N-Methylmelamines, a Unique Class of Anti-Tumour Agents?

Functions of Essential Genes and a Scale-Free Protein Interaction Network Revealed by Structure-Based Function and Interaction Prediction for a Minimal Genome

“…Studies based on transposon knockout libraries or highthroughput targeted deletions substantially enhanced our ability to rationally design reduced genomes by providing a high-throughput approach for identifying all genes that could not be individually knocked out. [6][7][8][9][10][11][12] Such knockout libraries cannot, however, provide all needed information for construction of a minimal genome because of the presence of both positive and negative epistatic interactions that cannot be captured in a single pass using such approaches. 3,7 More targeted work 13 provided a window into the overall reducibility of microbial genomes by deleting all prophages and mobile genetic elements from Escherichia coli MG1655, yielding a genome that was reduced in size by ~15%; the reduced genome strain, MDS42, also showed several useful properties such as increased stability of cloned genes.…”