N2‐Fixation in Field Beans Quantified by 15N Isotope Dilution. II. Effect of Cultivars of Beans1

Rennie, R. J.; Kemp, G. A.

doi:10.2134/agronj1983.00021962007500040016x

Cited by 108 publications

(66 citation statements)

References 5 publications

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“…However, the validity of indirect measures of N 2 -fixation such as SDW or N yield under field conditions is sometimes questionable . A previous study investigating genotypic differences in N 2 -fixing ability based on growth habit reported that climbing and indeterminate bean genotypes were superior in N 2 -fixation compared to most bush cultivars (Rennie and Kemp 1983). …”

Section: Resultsmentioning

confidence: 99%

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Biological nitrogen fixation and nifH gene expression in dry beans (Phaseolus vulgaris L.)

et al. 2014

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(1 ). To identify efficient N 2 fixers, 22 dry bean genotypes were screened for symbiotic N 2 -fixation in potted low-N soil in the greenhouse under four treatments: (1) (1 ) with either rhizobial inoculant, and Viva fixed the highest amount (129 mg N plant(1 ) when inoculated with Nitrastik-D. In contrast, AC Black Diamond, Island, Winchester, AC Polaris, LEF 2RB and SEA 5 had low N 2 -fixing potential (3 to 23 mg N plant(1 ) in inoculated treatments. The qRT-PCR analysis indicated that nifH gene expression was 5 fold higher in GH-196 compared to the non-nodulating mutant R99, which further confirmed the high N 2 -fixation capacity of GH-196. After confirmation in the field, dry bean genotypes with improved N 2 -fixation potential identified in this study may be used as parents in breeding programs in the development of future cultivars. The nifH gene expression may be used as a rapid test to select dry beans with high N 2 -fixation potential. L'analyse qRT-PCR re´ve`le que l'expression du ge`ne nifH est cinq fois plus e´leve´e chez GH-196 que chez l'autre mutant (R99) ne produisant pas de nodules, ce qui confirme encore plus la forte capacite´de fixation du premier. Apre`s confirmation au champ, les ge´notypes de haricot a`meilleur potentiel de fixation de l'azote identifie´s dans le cadre de cette e´tude pourraient servir de parents dans les programmes d'hybridation visant a`cre´er de nouveaux cultivars. L'expression du ge`ne nifH pourrait servir de test pour se´lectionner rapidement les varie´te´s de haricot qui fixent bien le N 2 .Mots clé s: Se´lection de ge´notypes, inoculation, fixation du N 2 , Rhizobium, RT-PCR 1 Corresponding author (e-mail: newton.lupwayi@agr. gc.ca).Abbreviations: RDW, root dry weight; RT-PCR, reverse transcription polymerase chain reaction; SDW, shoot dry weight Can.

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Section: Resultsmentioning

confidence: 99%

“…(1 (Rennie and Kemp 1983). However, in Saskatchewan, low amounts of N 2 fixed by 12 dry bean genotypes (16 to 27 kg ha…”

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Biological nitrogen fixation and nifH gene expression in dry beans (Phaseolus vulgaris L.)

et al. 2014

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“…Nevertheless, ranges of biologically fixed N 2 in fieldgrown bean lines were reported as from 25 to 65 kg ha -1 (Ruschel et al, 1982) or 40 to 125 kg ha -1 (Rennie & Kemp, 1983) in shoots at physiological maturity, or from 21 to 44 kg ha -1 in grains (Pereira et al, 1989). Additionally, field experiments in seven countries showed that the N 2 fixation contributed with 35 % of the N accumulated by bean crops, with the highest figures at 70 % (Hardarson et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Seeds enriched with phosphorus and molybdenum improve the contribution of biological nitrogen fixation to common bean as estimated by 15n isotope dilution

