N6-methyladenosine RNA methylation regulators contribute to the progression of prostate cancer

Wu, Qunying; Xie, Xing; Huang, Yiming; Meng, Shanshan; Li, You-Cheng; Wang, Huifeng; Hu, Yanling

doi:10.7150/jca.46379

Cited by 35 publications

(38 citation statements)

References 37 publications

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“…Another research has shown that YTHDF1/2, YTHDC2, and METTL3 were upregulated in PCa, but METTL14, FTO, and ALKBH5 were down-regulated. In addition, the results found that YTHDF1, YTHDF2, and YTHDC2 exhibited a positive correlation, but METTL14, FTO, and ALKBH5 were negatively correlated to Gleason grades (Wu et al, 2021). Some researchers analyzed the important m6A RNA regulators, which act as prognosis factors in prostate cancer, and identified RBMX, NXF1, YTHDF1, HNRNPA2B1, and TRMT112 as critical genes that have great effects on the prognosis of PCa patients (Xu et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

RNA m6A Methylation Regulators Multi-Omics Analysis in Prostate Cancer

Wang

2021

Front. Genet.

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RNA N6-methyladenosine (m6A) methylation is known to be the most popular RNA modification in animals. Many research reports have elaborated on the effects of m6A regulators in medical practice, such as diagnosis, prognosis, and treatment. M6A modification has evident impacts on many aspects of RNA metabolism, just like RNA splicing, processing, translation, and stability. M6A also has a magnificent role in numerous types of cancers. We analyzed the prostate cancer datasets, from The Cancer Genome Atlas (TCGA) database, for every recognized m6A regulator in their gene expression, DNA methylation status and copy number variations (CNVs). We also systematically analyzed the relationship between different m6A regulators and the prognosis of prostate cancer. The results illustrated considerable differences in the expression of various m6A regulators between the prostate and normal cancer samples. At the same time, there were evident differences in the expression of various m6A regulators in prostate cancers with different Gleason scores. Subsequently, we determined CBLL1, FTO, YTHDC1, HNRNPA2B1 as crucial m6A regulators of prostate cancer. Premised on the expression of CBLL1, we also identified potential therapeutic agents for prostate cancer, and knockdown of HNRNPA2B1 prominently inhibited prostate cells migration and invasion in vitro experiment.

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Section: Discussionmentioning

confidence: 99%

RNA m6A Methylation Regulators Multi-Omics Analysis in Prostate Cancer

Wang

2021

Front. Genet.

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“…YTHDF1 is a "reader" of m6A-modified mRNA (Wang et al 2015). The change in m6A levels may be involved in PCa occurrence and development (Wu et al 2021). Previous studies have reported that YTHDF1 promotes the occurrence and metastasis of gastric cancer in an m6Adependent manner by promoting the translation of USP14 and it may represent a potential target for gastric cancer treatment (Chen et al 2021c).…”

Section: Discussionmentioning

confidence: 99%

YTHDF1 promotes the proliferation, migration, and invasion of prostate cancer cells by regulating TRIM44

Chen

Feng

et al. 2021

Genes Genom

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Background Prostate cancer (PCa) is one of the most common malignancies in men. YTHDF1 may play an important role in promoting PCa progression, but there is no reports to date on YTHDF1 function in PCa. Objective This study explored whether YTHDF1 could regulate TRIM44 in PCa cells. Methods By querying the TCGA database, we evaluated YTHDF1 expression in PCa, the OS and DFS of YTHDF1, and the correlation between YTHDF1 and TRIM44 in PCa. We constructed vectors to interfere with YTHDF1 expression and overexpress TRIM44 to examine the role of YTHDF1 and TRIM44 in PCa cells. Differentially expressed mRNAs were identified by mRNA sequencing. The levels of YTHDF1, TRIM44, LGR4, SGTA, DDX20, and FZD8 were measured by qRT-PCR and WB was used to determine YTHDF1 and TRIM44 expression. A CCK-8 assay was used to assess cell proliferation. A Transwell chamber assay was used measure cell migration and invasion ability. Results YTHDF1 was highly expressed in both Pca tissues and cells. PCa patient prognosis with high YTHDF1 expression was relatively poor. Cell function experiments showed that inhibiting YTHDF1 expression decreased cell proliferation, migration, and invasion. RNA sequencing analysis revealed that YTHDF1 may promote PCa cell proliferation, migration, and invasion by modulating TRIM44 expression. Cell function experiments further verified that YTHDF1 promoted PCa cell proliferation, migration, and invasion by regulating TRIM44. Conclusions YTHDF1 enhances PCa cell proliferation, migration, and invasion by regulating TRIM44.

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“…Interestingly, YTHDF1 is remarkably higher in lymph node metastasis than in castration-resistant prostate cancer with bone metastasis. 70 …”

Section: The Roles Of Ythdf1 In Tumorsmentioning

confidence: 99%

The roles and mechanisms of the m6A reader protein YTHDF1 in tumor biology and human diseases

Chen

Zhong

Xia

et al. 2021

Molecular Therapy - Nucleic Acids

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N6-methyladenosine RNA methylation regulators contribute to the progression of prostate cancer

Cited by 35 publications

References 37 publications

RNA m6A Methylation Regulators Multi-Omics Analysis in Prostate Cancer

RNA m6A Methylation Regulators Multi-Omics Analysis in Prostate Cancer

YTHDF1 promotes the proliferation, migration, and invasion of prostate cancer cells by regulating TRIM44

The roles and mechanisms of the m6A reader protein YTHDF1 in tumor biology and human diseases

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