N1-methylspermidine, a stable spermidine analog, prolongs anagen and regulates epithelial stem cell functions in human hair follicles

Ramot, Yuval; Mora, Barbara; Pinto, Daniela; Kloepper, Jennifer E.; Paus, Ralf

doi:10.1007/s00403-015-1592-9

Cited by 14 publications

(15 citation statements)

References 20 publications

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“…Some studies have reported that polyamines play an important role in animal hair growth, that inhibition of polyamine synthesis regulates murine hair growth and that polyamines also help determine hair growth in sheep hair follicles . Recently, the correlation between hair loss and polyamines has received increasing attention . Polyamines are also associated with inflammation.…”

Section: Introductionmentioning

confidence: 99%

“…14 Recently, the correlation between hair loss and polyamines has received increasing attention. [15][16][17] Polyamines are also associated with inflammation. A study comparing polyamine metabolism in synovial fibroblasts between patients with rheumatoid arthritis and those with general osteoarthritis reported that SPD/spermine N1-acetyltransferase, polyamine-modulated factor 1-binding protein 1 and S-adenosyl methionine decarboxylase were upregulated.…”

Section: Introductionmentioning

confidence: 99%

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Altered polyamine profiling in the hair of patients with androgenic alopecia and alopecia areata

Lee

Lew²,

Sim³

et al. 2019

The Journal of Dermatology

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Hair follicles are among the most highly proliferative tissues. Polyamines are associated with proliferation, and several polyamines including spermidine and spermine play anti‐inflammatory roles. Androgenic alopecia results from increased dihydrotestosterone metabolism, and alopecia areata is an autoimmune disease. This study aimed to investigate differences in polyamine profiles in hair samples between patients with androgenic alopecia and alopecia areata. Polyamine concentrations were determined through high‐performance liquid chromatography‐mass spectrometry. Hair samples were derivatized with isobutyl chloroformate. Differences in polyamine levels were observed between androgenic alopecia and alopecia areata compared with normal controls. In particular, polyamine levels were higher in alopecia areata patients than in normal controls. Certain polyamines displayed different concentrations between the androgenic alopecia and alopecia areata groups, suggesting that some polyamines, particularly N‐acetyl putrescine (P = 0.007) and N‐acetyl cadaverine (P = 0.0021), are significantly different in androgenic alopecia. Furthermore, spermidine (P = 0.021) was significantly different in alopecia areata. Our findings suggest that non‐invasive quantification of hair polyamines may help distinguish between androgenic alopecia and alopecia areata. Our study provides novel insights into physiological alterations in patients with androgenic alopecia and those with alopecia areata and reveals some differences in polyamine levels in hair loss diseases with two different modes of action.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Altered polyamine profiling in the hair of patients with androgenic alopecia and alopecia areata

Lee

Lew²,

Sim³

et al. 2019

The Journal of Dermatology

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“…To assess, finally, whether theophylline exerts any effects on epithelial stem cells in aged human epidermis, we evaluated the IR of the epithelial stem cell marker, K15, . In both tested concentration, theophylline significantly increased K15 IR in the basal layer of the epidermis (Fig.…”

Section: Resultsmentioning

confidence: 99%

Theophylline exerts complex anti‐ageing and anti‐cytotoxicity effects in human skin ex vivo

Bertolini

Ramot

Gherardini

et al. 2019

Intern J of Cosmetic Sci

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OBJECTIVE: Theophylline is a phosphodiesterase inhibitor that is being used clinically for asthma therapy. In addition, it is recognized as a cosmetic agent with possible anti-ageing and anti-oxidative properties. Nevertheless, how it affects human skin is still poorly examined. METHODS: Theophylline (10 or 100 µM) was administered to the culture medium of full-thickness human skin ex vivo for 24 or 72 h. RESULTS: Theophylline stimulated protein expression of the antioxidant metallothionein-1 and mRNA levels of collagen I and III. Assessment of fibrillin-1 immunohistology revealed enhanced structural stability of dermal microfibrils. Theophylline also exerted extracellular matrix-protective effects by decreasing MMP-2 and MMP-9 mRNA levels, partially antagonizing the effects of menadione, the potent, toxic ROS donor. In addition, it decreased menadione-stimulated epidermal keratinocytes apoptosis. Interestingly, theophylline also increased the level of intracutaneously produced melatonin, that is the most potent ROS-protective and DNA damage repair neuromediator, and tendentially increased protein expression of MT1, the melatonin receptor. Theophylline also increased the expression of keratin 15, the stem cell marker, in the epidermal basal layer but did not change mitochondrial activity or epidermal pigmentation. CONCLUSION: This ex vivo pilot study in human skin shows that theophylline possesses several interesting complex skin-protective properties. It encourages further examination of theophylline as a topical candidate for anti-ageing treatment. R esum e OBJECTIF: la th eophylline est un inhibiteur de la phosphodiest erase actuellement utilis ee en clinique pour le traitement de l'asthme. En outre, elle est reconnue comme etant un agent cosm etique ayant des propri et es potentiellement anti-âge et antioxydantes. Cependant, la mani ere dont elle affecte la peau chez l'homme est encore tr es peu etudi ee. M ETHODES: de la th eophylline (10 ou 100 lM) a et e ajout ee dans le milieu de culture d'un echantillon de peau humaine d' epaisseur totale ex vivo pendant 24 ou 72 h. Theophylline effects in human skin ex vivo M. Bertolini et al. 84 Theophylline effects in human skin ex vivo M. Bertolini et al. Theophylline effects in human skin ex vivo M. Bertolini et al. Theophylline effects in human skin ex vivo M. Bertolini et al.

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“…For example, inhibiting polyamine synthesis significantly regulates murine hair growth, and polyamines play an essential role in widening sheep hair follicles . Recently, polyamines have gained attention for their role in hair growth; spermidine has been reported to promote hair growth from human epithelial stem cells …”

Section: Premisesmentioning

confidence: 99%

Distribution of polyamines may be altered in different scalp regions of patients with hair loss

Lee

Lew

et al. 2019

Experimental Dermatology

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Hair loss, from the vertex or front of the head, generally occurs due to increased androgenic steroid levels. Androgenic steroids, particularly testosterone and dihydrotestosterone, are distributed differently across the vertex and occipital regions and are involved in inducing ornithine decarboxylase expression. Therefore, we hypothesized that the distribution of polyamines may be altered in different scalp regions. For the overall metabolic profiling of polyamines in patients with hair loss, a liquid chromatography‐mass spectrometry was used. We investigated the differential polyamine levels in different regions of the hair of patients with male pattern baldness and those with female pattern hair loss. The levels of most polyamines were higher in the vertex region than in the occipital region, and N‐acetyl polyamine levels differed significantly. We proposed to test our hypothesis by profiling polyamines in human hair fibre to evaluate the distribution of metabolites in various regions of the scalp.

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N1-methylspermidine, a stable spermidine analog, prolongs anagen and regulates epithelial stem cell functions in human hair follicles

Cited by 14 publications

References 20 publications

Altered polyamine profiling in the hair of patients with androgenic alopecia and alopecia areata

Altered polyamine profiling in the hair of patients with androgenic alopecia and alopecia areata

Theophylline exerts complex anti‐ageing and anti‐cytotoxicity effects in human skin ex vivo

Distribution of polyamines may be altered in different scalp regions of patients with hair loss

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