N-methyl-N′-nitro-N-nitrosoguanidine mutagenesis and lethality in Aerobacter (Klebsiella) aerogenes

Beach, Callum; Dean, A. C. R.

doi:10.1007/bf00334237

Molec. Gen. Genetics

1969

DOI: 10.1007/bf00334237

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N-methyl-N′-nitro-N-nitrosoguanidine mutagenesis and lethality in Aerobacter (Klebsiella) aerogenes

Callum Beach

A. C. R. Dean

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1971

1976

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Cited by 9 publications

(1 citation statement)

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“…Induction of mutations with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) was carried out in 50 mM tris(hydroxymethyl)aminomethane, 50 mM maleic acid, pH 6.0, as previously described (1,2). Standard exposure conditions were 0.1 mg of MNNG per ml for 30 min at 37 C. Survivors were diluted 1:20 into B broth and grown overnight.…”

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Construction of intergeneric hybrids using bacteriophage P1CM: transfer of the Klebsiella aerogenes ribitol dehydrogenase gene to Escherichia coli

Rigby¹,

Gething²,

Hartley³

1976

J Bacteriol

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Study of many of the interesting properties of Klebsiella aerogenes is limited by the lack of a well-characterized genetic system for this organism. Our investigations of the evolution of the enzyme ribitol dehydrogenasel?EC -1.1.1.56) in K. aerogenes would be greatly facilitated by the availability of such a system, and we here report two approaches to developing one. We have isolated mutants sensitive to the coliphage P1, which will efficiently transduce genetic markers between such sensitive strains and which will thus make detailed mapping studies possible. Derivatives of K. aerogenes lysogenic for P1 can be readily isolated by using the specialized transducing particle PlCMclrlOO. Bacteria lysogenic for this phage are chloramphenicol resistant and temperature sensitive. Phage particles produced by temperature induction of such lysogens can be used to transfer K. aerogenes genes to the natural host of P1 phage, Escherichia coli. We have used this method to prepare derivatives of E. coli K-12 carrying the K. aerogenes genes conferring the ability to metabolize the pentitols ribitol and D-arabitol. We have shown that these E. coli-K. aerogenes hybrids synthesize a ribitol dehydrogenase with the properties of the K. aerogenes enzyme and have mapped the position of the transferred gene on the E. coli chromosome. The ramifications of this methodology are discussed.

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confidence: 99%

Construction of intergeneric hybrids using bacteriophage P1CM: transfer of the Klebsiella aerogenes ribitol dehydrogenase gene to Escherichia coli

Rigby¹,

Gething²,

Hartley³

1976

J Bacteriol

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The isolation and characterization of auxotrophs of the aspartic acid family fromStreptomyces lipmanii

Kirkpatrick

Godfrey

1973

Folia Microbiol

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Fysiologické a mutagenní účinky N-methyl-N′-nitro-N-nitrosoguanidinu (MNNG) na populace chlorokokálních řas

Nečas

1975

Biol Plant

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N-methyl-N′-nitro-N-nitrosoguanidine mutagenesis and lethality in Aerobacter (Klebsiella) aerogenes

Cited by 9 publications

References 10 publications

Construction of intergeneric hybrids using bacteriophage P1CM: transfer of the Klebsiella aerogenes ribitol dehydrogenase gene to Escherichia coli

Construction of intergeneric hybrids using bacteriophage P1CM: transfer of the Klebsiella aerogenes ribitol dehydrogenase gene to Escherichia coli

The isolation and characterization of auxotrophs of the aspartic acid family fromStreptomyces lipmanii

Fysiologické a mutagenní účinky N-methyl-N′-nitro-N-nitrosoguanidinu (MNNG) na populace chlorokokálních řas

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