N,N′-Carbonyldiimidazole-mediated functionalization of superparamagnetic nanoparticles as vaccine carrier

Ho, Jenny; Al-Deen, Fatin Muhammed Nawwab; Al-Abboodi, Aswan; Selomulya, Cordelia; Xiang, Sue D.; Forde, Gareth M.

doi:10.1016/j.colsurfb.2010.11.001

Cited by 34 publications

(18 citation statements)

References 27 publications

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“…MNPs in the viral size have high intracellular uptake, whereas CD4 and CD8T cells and antibodies could be elicited when antigen is incorporated on the NPs surfaces. These properties of MNPs make them advantageous to previous applied methods [36]. The advantages of MNPs for treating respiratory diseases relay on the fact that nanoaerosols carriers can reduce the amount of the drug deposited into the lungs, avoid biological metabolism and deposition in the liver, penetrate into the lungs and reach alveoli due to their small size, target specific tissues and also having the possibility of being imaged using nano-optical techniques [37].…”

Section: Magnetite Nanoparticles Used In Biomedical Applicationsmentioning

confidence: 99%

Magnetite Nanostructures as Novel Strategies for Anti-Infectious Therapy

2014

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Abstract:This review highlights the current situation of antimicrobial resistance and the use of magnetic nanoparticles (MNPs) in developing novel routes for fighting infectious diseases. The most important two directions developed recently are: (i) improved delivery of antimicrobial compounds based on a drastic decrease of the minimal inhibition concentration (MIC) of the drug used independently; and (ii) inhibition of microbial attachment and biofilm development on coated medical surfaces. These new directions represent promising alternatives in the development of new strategies to eradicate and prevent microbial infections that involve resistant and biofilm-embedded bacteria. Recent promising applications of MNPs, as the development of delivery nanocarriers and improved nanovehicles for the therapy of different diseases are discussed, together with the mechanisms of microbial inhibition.

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Section: Magnetite Nanoparticles Used In Biomedical Applicationsmentioning

confidence: 99%

Magnetite Nanostructures as Novel Strategies for Anti-Infectious Therapy

2014

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“…The active intermediate formed by the reaction of the CDI with the hydroxyl group is an imidazolyl carbonyl. 26 Therefore, a mHAP with an active hydroxyl group could theoretically be coupled to the diamine groups of PEG resulting in a one-carbon space distance, which would form a stable urethane (N -alkyl carbamate) linkage. 27 Subsequently, the carboxylic acid of the HA reacts with EDAC/ NHS to form an acyl amino ester that then reacts with the primary amine of the PEG-modified mHAP to create a stable amide bond (Scheme 1).…”

Section: Resultsmentioning

confidence: 99%

Targeted Delivery of Hyaluronan-Immobilized Magnetic Ceramic Nanocrystals

Wu¹,

Wang²,

Hsieh³

et al. 2016

j biomed nanotechnol

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Effective cancer therapy relies on delivering the therapeutic agent precisely to the target site to improve the treatment outcome and to minimize side effects. Although surgery, chemotherapy, and radiotherapy are the standard methods commonly used in clinics, hyperthermia has been developed as a new and promising strategy for cancer therapy. In this study, magnetic bioceramic hydroxyapatite (mHAP) nanocrystals have been developed as heat mediator for intracellular hyperthermia. Hyaluronic acid (HA) modified mHAP nanocrystals are synthesized by a wet chemical precipitation process to achieve active targeting. The results demonstrate that the HA targeting moiety conjugated by a poly(ethylene glycol) (PEG) spacer arm is successfully immobilized on the surface of mHAP. The HA-modified mHAP possesses relatively good biocompatibility, an adequate biodegradation rate and superparamagnetic properties. The HA-modified mHAP could be localized and internalized into HA receptor-overexpressed malignant cells (e.g., MDA-MB-231 cell) and used as the heat generating agent for intracellular hyperthermia. The results from this study indicate that biocompatible HA-modified mHAP shows promise as a novel heat mediator and a specific targeting nanoagent for intracellular hyperthermia cancer therapy.

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“…CDI-agarose was prepared by modifying the protocol described in literature. [46,47] Sepharose TM CL-6B (agarose, 3 g) was suspended in dry acetone (50 mL) and CDI (5 g) was added. The mixture was kept under stirring overnight at room temperature.…”

Section: Preparation Of Agarose Based Carriersmentioning

confidence: 99%

Immobilization of γ‐Glutamyl Transpeptidase from Equine Kidney for the Synthesis of kokumi Compounds

et al. 2019

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γ‐Glutamyl transpeptidase from equine kidney (ekGGT, E.C. 2.3.2.2) is an intrinsic membrane enzyme which transfers the γ‐glutamyl moiety of glutathione to amino acids and peptides, thus producing γ‐glutamyl derivatives. An immobilization study of ekGGT was carried out with the aim to develop a robust biocatalyst for the synthesis of γ‐glutamyl amino acids which are known as kokumi compounds. Heterofunctional octyl‐glyoxyl‐agarose resulted in a high immobilization yield and activity recovery (93 % and 88 %, respectively). Immobilized ekGGT retained more than 95 % activity under reaction conditions (Tris‐HCl, pH 9, 0.05 M) after 6 days, whereas the residual activity after 6 reaction cycles (18 days) was 85 %. The synthesis of γ‐glutamylmethionine catalyzed by octyl‐glyoxyl‐agarose‐ekGGT afforded the product in 42 % yield (101 mg). The immobilized ekGGT was characterized by Raman spectroscopy. The immobilization protocol developed for ekGGT could be of general applicability to membrane proteins.

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N,N′-Carbonyldiimidazole-mediated functionalization of superparamagnetic nanoparticles as vaccine carrier

Cited by 34 publications

References 27 publications

Magnetite Nanostructures as Novel Strategies for Anti-Infectious Therapy

Magnetite Nanostructures as Novel Strategies for Anti-Infectious Therapy

Targeted Delivery of Hyaluronan-Immobilized Magnetic Ceramic Nanocrystals

Immobilization of γ‐Glutamyl Transpeptidase from Equine Kidney for the Synthesis of kokumi Compounds

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