(Na+K+)-stimulated adenosinetriphosphatase in isolated intestinal villus tip and crypt cells

Charney, Alan N.; Gots, Ronald E.; Giannella, Ralph A.

doi:10.1016/0005-2736(74)90083-2

Cited by 45 publications

(18 citation statements)

References 23 publications

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“…4). The specific activities of Mg-ATPase and adenylate cyclase Intestinal Na-K-ATPase and Electrolyte Transport after Steroids Na-K-ATPase activity (20,26) and sodium and water absorption (27). When ileal transport and enzyme activity were measured 24 h after a single injection of methylprednisolone 3 mg/100 g, similar increases in sodium and water absorption, potassium secretion, and Na-K-ATPase activity (59.3±2.7 (4) vs. 35.0±1.7 (6) icmol Pi/mg protein per h, P < 0.0005) were found.…”

Section: Resultsmentioning

confidence: 99%

“…METHODS Normal male albino Walter Reed rats' weighing 200-300 g were maintained on a standard diet with free access to water. Methylprednisolone acetate (Depo-Medrol, Upjohn Co., Kalamazoo, Mich.) was injected subcutaneously as an aqueous suspension in a dose of 3 mg/100 g per day for 1 or (21), thymidine kinase by the method of Breitman (22), and ATPase activity (20).…”

Section: Introductionmentioning

confidence: 99%

“…Details of the pellet preparation, the distribution of ATPase activities among the fractions obtained by centrifugation, and the ATPase assay have been described (20,23). Results were expressed as micromoles of inorganic phosphate (Pi) liberated per millgram protein per hour.…”

Section: Introductionmentioning

confidence: 99%

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Na+-K+-activated adenosine triphosphatase and intestinal electrolyte transport. Effect of adrenal steroids.

Charney¹,

Kinsey²,

Myers³

et al. 1975

J. Clin. Invest.

169

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A B S T R A C T Sodium-potassium-activated adenosine triphosphatase (Na-K-ATPase) is associated with electrolyte transport in many tissues. To help delineate its role in intestinal transport, changes in rat intestinal electrolyte and water transport induced by injecting methylprednisolone acetate 3 mg/100 g or deoxycorticosterone acetate (DOCA) 0.5 mg/100 g per day for 3 days were correlated with changes in Na-K-ATPase activity. Methylprednisolone increased sodium and water absorption, potassium secretion, transmural potential difference, and Na-K-ATPase activity in the jejunum, ileum, and colon. Examination of isolated epithelial cells demonstrated that the jejunal and ileal increase in Na-K-ATPase occurred in both the villus tip and crypt areas. The time-courses of the ileal enzyme and transport changes were identical. Permeability, Mg-ATPase, and adenylate cyclase activities were unchanged by methylprednisolone. DOCA increased sodium and water absorption, potassium secretion, transmural potential difference, and Na-K-ATPase activity in the colon alone. Colonic Mg-ATPase and adenylate cyclase activities were unaffected. Jejunal and ileal enzyme activity, electrolyte transport, and permeability were unchanged by DOCA. Methylprednisolone and DOCA were not additive in their effect on colonic Na-K-ATPase activity. Methylprednisolone and DOCA increased electrolyte and water transport and Na-K-ATPase activity concomitantly in specific segments of small intestine and colon. These data are consistent with an important role for Na-K-ATPase in intestinal electrolyte and water transport.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Na+-K+-activated adenosine triphosphatase and intestinal electrolyte transport. Effect of adrenal steroids.

Charney¹,

Kinsey²,

Myers³

et al. 1975

J. Clin. Invest.

169

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show abstract

“…The cell extrudes sodium from basolateral membrane into the plasma by active transport [24,26]. The specific activity of Na-K-ATPase is higher (three times higher in the jejunum and two times higher in the ileum) in the villus tip than in the crypt cells of the intestine [27].…”

Section: Discussionmentioning

confidence: 99%

Effect of Rejection on Electrophysiologic Function of Canine Intestinal Grafts: Correlation with Histopathology and Na-K-ATPase Activity

Takeyoshi

Zhang

Kokudo

et al. 1995

Journal of Investigative Surgery

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“…Fed white male New Zealand rabbits (2-2.5 kg [9]. 150 mg tissue was homogenized in 7.5 ml 5 mM MgC12, 10% sucrose, 50 mM Tris-HC1 (pH 7.5) using a ground-glass hand homogenizer.…”

Section: Methodsmentioning

confidence: 99%

Calcium and calmodulin‐dependent protein phosphorylation in rabbit ileum

et al. 1981

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(Na+K+)-stimulated adenosinetriphosphatase in isolated intestinal villus tip and crypt cells

Cited by 45 publications

References 23 publications

Na+-K+-activated adenosine triphosphatase and intestinal electrolyte transport. Effect of adrenal steroids.

Na+-K+-activated adenosine triphosphatase and intestinal electrolyte transport. Effect of adrenal steroids.

Effect of Rejection on Electrophysiologic Function of Canine Intestinal Grafts: Correlation with Histopathology and Na-K-ATPase Activity

Calcium and calmodulin‐dependent protein phosphorylation in rabbit ileum

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