N-Hydroxysuccinimide-terminated self-assembled monolayers on gold for biomolecules immobilisation

Cabrita, Joana F.; Abrantes, L.M.; Viana, Ana S.

doi:10.1016/j.electacta.2004.09.019

Cited by 83 publications

(51 citation statements)

References 27 publications

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“…However, there is a discernible shift in the peak potential of the major peak towards more negative values when the concentration of DSP is reduced from 1.0 mM to 0.1 mM. The peak potential is indicative of the permeability of the solvent into the monolayer [49]. When the monolayer is prepared in solution,…”

Section: Characterization Of Self-assembled Monolayermentioning

confidence: 99%

Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure

Suherman

Morita²,

Kawaguchi

2015

Applied Surface Science

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Surface plasmon resonance sensor equipped with a fabricated immunosensor chip is used for detecting clenbuterol in this study. Since clenbuterol is a small analyte, indirect competitive inhibition immunoassay is employed. For fabricating the immunosurface, the Au-chip was functionalized by succinimidyl-terminated alkanethiol, and the terminal N-hydroxysuccinimide group of the self-assembled monolayer was either replaced with clenbuterol or blocked with ethanolamine. Scanning tunneling microscope experiments and electrochemical measurements depicted the domain structures of the succinimide group of succinimidyl-terminated propanethiol monolayer. The surface concentration and the orientation of succinimide group was significantly dependent on the concentration of dithiobis(succinimidyl) propionate (DSP) used in fabricating the monolayer. Furthermore, the structure of monolayer significantly influenced both the surface concentration and the orientation of clenbuterol on the sensor surface. Consequently, high coverage and standing-up configuration of clenbuterol showed high affinity for clenbuterol antibody. However, high affinity constant exhibited by the sensor surface was coupled with a low sensitivity. By contrast, lowest concentration of DSP solution (0.1 mM) used in fabricating the immunosurface showed a detection sensitivity of 3 ppt-the highest reported sensitivity for clenbuterol. For regeneration the immunosurface, 0.1 M NaOH was used and the same sensor surface could be reused for performing >100 rapid immunoreaction.

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Section: Characterization Of Self-assembled Monolayermentioning

confidence: 99%

Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure

Suherman

Morita²,

Kawaguchi

2015

Applied Surface Science

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“…Electrochemical reductive desorption is one of the most used methodologies to characterize modified surfaces with self-assembled monolayers [31][32][33]. The cyclic voltammograms recorded at 20 mV s − 1 in 0.5 M NaOH of the different modified electrodes under study are depicted in Fig.…”

Section: Cyclic Voltammetrymentioning

confidence: 99%

Immunosensor interface based on physical and chemical immunoglobulin G adsorption onto mixed self-assembled monolayers

Wang

Viana

Jin

et al. 2006

Bioelectrochemistry

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An immunosensor interface based on mixed hydrophobic self-assembled monolayers (SAMs) of methyl and carboxylic acid terminated thiols with covalently attached human Immunoglobulin G (hIgG), is investigated. The densely packed and organised SAMs were characterised by contact angle measurements and cyclic voltammetry. The effect of the non-ionic surfactant, Tween 20, in preventing nonspecific adsorption is addressed by ellipsometry during physical and covalent hIgG immobilization on pure and mixed SAMs, respectively. It is clearly demonstrated that nonspecific adsorption due to hydrophobic interactions of hIgG on methyl ended groups is totally inhibited, whereas electrostatic/hydrogen bonding interactions with the exposed carboxylic groups prevail in the presence of surfactant. Results of ellipsometry and Atomic Force Microscopy, reveal that the surface concentration of covalently immobilized hIgG is determined by the ratio of COOH / CH 3 -terminated thiols in SAM forming solution. Moreover, the ellipsometric data demonstrates that the ratio of bound anti-hIgG / hIgG depends on the density of hIgG on the surface and that the highest ratio is close to three. We also report the selectivity and high sensitivity achieved by chronoamperometry in the detection of adsorbed hIgG and the reaction with its antibody.

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“…An amine substitute of DNT, 4-(2,4-dinitrophenyl)butan-1-amine, was Immobilization of a DNT derivative on a gold surface with biopanning. Synthesized 4-(2,4-dinitrophenyl)butan-1-amine was immobilized on a gold-coated surface self-assembled monolayer containing an amine reactive group as previously described (11). Briefly, the gold-coated surface was washed with ethanol and reacted with 2 mM of 2,5-dioxopyrrolidin-1-yl 23-mercapto-3,6,9,12-tetraoxatricosan-1-oate in ethanol for 14 hr at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Identification of Dinitrotoluene Selective Peptides by Phage Display Cloning

Jang¹,

Na²,

Jin³

et al. 2010

Bulletin of the Korean Chemical Society

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Biomolecules specific to explosives can be exploited as chemical sensors. Peptides specific to immobilized dinitrotoluene (DNT) were identified using a phage display library. A derivative of DNT that contained an extended amine group, 4-(2,4-dinitrophenyl)butan-1-amine, was synthesized and immobilized using a self-assembled monolayer surface on gold. Filamentous M13 phages displaying random sequences of 12-mer peptides specific to the immobilized DNTderivate were isolated from the M13 phage library by biopanning. A common peptide sequence was identified from the isolated phages and the synthesized peptides showed selective binding to DNT. When the peptide was immobilized on a quartz crystal microbalance (QCM) chip, it showed a binding signal to DNT, while toluene barely showed significant binding to the QCM chip. These results demonstrate that peptides screened by biopanning against immobilized DNT can be useful for quick and accurate detection of DNT.

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N-Hydroxysuccinimide-terminated self-assembled monolayers on gold for biomolecules immobilisation

Cited by 83 publications

References 27 publications

Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure

Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure

Immunosensor interface based on physical and chemical immunoglobulin G adsorption onto mixed self-assembled monolayers

Identification of Dinitrotoluene Selective Peptides by Phage Display Cloning

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