N-Methyl- D -aspartic acid- and metaphit-induced audiogenic seizures in rat model of seizures

Stanojlović, Olivera; Živanović, Dubravka; Šušić, Veselinka

doi:10.1006/phrs.2000.0685

Cited by 25 publications

(21 citation statements)

References 27 publications

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“…), placed in a stereotaxic apparatus, and three gold-plated recording electrodes were implanted over frontal, parietal, and occipital cortices. An EEG apparatus (RIZ, Zagreb, Croatia) with digital signal acquisition and corresponding software (NeurosciLaBG) was used (as described in Stanojlović et al 2000Stanojlović et al , 2007. The frequency range was defined by the time constant (0.3 s), lower and upper limit frequencies (0.5 and 100 Hz, respectively) further digitized at a sample rate of 512 Hz.…”

Section: Eeg/swd Recordingsmentioning

confidence: 99%

Two Types of Seizures in Homocysteine Thiolactone-Treated Adult Rats, Behavioral and Electroencephalographic Study

et al. 2008

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D,L-homocysteine thiolactone (H), a reactive homocysteine metabolite, contributes to total homocysteine pool. The aim of the present study was to determine the effects of H after acute application in increasing doses to rats. Adult Wistar rat were intraperitoneally administered saline or H in increasing doses (5.5, 8.0, or 11.0 mmol/kg). For electroencephalographic (EEG) recordings, three gold-plated screws were implanted into the skull and animals were supervised. We observed H-induced two types of seizures, the coexistence of convulsive and nonconvulsive epilepsy. Dose-related increase in the number and severity (0-4) of displaying convulsions was recorded. In H(5.5) group, the majority of seizure episodes were grade 1 (62.5 and 0% lethality), in H(8) 40% grade 2, and in H(11) grade 4 in 42.11% (100% lethal outcome). EEGs recordings in convulsive animals showed a high-voltage spike-wave and polyspikes complexes. The second, absence-like, nonconvulsive seizures were accompanied by the EEGs mostly with 6-8 Hz spikes-and-wave discharges (SWD). Latency time to the generalized clonic-tonic seizures overlapped with the time of the maximal median number and median duration of the SWD per 15 min during 90-min observing period. The results show that acute H administration significantly changes neurons, EEG tracings, and behavioral responses and suggests a possible model for studying petit mal epilepsy.

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Section: Eeg/swd Recordingsmentioning

confidence: 99%

Two Types of Seizures in Homocysteine Thiolactone-Treated Adult Rats, Behavioral and Electroencephalographic Study

et al. 2008

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“…Lipovac et al [14] suggested an overaccumulation of extracellular glutamate levels, as a result of inhibition of the transport system for glutamate clearance and glial uptake inhibition in the brain by metaphit. All above mentioned events could be the reason for brain hyperexcitability, evident as intense spiking activity on AGS, as a trigger factor, recorded in EEGs in the present and earlier studies [16,17] . DSIP and VPA are quite different by their chemical structure and pharmacokinetic profiles, but some resemblance persists in their anticonvulsive activity.…”

Section: Discussionmentioning

confidence: 88%

“…in a dose of 10 mg/kg. Eight h after metaphit administration, when the convulsive activity was fully developed [17] , the animals received i.p. VPA (50 mg/kg), DSIP (1.0 mg/kg) or their combination.…”

Section: Study Design and Experimental Proceduresmentioning

confidence: 99%

Delta-Sleep-Inducing Peptide Potentiates Anticonvulsive Activity of Valproate against Metaphit-Provoked Audiogenic Seizure in Rats

et al. 2006

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The effect of delta-sleep-inducing peptide (DSIP) on the anticonvulsive activity of a nonprotective valproate (VPA) dose in a metaphit model of generalized, reflex audiogenic seizures in adult Wistar rats was studied. The animals that received metaphit (10 mg/kg) were exposed to audiogenic stimulation (100 ± 3 dB, 60 s) at hourly intervals. Metaphit-treated rats displaying seizures in 8 previous tests were i.p. injected with VPA (50 mg/kg) or DSIP (1.0 mg/kg) or their combination. Latency to seizure was behaviorally assessed. The EEGs and power spectra were recorded and analyzed. Neurotoxicity was evaluated by the chimney test. DSIP or VPA alone expressed no significant effect on the latency duration, but their combination significantly prolonged latency to seizure during 6 h after injection, while inducing no significant motor impairment. Neither the applied drugs nor their combination abolished metaphit-provoked EEG epileptiform activity. The results show that DSIP potentiated anticonvulsive effects of a nonprotective VPA dose in a metaphit model of audiogenic seizures without influencing its neurotoxicity.

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“…Experimental models of generalised clonic-tonic seizures induced by metaphit (1-[1(3-isothiocyanatophenyl)-cyclohexyl] piperidine) (24,25) and lindane (γ-1,2,3,4,5,6-hexachlorocyclohexane) (26,27) are widely used for the study of epilepsy and the preclinical evaluation of potential antiepileptic treatments. In contrast to audiogenic epilepsy induced by metaphit with its typical and characteristic three degrees of intensity, the lindane model is similar to Hcy-induced epilepsy with a wide spectra of behavioural manifestations (e.g., hot plate reaction and Kangaroo position) incorporated into the rating scale from grades 0 -4.…”

Section: Homocysteine and Seizuresmentioning

confidence: 99%

Homocysteine: Neurotoxicity and mechanisms of induced hyperexcitability

Stanojlović¹,

Hrnčić²,

Rašić-Marković³

et al. 2011

Ser J Exp Clin Res

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N-Methyl- D -aspartic acid- and metaphit-induced audiogenic seizures in rat model of seizures

Cited by 25 publications

References 27 publications

Two Types of Seizures in Homocysteine Thiolactone-Treated Adult Rats, Behavioral and Electroencephalographic Study

Two Types of Seizures in Homocysteine Thiolactone-Treated Adult Rats, Behavioral and Electroencephalographic Study

Delta-Sleep-Inducing Peptide Potentiates Anticonvulsive Activity of Valproate against Metaphit-Provoked Audiogenic Seizure in Rats

Homocysteine: Neurotoxicity and mechanisms of induced hyperexcitability

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