N-glycan structures and associated gene expression reflect the characteristic N-glycosylation pattern of human hematopoietic stem and progenitor cells

Hemmoranta, Heidi; Satomaa, Tero; Blomqvist, Maria; Heiskanen, Annamari; Aitio, Olli; Saarinen, Jyrki; Natunen, Jari; Partanen, Jukka; Laine, Jarmo; Jaatinen, Taina

doi:10.1016/j.exphem.2007.05.006

Cited by 54 publications

(72 citation statements)

References 81 publications

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“…Of these binders, antibodies against SSEA-3, SSEA-4, Tra-1-60, Tra-1-81 [25,26] have been generally accepted as tools for embryonic stem cell validation [27]. We have documented the glycan structures in hematopoietic stem cells and further verified glycosyltransferase gene expression profiles that are consistent with observed glycan structures [28].…”

Section: Introductionmentioning

confidence: 60%

“…Cells were prepared for glycan analysis essentially as described [28]. Cells were washed with PBS, scraped, collected with PBS and centrifuged for 5 min at 400×g.…”

Section: Glycan Isolationmentioning

confidence: 99%

“…For each N-glycan analysis, 300,000 to 500,000 cells were prepared. O-glycans were isolated from the de-N-glycosylated protein samples recovered during the precipitation-extraction purification step [28]. For subsequent O-glycan isolation, multiple glycoprotein samples were pooled.…”

Section: Glycan Isolationmentioning

confidence: 99%

“…Asparagine-linked glycans were detached from cellular glycoproteins by F. meningosepticum peptide:N-glycosidase F (PNGase F) digestion (Calbiochem, San Diego). The released asparaginelinked glycans (N-glycans) were purified for analysis by organic extraction-precipitation and miniaturized solid-phase extraction steps as described [28]. Serine-and threoninelinked glycans (O-glycans) were detached from cellular glycoproteins by non-reductive β-elimination with saturated ammonium carbonate in concentrated ammonia at 60°C [31].…”

Section: Glycan Isolationmentioning

confidence: 99%

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Glycomics of bone marrow-derived mesenchymal stem cells can be used to evaluate their cellular differentiation stage

et al. 2008

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Human mesenchymal stem cells (MSCs) are adult multipotent progenitor cells. They hold an enormous therapeutic potential, but at the moment there is little information on the properties of MSCs, including their surface structures. In the present study, we analyzed the mesenchymal stem cell glycome by using mass spectrometric profiling as well as a panel of glycan binding proteins. Structural verifications were obtained by nuclear magnetic resonance spectroscopy, mass spectrometric fragmentation, and glycosidase digestions. The MSC glycome was compared to the glycome of corresponding osteogenically differentiated cells. More than one hundred glycan signals were detected in mesenchymal stem cells and osteoblasts differentiated from them. The glycan profiles of MSCs and osteoblasts were consistently different in biological replicates, indicating that stem cells and osteoblasts have characteristic glycosylation features. Glycosylation features associated with MSCs rather than differentiated cells included high-mannose type N-glycans, linear poly-N-acetyllactosamine chains and α2-3-sialylation.

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Section: Introductionmentioning

confidence: 60%

“…Cells were prepared for glycan analysis essentially as described [28]. Cells were washed with PBS, scraped, collected with PBS and centrifuged for 5 min at 400×g.…”

Section: Glycan Isolationmentioning

confidence: 99%

Section: Glycan Isolationmentioning

confidence: 99%

Section: Glycan Isolationmentioning

confidence: 99%

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Glycomics of bone marrow-derived mesenchymal stem cells can be used to evaluate their cellular differentiation stage

et al. 2008

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“…In two reports by Wearne et al [68,69], cell-surface glycans present on BG01 hESC that had been differentiated for 12 days as embryoid bodies (EB) and the changes in the carbohydrates that occur between 12-28 days of differentiation as EB were analyzed using fluorescence microscopy with fluorescein-labeled lectins and antibodies specific for carbohydrate epitopes. In another study, MALDI-TOF MS profiling, NMR structural analysis, and lectin-based flow cytometry were used to compare the N-glycan structures of cord blood-derived CD133 + and CD133 -cells [70]. The results were correlated to gene expression analysis of glycosyltransferases and glycosidases.…”

Section: Glycoprotein Enrichment Methods -A Sweet Source For Stem Celmentioning

confidence: 99%

A novel role for proteomics in the discovery of cell‐surface markers on stem cells: Scratching the surface

Gundry

Boheler

Eyk

et al. 2008

Proteomics Clinical Apps

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The concept of cell-based therapy has been advocated as a novel approach for treating diseases or conditions where regeneration of cells, tissue and/or potentially organs is required. A promising source for cell-replacement therapies is provided by stem cells, but the success of this approach will ultimately rely on the ability to isolate primary stem or progenitor cells. Cell-surface protein markers will play a critical role in this step. Current methodologies for the identification of cell-surface protein markers rely primarily on antibody availability and flow cytometry, but many cell-surface proteins remain undetectable. Proteomic technologies now offer the possibility to specifically identify and investigate the cell-surface subproteome in a quantitative and discovery-driven manner. Once a cell surface protein marker panel has been identified by MS and the antibodies become available, the panel should permit the identification, tracking, and/or isolation of stem or progenitor cells that may be appropriate for therapeutics. This review provides a context for the use of proteomics in discovering new cell-surface markers for stem cells.

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The most reliable surface marker for the identification of colorectal cancer stem‐like cells: A systematic review and meta‐analysis

Abbasian

Mousavi

Arab‐Bafrani

et al. 2018

Journal Cellular Physiology

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Several surface markers have been proposed for the identification and characterization of colorectal cancer stem‐like cells (CR‐CSLCs). However, their reliability in CR‐CSLCs identification remains controversial. This study evaluated the correlation between all candidate surface marker's expression and CSLCs properties (tumorigenicity) through monitoring in vivo tumor incidence and final tumor volume. PubMed, Web of Science, and Scopus databases were systematically searched until November 2017. A total of 27 studies were found that met the inclusion criteria for cluster of differentiation 133 (CD133) and CD44 markers. Results indicated that either CD133 or CD44 positive cells caused about twofold increase in tumor volume compared with the negative cells (p < 0.05). In two groups of cells derived from primary tumors and cell lines, CD133 + cells had 25 and 1.45 times higher tumor incidence potential than CD133 − cells, respectively ( p < 0.05). Also, cohort evaluation showed that CD133 overexpression at protein level is a marker of poor overall survival in colorectal cancer (CRC) patients. While CD44 + cells displayed twofold tumorigenicity compared with the negative cells ( p < 0.05), combination of CD44 and CD133 showed about sevenfold tumorigenicity potential ( p < 0.05). In conclusion, the present meta‐analysis suggests that CD133 is a robust biomarker to identify primary tumor CSLCs and can be proposed as a prognostic marker of CRC patient whereas it should be used with caution in cell lines. It seems to be more reliable to use CD133 in combination with CD44 as target biomarkers for the isolation of CR‐CSLCs in both cell line and primary tumor cells populations.

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N-glycan structures and associated gene expression reflect the characteristic N-glycosylation pattern of human hematopoietic stem and progenitor cells

Cited by 54 publications

References 81 publications

Glycomics of bone marrow-derived mesenchymal stem cells can be used to evaluate their cellular differentiation stage

Glycomics of bone marrow-derived mesenchymal stem cells can be used to evaluate their cellular differentiation stage

A novel role for proteomics in the discovery of cell‐surface markers on stem cells: Scratching the surface

The most reliable surface marker for the identification of colorectal cancer stem‐like cells: A systematic review and meta‐analysis

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