N-Glycosylation of secretion enhancer peptide as influencing factor for the secretion of target proteins from Saccharomyces cerevisiae

Han, Kyoung Sim; Kim, Seo Il; Choi, Seong Il; Seong, Baik Lin

doi:10.1016/j.bbrc.2005.09.089

Cited by 6 publications

(5 citation statements)

References 24 publications

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“…N ‐glycosylation of the propeptide derived from hIL‐1β, which occurs at a specific “Asn” site, has been found to play an important role in the secretion of heterologous proteins (K. S. Han et al, 2005 ; M. Han et al, 2014 ). N ‐glycosylation plays a significant role in the secretion of hG‐CSF (K. S. Han et al, 2005 ), presumably because of the enhanced folding of rhG‐CSF in the ER (Doyon et al, 2002 ; Sagt et al, 2000 ), and is subsequently involved in the transition of rhG‐CSF from the ER to the Golgi apparatus (Ari et al, 2001 ).…”

Section: Resultsmentioning

confidence: 99%

“…Finally, the % increased efficiency of rhG-CSF secretion was estimated as the ratio of the concentration of mature rhG-CSF from the best pro-peptide sample in each stage to the concentration of mature rhG-CSF from the original pro-peptide. of hG-CSF (K. S. Han et al, 2005), presumably because of the enhanced folding of rhG-CSF in the ER (Doyon et al, 2002;Sagt et al, 2000), and is subsequently involved in the transition of rhG-CSF from the ER to the Golgi apparatus (Ari et al, 2001).…”

Section: Design Of Secretion Enhancing Peptide Cassette For Heterolog...mentioning

confidence: 99%

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Synthetic pro‐peptide design to enhance the secretion of heterologous proteins by Saccharomyces cerevisiae

Cho

Jang

et al. 2022

MicrobiologyOpen

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Heterologous protein production in Saccharomyces cerevisiae is a useful and effective strategy with many advantages, including the secretion of proteins that require posttranslational processing. However, heterologous proteins in S. cerevisiae are often secreted at comparatively low levels. To improve the production of the heterologous protein, human granulocyte colony‐stimulating factor (hG‐CSF) in S. cerevisiae , a secretion‐enhancing peptide cassette including an hIL‐1β‐derived propeptide, was added and used as a secretion enhancer to alleviate specific bottlenecks in the yeast secretory pathway. The effects of three key parameters— N ‐glycosylation, net negative charge balance, and glycine‐rich flexible linker—were investigated in batch cultures of S. cerevisiae . Using a three‐stage design involving screening, selection, and optimization, the production and secretion of hG‐CSF by S. cerevisiae were significantly increased. The amount of extracellular mature hG‐CSF produced by the optimized propeptide after the final stage increased by 190% compared to that of the original propeptide. Although hG‐CSF was used as the model protein in the current study, this strategy applies to the enhanced production of other heterologous proteins, using S. cerevisiae as the host.

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Section: Resultsmentioning

confidence: 99%

Section: Design Of Secretion Enhancing Peptide Cassette For Heterolog...mentioning

confidence: 99%

Synthetic pro‐peptide design to enhance the secretion of heterologous proteins by Saccharomyces cerevisiae

Cho

Jang

et al. 2022

MicrobiologyOpen

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“…Many IL1 family members lack classical signal sequences necessary for secretion via the ER-Golgi complex pathway. These cytokines are released by autophagy, necrotic cells, or poorly defined nonclassical secretion pathways (34). No changes in the expression of the autophagy markers LC3-I, LC3-II, and p62 were observed after treatment with bleomycin (data not shown).…”

Section: Discussionmentioning

confidence: 98%

Apoptotic Cells Release IL1 Receptor Antagonist in Response to Genotoxic Stress

Chwee

Khatoo

Tan

et al. 2016

Cancer Immunology Research

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Apoptosis is a controlled means of eliminating damaged cells without causing an inflammatory response or tissue damage. The mechanisms that contribute to the suppression of an inflammatory response upon apoptosis of cells are poorly understood. Here, we report that apoptotic cells release the interleukin-1 receptor antagonist (IL1RA). The release of IL1RA depended on the DNA damage response, caspase 9, and caspase 3. De novo translation, classical secretion pathways, or N-glycosylation was not required for the release of IL1RA. The amounts of IL1RA released by apoptotic cells impaired IL1-induced expression of IL6. In summary, we demonstrate that the release of IL1RA in response to genotoxic stress contributes to the immunosuppressive effects of apoptotic cells.

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“…To enhance the quality and quantity of yeast-based VLPs, various factors, such as the type of plasmid and promoter and, especially the secretability and processing on exit out of the yeast cells, need to be carefully taken into account (Bae et al, 1998;Lee et al, 1999;Han et al, 2005). The baculovirus-insect cell and mammalian-cell systems have an advantage of eliciting more complete post-translational modification including glycosylation and expressing multiple component VLPs (Rodriguez-Limas et al, 2011).…”

Section: Expression Platforms For Vlpsmentioning

confidence: 99%

Exploiting virus-like particles as innovative vaccines against emerging viral infections

Jeong

Seong

2017

J Microbiol.

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Emerging viruses pose a major threat to humans and livestock with global public health and economic burdens. Vaccination remains an effective tool to reduce this threat, and yet, the conventional cell culture often fails to produce sufficient vaccine dose. As an alternative to cell-culture based vaccine, virus-like particles (VLPs) are considered as a highpriority vaccine strategy against emerging viruses. VLPs represent highly ordered repetitive structures via macromolecular assemblies of viral proteins. The particulate nature allows efficient uptake into antigen presenting cells stimulating both innate and adaptive immune responses towards enhanced vaccine efficacy. Increasing research activity and translation opportunity necessitate the advances in the design of VLPs and new bioprocessing modalities for efficient and cost-effective production. Herein, we describe major achievements and challenges in this endeavor, with respect to designing strategies to harnessing the immunogenic potential, production platforms, downstream processes, and some exemplary cases in developing VLP-based vaccines.

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N-Glycosylation of secretion enhancer peptide as influencing factor for the secretion of target proteins from Saccharomyces cerevisiae

Cited by 6 publications

References 24 publications

Synthetic pro‐peptide design to enhance the secretion of heterologous proteins by Saccharomyces cerevisiae

Synthetic pro‐peptide design to enhance the secretion of heterologous proteins by Saccharomyces cerevisiae

Apoptotic Cells Release IL1 Receptor Antagonist in Response to Genotoxic Stress

Exploiting virus-like particles as innovative vaccines against emerging viral infections

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