N-desulfated and reacetylated modification of heparin modulates macrophage polarization

Zhu, Min; Wu, Xiao‐Tao; Sun, Jun; Zhou, Zhou; Kang, Mingzhu; Hu, Yiwei; Teng, Liping

doi:10.1016/j.ijbiomac.2022.12.213

Cited by 4 publications

(2 citation statements)

References 47 publications

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“…28 Nonetheless, the biological activity of HSS is known to be significantly impacted by subtle variations in its sulfation pattern. 29,30 For instance, N-and 2-O desulfation of HSS totally impairs its ability to bind to the fibroblast growth factor 2 (FGF-2), which, in turn, compromises FGF-induced signaling events. Conversely, desulfation at position 6-O does not affect FGF-2 binding but instead interferes with FGF-1 receptor activation, which blocks RAS-ERK signaling and, hence, suppresses angiogenesis.…”

Section: Introductionmentioning

confidence: 99%

“…HSS is known to control cell adhesion, spreading, migration, proliferation, and differentiation by interacting with particular cell surface receptors, like integrins and selectins, and by modulating the secretion of growth factors like fibroblast growth factors (FGFs), platelet-derived growth factors (PDGFs), VEGF, and transforming growth factor-beta (TGF-β) . Nonetheless, the biological activity of HSS is known to be significantly impacted by subtle variations in its sulfation pattern. , For instance, N- and 2-O desulfation of HSS totally impairs its ability to bind to the fibroblast growth factor 2 (FGF-2), which, in turn, compromises FGF-induced signaling events. Conversely, desulfation at position 6-O does not affect FGF-2 binding but instead interferes with FGF-1 receptor activation, which blocks RAS-ERK signaling and, hence, suppresses angiogenesis. , Similarly, a higher degree of sulfation raises HSS’s binding affinity for VEGF, increasing the growth factor’s absorption and sequestration.…”

Section: Introductionmentioning

confidence: 99%

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Heparinized Acellular Hydrogels for Magnetically Induced Wound Healing Applications

Pires,

Silva,

Ferreira

et al. 2024

ACS Appl. Mater. Interfaces

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The control of angiogenesis has the potential to be used for regulation of several pathological and physiological processes, which can be instrumental on the development of anticancer and wound healing therapeutical approaches. In this study, mesenchymal stem/stromal cells (MSCs) were seeded on magnetic-responsive gelatin, with or without heparin functionalization, and exposed to a static 0.08 T magnetic field (MF), for controlling their anti-inflammatory and angiogenic activity, with the aim of accelerating tissue healing. For the first time, it was examined how the amount of heparin and magnetic nanoparticles (MNPs) distributed on gelatin scaffolds affected the mechanical properties of the hydrogels and the morphology, proliferation, and secretome profiling of MSCs. The findings demonstrated that the addition of MNPs and heparin affects the hydrogel swelling capacity and renders distinct MSC proliferation rates. Additionally, MF acts as a topographical cue to guide MSCs alignment and increases the level of expression of specific genes and proteins that promote angiogenesis. The results also suggested that the presence of higher amounts of heparin (10 μg/cm 3 ) interferes with the secretion and limits the capacity of angiogenic factors to diffuse through the hydrogel and into the culture medium. Ultimately, this study shows that acellular heparinized hydrogels efficiently retain the angiogenic growth factors released by magnetically stimulated MSCs thus rendering superior wound contraction (55.8% ± 0.4%) and cell migration rate (49.4% ± 0.4%), in comparison to nonheparinized hydrogels (35.2% ± 0.7% and 37.8% ± 0.7%, respectively). Therefore, these heparinized magnetic hydrogels can be used to facilitate angiogenesis in various forms of tissue damage including bone defects, skin wounds, and cardiovascular diseases, leading to enhanced tissue regeneration.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Heparinized Acellular Hydrogels for Magnetically Induced Wound Healing Applications

Pires,

Silva,

Ferreira

et al. 2024

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

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TAM‐Hijacked Immunoreaction Rescued by Hypoxia‐Pathway‐Intervened Strategy for Enhanced Metastatic Cancer Immunotherapy

Jiang,

Wang,

Wang

et al. 2023

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Immunotherapy is regarded as a prospective strategy against metastatic cancer. However, tumor‐associated macrophages (TAMs), which accumulate in hypoxic tumor microenvironment, reduce the effectiveness of immunotherapy by blocking or “hijacking” the initiation of the immune response. Here, a novel tumor‐targeted nanoplatform loaded with hypoxia‐pathway‐intervened docosahexaenoic acid (DHA) and chemotherapeutic drug carfilzomib (CFZ) is developed, which realizes the rescue of TAM‐hijacked immune response and effective metastatic cancer immunotherapy. DHA is conjugated to fucoidan (Fuc) via a reduction cleavable selenylsulfide bond (SSe) for micelle preparation, and CFZ is encapsulated in the hydrophobic cores of micelles. The functionalized nanoplatforms (Fuc─SSe─DHA (FSSeD)–CFZs) induce immunogenic cell death, inhibit hypoxia‐inducible factor‐1α expression, and improve immunosuppression by TAM suppression. FSSeD–CFZs enhance immune response against primary tumor development and metastasis formation. In brief, the novel rescue strategy for TAM‐hijacked immunoreaction by inhibiting hypoxia pathway has the potential and clinically translational significance for enhanced metastatic cancer immunotherapy.

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Small-diameter PCL/PU vascular graft modified with heparin-aspirin compound for preventing the occurrence of acute thrombosis

Zhou

Xie

et al. 2023

International Journal of Biological Macromolecules

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N-desulfated and reacetylated modification of heparin modulates macrophage polarization

Cited by 4 publications

References 47 publications

Heparinized Acellular Hydrogels for Magnetically Induced Wound Healing Applications

Heparinized Acellular Hydrogels for Magnetically Induced Wound Healing Applications

TAM‐Hijacked Immunoreaction Rescued by Hypoxia‐Pathway‐Intervened Strategy for Enhanced Metastatic Cancer Immunotherapy

Small-diameter PCL/PU vascular graft modified with heparin-aspirin compound for preventing the occurrence of acute thrombosis

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