NHX, are likely to be involved in vacuolar Na + sequestration. In addition, salt-induced up-regulation of Na + /H + antiporter expression levels has been observed in leaves but not in roots (Cosentino et al, 2010), suggesting the importance of Na + exclusion and intracellular sequestration, primarily in photosynthesizing cells. Thus, tissue-and speciesspecific differences in the respective mechanisms should be considered as well.…”

mentioning

confidence: 99%

Reduced Tonoplast Fast-Activating and Slow-Activating Channel Activity Is Essential for Conferring Salinity Tolerance in a Facultative Halophyte, Quinoa

et al. 2013

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Halophyte species implement a "salt-including" strategy, sequestering significant amounts of Na + to cell vacuoles. This requires a reduction of passive Na + leak from the vacuole. In this work, we used quinoa (Chenopodium quinoa) to investigate the ability of halophytes to regulate Na + -permeable slow-activating (SV) and fast-activating (FV) tonoplast channels, linking it with Na + accumulation in mesophyll cells and salt bladders as well as leaf photosynthetic efficiency under salt stress. Our data indicate that young leaves rely on Na + exclusion to salt bladders, whereas old ones, possessing far fewer salt bladders, depend almost exclusively on Na + sequestration to mesophyll vacuoles. Moreover, although old leaves accumulate more Na + , this does not compromise their leaf photochemistry. FV and SV channels are slightly more permeable for K + than for Na + , and vacuoles in young leaves express less FV current and with a density unchanged in plants subjected to high (400 mM NaCl) salinity. In old leaves, with an intrinsically lower density of the FV current, FV channel density decreases about 2-fold in plants grown under high salinity. In contrast, intrinsic activity of SV channels in vacuoles from young leaves is unchanged under salt stress. In vacuoles of old leaves, however, it is 2-and 7-fold lower in older compared with young leaves in control-and saltgrown plants, respectively. We conclude that the negative control of SV and FV tonoplast channel activity in old leaves reduces Na + leak, thus enabling efficient sequestration of Na + to their vacuoles. This enables optimal photosynthetic performance, conferring salinity tolerance in quinoa species.The increasing problem of global land salinization (Flowers, 2004;Rengasamy, 2006) and its associated multibillion dollar losses in agricultural production require a better understanding of the key physiological mechanisms that confer salinity tolerance in crops. One effective way of gaining such knowledge comes from studying halophytes (Glenn et al., 1999;Flowers and Colmer, 2008;Shabala and Mackay, 2011).One of the prominent features of halophytes is their ability to efficiently sequester cytosolically toxic Na + to the cell vacuole. The classic view is that this sequestration is achieved by tonoplast Na + /H + antiporters (Barkla et al., 1995;Flowers and Colmer, 2008), a process energized by both vacuolar H + pumps: ATPase (Ayala et al., 1996;Vera-Estrella et al., 1999;Wang et al., 2001) and pyrophosphatase (Parks et al., 2002;Vera-Estrella et al., 2005;Guo et al., 2006;Krebs et al., 2010). However, recent studies have added more complexity to the relationship between Na + /H + antiporters and vacuolar Na + sequestration, assigning a role to the transporter in the regulation of K + and H + homeostasis (for review, see Rodríguez-Rosales et al., 2009;Jiang et al., 2010; Bassil et al., 2011 (Yamaguchi et al., 2005). Consequently, other transporters, in addition to and different from NHX, are likely to be involved in vacuolar Na + sequestration. In addition...

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“…In order to obtain the full-length cDNA sequence of ASIP from Chinese raccoon dog, 5' and 3' rapid-amplification of cDNA ends (RACE) was carried out according to the protocol reported by Cosentino et al (2010). Gene-specific primers were designed based on the canine ASIP mRNA sequence (GenBank accession No.…”

Section: Methodsmentioning

confidence: 99%

Cloning and identification of the ASIP gene in Chinese raccoon dog (Nyctereutes procyonoides procyonoides)

Guo

et al. 2015

Genet. Mol. Res.

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ABSTRACT.The quantity, quality, and distribution of eumelanin and pheomelanin determine a wide variety of coat colors in animals. Three coat color variants exist in farmed wild-type Chinese raccoon dog (Nyctereutes procyonoides procyonoides), which is an important fur-bearing animal species. The ASIP gene is an important candidate gene for coat color variation in some species. In this study, the complete cDNA sequences of ASIP were amplified from a wild-type Chinese raccoon dog. Sequence analysis revealed the coding region of ASIP in Chinese raccoon dog to be 396-bp in length and two transcripts (accession Nos. KT224450 and KT224451) were identified due to the alternative use of exon 1 (1A and 1C). However, the alternative splicing pattern and the coding sequence of ASIP in three types of coat color variants were the same as those identified in the wild-type individual. Based on the results obtained in this study, we can exclude a role for alternative splicing of exon 1 and the coding sequence of ASIP in coat color variation in Chinese raccoon dog.

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Na⁺/H⁺ antiporters are differentially regulated in response to NaCl stress in leaves and roots of Mesembryanthemum crystallinum

Abstract: Summary• Salinity tolerance in plants involves controlled Na + transport at the site of Na + accumulation and intracellular Na + compartmentation. McNhaD in chloroplasts and demonstrates that the chloroplast is one of the compartments involved in the response of cells to salt stress.

Cited by 62 publications

References 43 publications

Regulation of durum wheat Na+/H+ exchanger TdSOS1 by phosphorylation

Regulation of durum wheat Na+/H+ exchanger TdSOS1 by phosphorylation

Reduced Tonoplast Fast-Activating and Slow-Activating Channel Activity Is Essential for Conferring Salinity Tolerance in a Facultative Halophyte, Quinoa

Cloning and identification of the ASIP gene in Chinese raccoon dog (Nyctereutes procyonoides procyonoides)

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