Na+ pump current‐voltage relationships of rabbit cardiac Purkinje cells in Na(+)‐free solution.

Abstract

SUMMARY1. The Na+ pump current (Ip) of isolated, single rabbit cardiac Purkinje cells in Na+-free solution was measured at 32-34 'C by means of whole-cell recording.2. The Ip amplitude was studied as a function of clamp potential (V,)

The antagonistic effect of K+o and dihydro‐ouabain on the Na+ pump current of single rat and guinea‐pig cardiac cells.

“…Similarly, Ip activation by K+ is facilitated in cardiac ventricular mnyocytes (Nakao & Gadsby, 1989) and Purkinje cells (Bielen, Glitsch & Verdonck, 1993) superfused with Na+-free solution. Since the results presented so far revealed that activation of the Na+-K+ pUInp by K+-binding affects the interaction between DHO and the pumimp, we thought it miglht be interesting to studly the possiible effect of Na+ on the DHO-K+ Figs 1A, B, and 2A but Na+-free, choline-containing superfusion mnedia wAere applied to cells as well.…”

Change of Na+ pump current reversal potential in sheep cardiac Purkinje cells with varying free energy of ATP hydrolysis.

“…KSmall differences in the shape of the p-V relationships may be due to the different concentrations of the external pump activator cation used (cf. Bielen, Glitsch & Verdonck, 1993).…”

“…However, it is helpful to realize that the mean values of the measured I,p are not significantly different from zero between -20 and -95 mV (Fig. 5) (Rakowski, Vasilets, La Tona & Schwarz, 1991;Omay & Schwarz, 1992) and freshly isolated rabbit cardiac Purkinje cells (Bielen et al 1993 …”

The effect of cardiac glycosides on the Na+ pump current‐voltage relationship of isolated rat and guinea‐pig heart cells.

“…Figure 6D shows the exponential time course of the slow component. Bielen et al (1991aBielen et al ( , 1993 Fig. 4B and D).…”