Untitled

Abstract

The seasonal changes in the ovary of T. schirnerae were described based on histological and histochemical analyses.The development of oocytes was divided into ten stages: oogonia, chromatin nucleolus (1 to 3), perinuclear, primary vitellogenic, secondary vitellogenic (1 to 3) and tertiary vitellogenic. The seasonal ovarian development was classified into six phases: proliferation, previtellogenic, primary vitellogenic, secondary vitellogenic, tertiary vitellogenic and oosorption.Yolk accumulation started during primary vitellogenesis, when basophilic small yolk globules appeared at the cortical cytoplasmic region. The highly basophilic large yolk globules of secondary vitellogenic stage 1 underwent a series of morphological changes to become mildly basophilic yolk platelets in secondary vitellogenic stage 2. The ooplasm noted with strongly acidophilic large yolk platelets in secondary vitellogenic stage 3. The entire ooplasm formed an acidophilic, homogeneous matrix during tertiary vitellogenic stage. The primary vitellogenic stage oocytes showed a strong positive reaction to PAS and MBB, indicating the glycoproteinaceous nature of the yolk. In the secondary and tertiary vitellogenic oocytes, yolk platelets showed positive reaction for MBB, PAS and Sudan black B, demonstrating the lipoglycoproteinaceous nature of the yolk. In T. schirnerae, vitellogenesis extended from October to March and spawning occurred in April. It is concluded that T. schirnerae is an annual breeder accommodating a single ovarian cycle during the intermoult period.

Prevalence, site of infection, and differentiating oocytes of the cymothoid isopod, Norileca indica (Milne Edwards, 1840) from its infected short mackerel, Rastrelliger brachysoma, in the Upper Gulf of Thailand

“…Based on considerable differences in cell size, histological staining properties and histological features, differentiation of oocyte was classified into three major stages: oogonium stage, primary oocyte stage and secondary oocyte stage ( Figures 1D-1F). This pattern of oocyte differentiation was similar to previous observations in Bopyrina abbreviata (Romero-Rodriguez et al, 2017) and other crustaceans (Krol et al, 1992;Van Herp & Soyes, 1997;Smija & Devi, 2015). However, some studies showed that the five distinguished stages of ovarian maturation were identified in penaeid shrimps (Tan-Fermin, 1991;Medina et al, 1996).…”

Reproduction in the spider crab Maiopsis panamensis (Decapoda: Majoidea) in the Gulf of California

“…The early vitellogenic oocytes, which were present in Stage II and observed with light microscopy, have been described in TEM by Mollemberg et al (2017), who reported that in other Majoidea, no evidence of the chorion was observed, and oocytes were surrounded by follicular cells. Our observation that triglycerides increase and phospholipids decrease during vitellogenesis and post-vitellogenesis is consistent with patterns observed by Arcos-Ortega et al (2019) in The description of ovarian development in this study used quantitative and qualitative characteristics of histological and histochemical analyses, which were similar to methods used in other species of decapod crustaceans, such as the shrimp Penaeus japonicus (Yano, 1988), the lobster Panulirus japonicus (Minagawa & Sano, 1997), the anomuran crab Clibanarius clibanarius (Varadarajan & Subramoniam, 1980), and the brachyuran crab Travancoriana schirnerae (Smija & Sudha, 2015) to describe gonadal development.…”

“…These types of descriptions are useful to define the most accurate ovarian maturation scale, as Yano ( 1988) did for P. japonicus and Varadarajan and Subramoniam (1980) remarkable; the very pronounced increase in oocyte diameter from pre-vitellogenesis to ovulation was consistent with studies in other decapods (Minagawa & Sano, 1997;Smija & Sudha, 2015;Tan-Fermin & Pudadera, 1989;Varadarajan & Subramoniam, 1980;Yano, 1988). Oocyte diameter reflects yolk accumulation in wild or captive populations and is considered to be the best reflection of ovary condition in decapods (Arcos et al, 2005;Minagawa & Sano, 1997;Smija & Sudha, 2015;Tan-Fermin & Pudadera, 1989;Yano, 1988 (Varadarajan & Subramoniam, 1980).…”

“…To classify the ovarian stages and different oocyte substages, previous descriptions for crabs (Santos et al, 2009;Smija & Sudha, 2015;Zara et al, 2011), prawns (Kodama et al, 2009; Tan-Fermin & F I G U R E 1 Total sampling area of the Pacific spider crab Maiopsis panamensis in the Gulf of California, Mexico Pudadera, 1989;Yano, 1988), lobsters (Minagawa & Sano, 1997), spider crabs (Rotllant et al, 2007), and other majoids (Mollemberg et al, 2017) Only oocytes cut approximately along the medial transverse plane and exhibiting nucleoplasm were considered for assessing gonadal development stage and for the morphometric and statistical analysis (Palacios et al, 1999).…”