Chagas¹,

Araújo

Alves

et al. 2010

Rev. Bras. Ciênc. Solo

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SUMMARYSeeds with a high concentration of P or Mo can improve the growth and N accumulation of the common bean (Phaseolus vulgaris L.), but the effect of enriched seeds on biological N 2 fixation has not been established yet. This study aimed to evaluate the effect of seeds enriched with P and Mo on growth and biological N 2 fixation of the common bean by the 15 N isotope dilution technique. An experiment was carried out in pots in a 2 x 3 x 2 x 2 factorial design in randomized blocks with four replications, comprising two levels of soil applied P (0 and 80 mg kg -1 ), three N sources (without N, inoculated with rhizobia, and mineral N), two seed P concentrations (low and high), and two seed Mo concentrations (low and high). Non-nodulating bean and sorghum were used as non-fixing crops. The substrate was 5.0 kg of a Red Latosol (Oxisol) previously enriched with 15 N and mixed with 5.0 kg of sand. Plants were harvested 41 days after emergence. Seeds with high P concentration increased the growth and N in shoots, particularly in inoculated plants at lower applied P levels. Inoculated plants raised from high P seeds showed improved nodulation at both soil P levels. Higher soil P levels increased the percentage of N derived from the atmosphere (%Ndfa) in bean leaves. Inoculation with the selected strains increased the %Ndfa. High seed P increased the %Ndfa in inoculated plants at lower soil P levels. High seed Mo increased the %Ndfa at lower soil P levels in plants that did not receive inoculation or mineral N. It is concluded that high seed P concentration increases the growth, N accumulation RESUMO: SEMENTES ENRIQUECIDAS COM FÓSFORO E MOLIBDÊNIO

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“…Graham (1981) suggested that the ability to fix N was related to the supply of carbohydrates to the nodule, relative rates of N uptake from the soil and time to flowering and that type IV (indeterminate, climbing) plants fixed more N than type I plants that have a determinate, upright habit. Rennie and Kemp (1983b) have suggested that the bean genotype controls the length of the N 2 fixation period and thereby influences the total quantity of N fixed. In their study, ranking of dry bean cultivars for N 2 fixation was dependent on the Rhizobium strain, mineral N levels, growth habit of the cultivar, growth stage at sampling and temperature regime during evaluation.…”

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Response of four common bean cultivars to granular inoculant in a short-season dryland production system

Nleya¹,

Walley²,

Vandenberg³

2001

Can. J. Plant Sci.

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Nleya, T., Walley, F. and Vandenberg, A. 2001. Response of four common bean cultivars to granular inoculant in a shortseason dryland production system. Can. J. Plant Sci. 81: 385-390. Common bean (Phaseolus vulgaris L.) is considered to be a poor N fixer relative to other legumes. However, considerable genotypic variability between cultivars is known to exist. Determining the importance of the host genotype to the symbiotic process will assist breeders in developing bean cultivars with improved N 2 fixing capacity. Field experiments were conducted at two locations in Saskatchewan for two seasons to assess the N 2 fixation rates of four common bean cultivars differing in growth habit and maturity. Beans were inoculated using a granular inoculant applied in the seedrow or in a sideband, or not inoculated at all. Inoculation resulted in greater nodule dry mass, shoot dry mass, seed yield and amount of N derived from atmosphere as compared with the uninoculated control. The response to inoculant application was cultivar specific, with N derived from the atmosphere ranging from a low of 51% (CDC Camino) to a high of 78% (CDC Pinnacle). The cultivars responded similarly to inoculant placement. The sideband treatment was associated with a delay in maturity. This may limit the suitability of this treatment where early maturity of the crop is critical. En précisant l'importance du génotype de l'hôte dans la symbiose, on aidera les obtenteurs à créer des cultivars fixant mieux l'azote. Les auteurs ont entrepris des essais sur le terrain à deux endroits, en Saskatchewan, pendant deux saisons afin d'établir le taux de fixation d'azote de quatre cultivars de haricot, différents au niveau du port et de la maturité. Un inoculant granulaire a été appliqué avec la graine ou à côté de celle-ci. D'autres plants n'ont reçu aucun inoculant. L'inoculation augmente la masse sèche des nodules, la masse sèche des pousses, le rendement grainier et la quantité d'azote tirée de l'atmosphère comparativement aux témoins qui n'avaient pas reçu d'inoculant. La réaction à l'inoculant dépend du cultivar, la quantité de N extraite de l'atmosphère variant d'un minimum de 51 % (CDC Camino) à un maximum de 78 % (CDC Pinnacle). Les cultivars réagissent de la même façon à l'inoculant. L'application latérale d'inoculant retarde néanmoins la maturité physiologique. Il se pourrait que cette propriété limite l'utilité d'un tel traitement quand la précocité de la culture joue un rôle capital.

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N₂‐Fixation in Field Beans Quantified by ₁₅N Isotope Dilution. II. Effect of Cultivars of Beans¹

Cited by 108 publications

References 5 publications

Biological nitrogen fixation and nifH gene expression in dry beans (Phaseolus vulgaris L.)

Biological nitrogen fixation and nifH gene expression in dry beans (Phaseolus vulgaris L.)

Seeds enriched with phosphorus and molybdenum improve the contribution of biological nitrogen fixation to common bean as estimated by 15n isotope dilution

Response of four common bean cultivars to granular inoculant in a short-season dryland production system

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