N-terminal amino acid sequences of ω-gliadins and ω-secalins

Kasarda, Donald D.; Autran, J.C.; Lew, Ellen J-L.; Nimmo, C. C.; Shewry, Peter R.

doi:10.1016/0167-4838(83)90132-2

Cited by 200 publications

(58 citation statements)

References 35 publications

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“…Comparison of the N-terminal amino acid sequences also showed some variation (especially in the chromosome lB-encoded w-gliadins), but all were clearly homologous [69].…”

Section: Hmw Prolamins Of Barley and Ryementioning

confidence: 96%

The prolamin storage proteins of cereal seeds: structure and evolution

Shewry

Tatham

1990

Biochemical Journal

637

421

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“…Comparison of the N-terminal amino acid sequences also showed some variation (especially in the chromosome lB-encoded w-gliadins), but all were clearly homologous [69].…”

Section: Hmw Prolamins Of Barley and Ryementioning

confidence: 96%

The prolamin storage proteins of cereal seeds: structure and evolution

Shewry

Tatham

1990

Biochemical Journal

637

421

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“…No sequence data of ω-gliadin genes was available. We conjectured that the nucleotide sequences were more or less similar among the structural genes for ω-gliadins, ω-secalins, and C-hordeins because all of them encode ω-type prolamins and are suggested to have arisen from a common ancestral gene (Kasarda et al, 1983). The designed primers (o-sec-upp and o-sec-low, Table 2) were expected to amplify about 1.7 kbp of the ω-secalin sequence.…”

Section: Resultsmentioning

confidence: 99%

“…These include the high molecular weight (HMW) secalins, 40K γ-secalins, 75K γ-secalins, and ω-secalins Kasarda et al, 1983;Shewry et al, 1982Shewry et al, , 1984. The structural genes for the ω-secalins and 40K γ-secalines are located on the short arm of rye chromosome 1R (1RS) (Lawrence and Shepherd, 1981), and are tightly linked to each other to be given a locus name, Sec-1 (Shewry et al, 1984).…”

Section: Introductionmentioning

confidence: 99%

Detection of the Sec-1 locus of rye by a PCR-based method.

1997

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The structural genes for the ω-secalins of rye (Secale cereale) are located in the Sec-1 locus on the short arm of rye chromosome 1R. We applied PCR (polymerase chain reaction) to detect the Sec-1 locus in a wheat genomic background. A primer set we designed based on a published sequence of a ω-secalin gene amplified not only the ω-secalin sequence, but also a putative ω-gliadin sequence. We determined partial sequences of both PCR-amplified fragments and designed different primers for the specific amplification of the ω-secalin sequence. One of the new primer sets amplified DNA fragments only in rye and wheat lines carrying chromosome 1R or telosome 1RS; no amplification occurred in either euploid wheats or 1RS deletion lines. This PCR-based method would provide efficient screening for the Sec-1 locus in progeny of wheat lines carrying chromosome 1R.

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“…These are the high molecular weight (HMW) secalins, ω-secalins, small γ-secalins (Mr=40000, 40 kDa), and large γ-secalins (Mr=75000, 75 kDa) (Shewry et al 1983a;Kasarda et al 1983).…”

Section: Discussionmentioning

confidence: 99%

Production and cytomolecular identification of new wheat-perennial rye (Secale cereanum) disomic addition lines with yellow rust resistance (6R) and increased arabinoxylan and protein content (1R, 4R, 6R)

et al. 2016

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Perennial rye (Secale cereanum, 2n=2x=14, RR) cultivar Kriszta has a large gene pool that can be exploited in wheat breeding. It has high protein and dietary fibre content, carries several resistance genes, tolerant to frost and drought, and adapts well to disadvantageous soil and weather conditions. In order to incorporate agronomically useful features from this perennial rye into cultivated wheat, backcross progenies derived from a cross between the wheat line Mv9kr1 and perennial rye 'Kriszta' have been produced, and addition lines disomic for 1R, 4R and 6R chromosomes have been selected using GISH, FISH and SSR markers. Quality measurements showed that addition of 'Kriszta' chromosomes 4R and 6R to the wheat genome had increased the total protein content. The 4R addition line contained slightly, while 1R and 6R additions significantly higher amount of arabinoxylan than the parental wheat line. Besides this, the 6R addition line appeared to be resistant to yellow rust in highly infected nurseries, consequently it may carry a new effective gene different from that harboured in the 1RS.1BL translocation for resistance to this disease.

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N-terminal amino acid sequences of ω-gliadins and ω-secalins

Cited by 200 publications

References 35 publications

The prolamin storage proteins of cereal seeds: structure and evolution

The prolamin storage proteins of cereal seeds: structure and evolution

Detection of the Sec-1 locus of rye by a PCR-based method.

Production and cytomolecular identification of new wheat-perennial rye (Secale cereanum) disomic addition lines with yellow rust resistance (6R) and increased arabinoxylan and protein content (1R, 4R, 6R)

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