Na+-independent, electrogenic Cl- uptake across the posterior gills of the Chinese crab (Eriocheir sinensis): Voltage-clamp and microelectrode studies

Onken, Horst; Graszynski, Kai; Zeiske, Wolfgang

doi:10.1007/bf00262311

Cited by 54 publications

(47 citation statements)

References 21 publications

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“…Equally effective were internal diphenylamine-2-carboxylate (DPC, inhibitor of Cl--channels) or acetazolamide (inhibitor of the carbonic anhydrase). However, internal ouabain, the well-known inhibitor of the Na+/K+-ATPase did not reduce C1-current (Onken et al, 1991).…”

Section: Eriocheir Sinensis: a Model For Crabs Which Conquered Freshwmentioning

confidence: 80%

“…Short-circuiting of Gt amounted to about 3 mS/ crn 2 under these conditions (Onken et al, 1991). The negative current, which even became more negative after external substitution of Na + (choline), depended on the presence of C1-in the external bath and obviously represented a Na+-independent electrogenic C1 uptake.…”

Section: Eriocheir Sinensis: a Model For Crabs Which Conquered Freshwmentioning

confidence: 90%

“…Only by using this method has it become possible to define ion-specific and arearelated currents and conductances in great detail: (a) unlike the PD which is a qualitative measure, quantitative parameters were obtained (Schwarz & Graszynski, 1989;Onken et al, 1991;Zeiske et al, 1992;Onken & Siebers, 1992); (b) cell impalements with microelectrodes via the basolateral membrane allowed the investigation of membrane potentials and conductances (Onken et al, 1991), and (c) application of current-noise analysis resulted in the unambiguous demonstration of Na+-channels in the apical gill membrane of the Chinese crab (Zeiske et al, 1992).…”

Section: Electrophysiological Methods In Crab Gill Investigationsmentioning

confidence: 99%

“…In the following, we focus on basic data and those results which are important to understand the proposed transport model shown in Figure 4. More detailed information, like kinetics or regulation of NaC1 uptake, can be found elsewhere (Onken, 1989;Schwarz, 1990;Onken et al, 1991;Riestenpatt et al, 1992;Zeiske et al, 1992).…”

Section: Eriocheir Sinensis: a Model For Crabs Which Conquered Freshwmentioning

confidence: 99%

“…This has become possible in the last few years by the application of modern electrophysiological techniques to isolated epithehal layers which were mounted in an Ussingchamber (Schwarz & Graszynski, 1989;Onken, 1989;Schwarz, 1990;Onken et al, 1991;Zeiske et al, 1992;Onken & Siebers, 1992).…”

Section: Introductionmentioning

confidence: 99%

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How to overcome osmotic stress? Marine crabs conquer freshwater. New insights from modern electrophysiology

Onken

Graszynski

Johannsen

et al. 1995

Helgolander Meeresunters

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In the present article we review our findings on split lamella preparations of crab gills mounted in modified Ussing-chambers with respect to mechanistic and ecophysiological aspects. The leaky gill epithelium of shore crabs adapted to brackish water absorbs Na + and C1-in a coupled mode, and shows similarities to other salt-absorbing epithelia exposed to moderately diluted media. The results so far obtained for NaCI uptake across the gills of the shore crab are compatible with a transport model where two cell types operate in parallel, one displaying cotransport-like NaC1 absorption, similar to that in the thick ascending limb of Henle's loop of the mammalian mephron, and the other one with characteristics of amiloride-sensitive, channel-mediated Na + uptake by frog skin. Although there is no clear evidence for the apical mechanisms in this model, it may serve as a good basis for more detailed studies in the future. The moderately tight gill epithelium of freshwater adapted Chinese crabs absorbs Na + and C1-independently from each other, and shows similarities to other salt-absorbing epithelia exposed to freshwater. The characteristics of a positive, Na +-dependent short-circuit current with externally C1--free saline indicate that active Na + uptake proceeds in a frog-skin-like mode via apical Na+-channels and the basolateral Na+/K+opump. The nature of a negative short-circuit current with external C1--saline indicates that active and Na +-independent CI-uptake is driven by an apical V-type H~--pump and proceeds via apical C1-/ HCO3--exchange and basolateral C1--channels.

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Section: Eriocheir Sinensis: a Model For Crabs Which Conquered Freshwmentioning

confidence: 80%

Section: Eriocheir Sinensis: a Model For Crabs Which Conquered Freshwmentioning

confidence: 90%

Section: Electrophysiological Methods In Crab Gill Investigationsmentioning

confidence: 99%

Section: Eriocheir Sinensis: a Model For Crabs Which Conquered Freshwmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

How to overcome osmotic stress? Marine crabs conquer freshwater. New insights from modern electrophysiology

Onken

Graszynski

Johannsen

et al. 1995

Helgolander Meeresunters

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Osmoregulation and Excretion

Larsen

Deaton

Onken

et al. 2014

Comprehensive Physiology

179

141

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The article discusses advances in osmoregulation and excretion with emphasis on how multicellular animals in different osmotic environments regulate their milieu intérieur. Mechanisms of energy transformations in animal osmoregulation are dealt with in biophysical terms with respect to water and ion exchange across biological membranes and coupling of ion and water fluxes across epithelia. The discussion of functions is based on a comparative approach analyzing mechanisms that have evolved in different taxonomic groups at biochemical, cellular and tissue levels and their integration in maintaining whole body water and ion homeostasis. The focus is on recent studies of adaptations and newly discovered mechanisms of acclimatization during transitions of animals between different osmotic environments. Special attention is paid to hypotheses about the diversity of cellular organization of osmoregulatory and excretory organs such as glomerular kidneys, antennal glands, Malpighian tubules and insect gut, gills, integument and intestine, with accounts on experimental approaches and methods applied in the studies. It is demonstrated how knowledge in these areas of comparative physiology has expanded considerably during the last two decades, bridging seminal classical works with studies based on new approaches at all levels of anatomical and functional organization. A number of as yet partially unanswered questions are emphasized, some of which are about how water and solute exchange mechanisms at lower levels are integrated for regulating whole body extracellular water volume and ion homeostasis of animals in their natural habitats. © 2014 American Physiological Society.

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Mechanisms of Excretion and Ion Transport in Invertebrates

O’Donnell

1997

Comprehensive Physiology

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The sections in this article are: Ion Transport and Osmoregulation in Invertebrates: Functional Morphology and Cellular Mechanisms Water Expulsion Vesicles and Contractile Vacuoles in Protozoans, Sponges, and Cnidarians Nephridia and Coelomoducts: Embryology and Terminology Nephridia Coelomoduct‐Derived Renal Organs Gut‐Derived Renal Organs Other Ion‐Transporting Structures Excretion in Invertebrates Nitrogenous Wastes Alkaloids Organic Anions Organic Cations Magnesium and Sulfate Storage and Deposit Excretion Catabolism of Insect Neurohormones by Malpighian Tubules Roles of Arthropod Excretory Systems and Molluscan Mantle in Acid‐Base Regulation Active Transport of Sugars Transport of Cardiac Glycosides Passive Permeability to Metabolites and Toxins Future Research

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Na+-independent, electrogenic Cl- uptake across the posterior gills of the Chinese crab (Eriocheir sinensis): Voltage-clamp and microelectrode studies

Cited by 54 publications

References 21 publications

How to overcome osmotic stress? Marine crabs conquer freshwater. New insights from modern electrophysiology

How to overcome osmotic stress? Marine crabs conquer freshwater. New insights from modern electrophysiology

Osmoregulation and Excretion

Mechanisms of Excretion and Ion Transport in Invertebrates

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