N6-methyladenosine in poly(A) tails stabilize VSG transcripts

Viegas, Idálio J; Macêdo, Juan Pereira de; Serra, Lúcia; Niz, Mariana De; Temporão, Adriana; Pereira, Sara Silva; Mirza, Aashiq H.; Bergström, Edmund T.; Rodrigues, João; Aresta‐Branco, Francisco; Jaffrey, Samie R.; Figueiredo, Luísa M.

doi:10.1038/s41586-022-04544-0

Cited by 39 publications

(43 citation statements)

References 41 publications

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“…This group also found longer half-lives associated with m 6 A containing transcripts [ 36 ]. Further validation later came independently from another group who revealed m 6 A being located in the poly(A) tail with a 16-mer motif located upstream in the 3′ UTR [ 74 ]. The link between m 6 A in the 3′ end and degradation was addressed in the context of VSGs transcripts [ 74 ].…”

Section: Epitranscriptomics Of Mrna and Noncoding Rna Processing In P...mentioning

confidence: 99%

Epitranscriptomics in parasitic protists: Role of RNA chemical modifications in posttranscriptional gene regulation

et al. 2022

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“Epitranscriptomics” is the new RNA code that represents an ensemble of posttranscriptional RNA chemical modifications, which can precisely coordinate gene expression and biological processes. There are several RNA base modifications, such as N6-methyladenosine (m6A), 5-methylcytosine (m5C), and pseudouridine (Ψ), etc. that play pivotal roles in fine-tuning gene expression in almost all eukaryotes and emerging evidences suggest that parasitic protists are no exception. In this review, we primarily focus on m6A, which is the most abundant epitranscriptomic mark and regulates numerous cellular processes, ranging from nuclear export, mRNA splicing, polyadenylation, stability, and translation. We highlight the universal features of spatiotemporal m6A RNA modifications in eukaryotic phylogeny, their homologs, and unique processes in 3 unicellular parasites—Plasmodium sp., Toxoplasma sp., and Trypanosoma sp. and some technological advances in this rapidly developing research area that can significantly improve our understandings of gene expression regulation in parasites.

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Section: Epitranscriptomics Of Mrna and Noncoding Rna Processing In P...mentioning

confidence: 99%

Epitranscriptomics in parasitic protists: Role of RNA chemical modifications in posttranscriptional gene regulation

et al. 2022

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“…Taken together, these experiments suggest that formation of the multi-condensate is functionally linked to the processes of efficient expression site transcription and antigen mRNA processing. In addition, m 6 A RNA modifications were recently detected in the Poly-A tail of the active antigen transcript ( Viegas et al., 2022 ), which are responsible for increased antigen mRNA stability. m 6 A modifications have been implicated in condensate formation ( Ries et al., 2019 ) and it remains to be shown if such RNA modifications play a role in the multi-condensate formation process.…”

Section: Potential Condensate Seed Factors Involved In Parasite Immun...mentioning

confidence: 99%

“…To ensure stable levels of antigen mRNA, the active ES is expressed by highly processive RNA polymerase I (Pol I) within a nuclear body referred to as expression site body (ESB) ( Navarro and Gull, 2001 ). Trans -splicing ensures maturation of the polycistronic pre-mRNA and mature antigen mRNA is additionally stabilized by m 6 A RNA modifications in the poly-A tail ( Viegas et al., 2022 ). Taken together, trypanosomes have evolved an entire ensemble of molecular mechanisms that ensure extremely high levels of antigen mRNA, organized in a multi-step expression process.…”

Section: Introductionmentioning

confidence: 99%

Nuclear Condensates: New Targets to Combat Parasite Immune Evasion?

Luzak

2022

Front. Cell. Infect. Microbiol.

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“…The infectious disease dynamics model is commonly used to estimate and forecast the number of infections of contagious diseases such as SARS and the COVID-19 outbreak. (Cori et al, 2009;Fukutome et al, 2007;Fang et al, 2006;Read et al, 2020 andViegas et al, 2020). This study provides a technique for calculating epidemic reproduction numbers.…”

Section: Introductionmentioning

confidence: 95%

Mathematical modeling on the transmission of COVID-19 and its reproduction numbers in SAARC countries

Uddin

Parvez

Ali

et al. 2022

JANS

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In the middle of December 2019, a virus known as coronavirus (COVID-19) generated by severe acute respiratory syndrome corona virus 2 (SARC-CoV-2) was first detected in Wuhan, Hubei Province, China. As of the 9th of March, 2022, spread to over 212 countries, causing 429 million confirmed cases and 6 million people to lose their lives worldwide. In developing countries like the South Asian area, alarming dynamic variations in the pattern of confirmed cases and death tolls were displayed. During epidemics, accurate assessment of the characteristics that characterize infectious disease transmission is critical for optimizing control actions, planning, and adapting public health interventions. The reproductive number, or the typical number of secondary cases caused by an infected individual, can be employed to determine transmissibility. Several statistical and mathematical techniques have been presented to calculate across the duration of an epidemic. A technique is provided for calculating epidemic reproduction numbers. It is a MATLAB version of the EpiEstim package's R function estimate R, version 2.2-3. in the South Asian Association for Regional Cooperation (SAARC) countries. The three methodologies supported are 'parametric SI,' 'non-parametric SI,' and 'uncertain SI.' The present study indicated that the highest reproduction number was 12.123 and 11.861 on 5th and 14th March 2020 in India and Sri_Lanka, whereas the lowest reproduction number was the lowest was 0.300 and 0.315 in Sri_Lanka and India. The Maximum and minimum reproductive number of Bangladesh was 3.752 and 0.725. In this study, we have tried to point out the worst, best and current situation of SAARC countries.

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N6-methyladenosine in poly(A) tails stabilize VSG transcripts

Cited by 39 publications

References 41 publications

Epitranscriptomics in parasitic protists: Role of RNA chemical modifications in posttranscriptional gene regulation

Epitranscriptomics in parasitic protists: Role of RNA chemical modifications in posttranscriptional gene regulation

Nuclear Condensates: New Targets to Combat Parasite Immune Evasion?

Mathematical modeling on the transmission of COVID-19 and its reproduction numbers in SAARC countries

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