N-linked glycosylation of β-amyloid precursor protein

Interleukin‐1β dissociates β‐amyloid precursor protein and β‐amyloid peptide secretion

“…The secretion seems transcriptionally-independent since the transcription of the j?APP gene (> 400 kB) and the subsequent translation of the message (lOO-120 kDa) require more than 1 h as suggested by others [18,19]. Our results are consistent witH those of Buxbaum et al who attributed enhanced secretion of j3APP by IL-l/3 to metabolic changes rather than to changes in transcriptional rate [lo].…”

Zinc Inhibits Amyloid β Production from Alzheimer's Amyloid Precursor Protein in SH-SY5Y Cells

“…APP is known to be detected as multiple bands on immunoblotting when labeled with an APP C-terminus antibody. The immature form has been reported as 91∼106 kD and the mature form as 20 kD larger (Weidemann et al, 1989;Caporaso et al, 1992;Pahlsson et al, 1992). Tomita et al (1998) reported immature APP as two bands, results consistent with our findings (Fig.…”

“…The immature form of APP is N-glycosylated and the mature form is N-and O-glycosylated (Weidemann et al, 1989). This maturation is considered to be a prerequisite for Aβ production and leads to an increase in molecular mass of 19∼22 kilodaltons (kD) (Weidemann et al, 1989;Pahlsson et al, 1992;Tomita et al, 1998). Any factors that accelerate Aβ aggregation can be precipitating factors in the development of AD, including enhanced production of Aβ, a decreased Aβ clearance rate and the promotion of Aβ precipitation (Bush et al, 1994).…”

SorLA Complement-type Repeat Domains Protect the Amyloid Precursor Protein against Processing

“…In many cases, post-translation modifications such as glycosylation are important for regulating the intracellular sorting processes and stabilizing against proteolysis (70). Glycosylation, particularly sialylation, of APP has been proposed to influence the processing pathway of APP by determining its passage from the Golgi to the cell membrane (50,71,72).…”