Na+-dependent inactivation of vascular Na+/Ca2+ exchanger responsible for reduced peripheral blood flow in neuropathic pain model

Enhanced Contraction of Arterial Smooth Muscle Cell in Skin Artery Is Sensitive to Hyperpolarization Mediated by BK_Ca Channel in Chronic Constriction Injury Model Rat

“…Previous our study showed that improvement of reduced skin blood flow by vasodilators is correlated with alleviation of neuropathic pain in CCI mice and enhanced cutaneous vasocontraction is caused by NCX inactivation following reduction of skin blood flow in CCI rats. 5,11) The present study found that the effect of vasodilators, which increase mediated by G s protein coupling receptors or cGMP to ipsilateral plantar arterial contraction is potent than the that to contralateral plantar arterial contraction in CCI rats. In addition to it, the enhanced vasorelaxation caused by increased cAMP or cGMP is followed by activation of BK Ca channel.…”

“…Previous our study found that enhanced Ca 2+ elevation caused by decreased NCX activity is a key player of enhanced vasocontraction in CCI model: NCX inhibitor scarcely affected enhanced contraction in ipsilateral plantar artery while contralateral plantar arterial condition was mimicked to the ipsilateral condition by the NCX inhibitor. 11) In other words, even weak Ca 2+ influx can cause sufficient Ca 2+ elevation. In this study result, we found that contribution of voltage-dependent Ca 2+ channel to NAd-induced contraction in ipsilateral side is larger than the that in contralateral side.…”

“…We have found that the inactivation of sodium calcium exchanger (NCX) caused by decrease of Na + gradient due to enhanced Na + influx mediated by amiloride-sensitive pathway contributes to the Ca 2+ elevation, which leads to enhanced plantar arterial contraction in CCI model rats. 11) Thus, normalizing the regulatory mechanisms of calcium concentration would be a good target. Based on our previous results, it is plausible to say that enhanced vasocontraction is caused by intracellular molecular signaling and cAMP would suppress the enhanced vasocontraction by inhibiting some proteins involving in the enhanced intracellular signaling.…”

Longitudinal analysis of microcirculatory parameters in gingival tissues after tooth extraction in patients with different risk profiles for wound healing disorders – a pilot study

“… In order to produce valid values under standardized conditions, as described in the literature, care was taken with each patient to include a 15-minute resting phase before the start of the measurements so that the blood pressure could settle at a constant level [ 26 ]. Furthermore, since both cold and pain can lead to peripheral vasoconstriction, a comfortable temperature as well as freedom from pain during the measurements were ensured [ 27 , 28 ]. The measurements were performed with a type LF-2 probe of the micro-lightguide spectrophotometer O2C (Oxygen to See, LEA Medizintechnik, Gießen, Germany; see Fig.…”