Na+, K+-ATPase genes are down-regulated during adipose stem cell differentiation

Acosta, Elisa Slater

doi:10.2741/326

Cited by 4 publications

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“…Taken together, these studies and our results indicate that the level of expression and the location of the β subunit in epithelial cells are important for maintaining their well-differentiated phenotype, which disappears during cancer progression. Research published by our group on sodium pump isoform expression levels in stem cells has confirmed that adipose-derived mesenchymal stem cells express all known Na,K-ATPase isoforms, but some of these genes are turned off along differentiation (Acosta et al, 2011 ). In CRC cells or its metastases in liver we have never seen expression of all isoforms, rather, we have detected the expression signatures specified in Table 2 .…”

Section: Discussionmentioning

confidence: 95%

Na,K-ATPase Isozymes in Colorectal Cancer and Liver Metastases

et al. 2016

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The goal of this study was to define Na,K-ATPase α and β subunit isoform expression and isozyme composition in colorectal cancer cells and liver metastases. The α1, α3, and β1 isoforms were the most highly expressed in tumor cells and metastases; in the plasma membrane of non-neoplastic cells and mainly in a cytoplasmic location in tumor cells. α1β1 and α3β1 isozymes found in tumor and metastatic cells exhibit the highest and lowest Na+ affinity respectively and the highest K+ affinity. Mesenchymal cell isozymes possess an intermediate Na+ affinity and a low K+ affinity. In cancer, these ions are likely to favor optimal conditions for the function of nuclear enzymes involved in mitosis, especially a high intra-nuclear K+ concentration. A major and striking finding of this study was that in liver, metastasized CRC cells express the α3β1 isozyme. Thus, the α3β1 isozyme could potentially serve as a novel exploratory biomarker of CRC metastatic cells in liver.

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Section: Discussionmentioning

confidence: 95%

Na,K-ATPase Isozymes in Colorectal Cancer and Liver Metastases

et al. 2016

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“…Cardiomyocytes derived from mouse embryonic stem cells have been demonstrated to exhibit a time-dependent expression of ion channels 46 . Expression and distribution of Na + , K + -ATPase subunits are changed during differentiation of pluripotentent embryonic stem cells 47,48 . It has been revealed that stem cells could increase the activities of the sarcolemma Na + , K + -ATPase and the sarcoplasmic reticulum membrane Ca 2+ -ATPase in heart failure, a possible mechanism to improve heart function 49,50 .…”

Section: Discussionmentioning

confidence: 99%

Proliferation-related changes in K+ content in human mesenchymal stem cells

Marakhova

Домнина

Shatrova

et al. 2019

Sci Rep

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Intracellular monovalent ions have been shown to be important for cell proliferation, however, mechanisms through which ions regulate cell proliferation is not well understood. Ion transporters may be implicated in the intracellular signaling: Na+ and Cl− participate in regulation of intracellular pH, transmembrane potential, Ca2+ homeostasis. Recently, it is has been suggested that K+ may be involved in “the pluripotency signaling network”. Our study has been focused on the relations between K+ transport and stem cell proliferation. We compared monovalent cation transport in human mesenchymal stem cells (hMSCs) at different passages and at low and high densities of culture as well as during stress-induced cell cycle arrest and revealed a decline in K+ content per cell protein which was associated with accumulation of G1 cells in population and accompanied cell proliferation slowing. It is suggested that cell K+ may be important for successful cell proliferation as the main intracellular ion that participates in regulation of cell volume during cell cycle progression. It is proposed that cell K+ content as related to cell protein is a physiological marker of stem cell proliferation and may be used as an informative test for assessing the functional status of stem cells in vitro.

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“…Taken together, these studies and our results indicate that the level of expression and the location of the β subunit in epithelial cells are important for maintaining their well-differentiated phenotype, which disappears during cancer progression. Research published by our group on sodium pump isoform expression levels in stem cells has confirmed that adipose-derived mesenchymal stem cells express all known Na,K-ATPase isoforms, but some of these genes are turned off along differentiation (Acosta et al, 2011). In CRC cells or its metastases in liver we have never seen expression of all isoforms, rather, we have detected the expression signatures specified in Table 2.…”

Section: Discussionmentioning

confidence: 57%

Alterations in gene expression induced by oxaliplatin based chemotherapy in colorectal cancer

Rotoli¹

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Na+, K+-ATPase genes are down-regulated during adipose stem cell differentiation

Cited by 4 publications

References 0 publications

Na,K-ATPase Isozymes in Colorectal Cancer and Liver Metastases

Na,K-ATPase Isozymes in Colorectal Cancer and Liver Metastases

Proliferation-related changes in K+ content in human mesenchymal stem cells

Alterations in gene expression induced by oxaliplatin based chemotherapy in colorectal cancer

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