N-glycoprotein profiling of lung adenocarcinoma pleural effusions by shotgun proteomics

Soltermann, Alex; Ossola, Reto; Kilgus-Hawelski, Sandra; Eckardstein, Arnold von; Suter, Tobias; Aebersold, Ruedi; Moch, Holger

doi:10.1002/cncr.23349

Cited by 54 publications

(51 citation statements)

References 29 publications

(28 reference statements)

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“…The association of TF with two identified ECM proteins, periostin and versican, was validated by IHC. Periostin is a secreted ECM protein is observed in the desmoplastic stroma of a variety of cancers and known to promote cell invasion (25)(26)(27)(28). It is also involved in cardiac remodeling after myocardial infarction (29).…”

Section: Discussionmentioning

confidence: 99%

Morphoproteomic Characterization of Lung Squamous Cell Carcinoma Fragmentation, a Histological Marker of Increased Tumor Invasiveness

et al. 2017

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Section: Discussionmentioning

confidence: 99%

Morphoproteomic Characterization of Lung Squamous Cell Carcinoma Fragmentation, a Histological Marker of Increased Tumor Invasiveness

et al. 2017

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“…We have identified previously the EMT indicator protein periostin in malignant pleural effusions from lung adenocarcinoma by mass spectrometric analysis (19). This finding prompted us to investigate this protein in a larger cohort of NSCLC by immunohistochemistry.…”

Section: Discussionmentioning

confidence: 98%

Prognostic Significance of Epithelial-Mesenchymal and Mesenchymal-Epithelial Transition Protein Expression in Non–Small Cell Lung Cancer

Soltermann¹,

Tischler²,

Arbogast³

et al. 2008

Clinical Cancer Research

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Purpose: In carcinomas, invasive tumor growth is accompanied by desmoplastic stroma reaction and facilitated by epithelial-mesenchymal transition (EMT) of cancer cells. We investigated the prognostic significance of the EMT indicator proteins periostin and vimentin in comparison with versican, a putative indicator of the opposite mechanism mesenchymal-epithelial transition (MET), and to the desmoplasia proteins collagen and elastin in non-small cell lung cancer (NSCLC). Experimental Design: Tumor of 533 patients with surgically resected NSCLC was used for analysis of stromal and epithelial protein expression by immunohistochemistry (EMT-MET proteins) and Elastica van Gieson histochemical staining (collagen and elastin). A semiquantitative sum scoring system was done on three tissue microarrays. Results: Of the 533 patients, 48% had squamous cell carcinoma, 47% adenocarcinoma, and 5% adenosquamous carcinoma. High expression of periostin in either stroma or tumor epithelia, independently scored by two pathologists, correlated with male gender, higher stage, higher pT category, and larger tumor size, and in only stroma with tumor relapse. High expression of versican in either stroma or epithelia as well as of stromal collagen had fewer but concordant associations with advanced tumor and periostin, respectively. High expression of elastin was oppositely associated with less advanced disease. Associations of high vimentin were inconsistent (all P values <0.05). High stromal periostin was found to be a prognostic factor for decreased progression-free survival on univariate analysis (P = 0.007). Conclusions: Because up-regulation is frequently observed in the stromal and epithelial tumor compartment, EMT-MET indicator proteins may be integrated in progression models of NSCLC.

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“…Several proteomics techniques have been applied previously to detect markers of lung cancer using plasma and pleural effusion as starting material [10][11][12][13][14][15]. However, as of today, no biomarkers have reached the clinic.…”

Section: Introductionmentioning

confidence: 99%

Use of narrow‐range peptide IEF to improve detection of lung adenocarcinoma markers in plasma and pleural effusion

et al. 2009

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In this study we applied narrow-range peptide IEF to plasma or pleural effusion prior to LC/MS/MS. Two methods for narrow-range IEF were run; IPG strips and free-flow electrophoresis. Data from this study was compared with cell line data to evaluate the method performance in body fluids. To test the methods potential in quantitative biomarker discovery studies, plasma and pleural effusion from patients with lung adenocarcinoma (n 5 3) were compared with inflammatory pleuritis (n 5 3) using iTRAQ quantification. Using narrow-range IEF on the peptide level we were able to identify and quantify 282 proteins in plasma and 300 proteins in pleural effusion. These body fluid proteomes demonstrated high degree of overlap; however, more proteins significantly differently altered levels related to adenenocarcinoma were found in pleural effusion compared with plasma, suggesting enrichment of lung tissue-related proteins in pleural effusion. Nine proteins were chosen for initial validation with Western blot, and one protein (NPC2) was chosen for further validation using imunohistochemistry. Overall, the quantitative results from IEF/LC/MS/MS showed good correlation with the results from Western blot and imunohistochemistry, showing the potential of this methodology in quantitative biomarker discovery studies.

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N-glycoprotein profiling of lung adenocarcinoma pleural effusions by shotgun proteomics

Cited by 54 publications

References 29 publications

Morphoproteomic Characterization of Lung Squamous Cell Carcinoma Fragmentation, a Histological Marker of Increased Tumor Invasiveness

Morphoproteomic Characterization of Lung Squamous Cell Carcinoma Fragmentation, a Histological Marker of Increased Tumor Invasiveness

Prognostic Significance of Epithelial-Mesenchymal and Mesenchymal-Epithelial Transition Protein Expression in Non–Small Cell Lung Cancer

Use of narrow‐range peptide IEF to improve detection of lung adenocarcinoma markers in plasma and pleural effusion

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