N-Cadherin Is Critical for the Survival of Germ Cells, the Formation of Steroidogenic Cells, and the Architecture of Developing Mouse Gonads

Piprek, Rafał P.; Kolasa, Michał; Podkowa, Dagmara; Kloc, Małgorzata; Kubiak, Jacek Z.

doi:10.3390/cells8121610

Cited by 13 publications

(9 citation statements)

References 53 publications

(75 reference statements)

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“…The mouse embryos with a mutation in E-cadherin or N-cadherin die at the 32-cell stage and E10 stage, respectively [64,65]. To overcome the lethality, we performed conditional knockouts of E-or N-cadherin in the germ cells or somatic cells of developing mouse gonads [62,66].…”

Section: Cadherins In Gonad Developmentmentioning

confidence: 99%

The Central Role of Cadherins in Gonad Development, Reproduction, and Fertility

Piprek

Kloc

Mizia

et al. 2020

IJMS

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Cadherins are a group of membrane proteins responsible for cell adhesion. They are crucial for cell sorting and recognition during the morphogenesis, but they also play many other roles such as assuring tissue integrity and resistance to stretching, mechanotransduction, cell signaling, regulation of cell proliferation, apoptosis, survival, carcinogenesis, etc. Within the cadherin superfamily, E- and N-cadherin have been especially well studied. They are involved in many aspects of sexual development and reproduction, such as germline development and gametogenesis, gonad development and functioning, and fertilization. E-cadherin is expressed in the primordial germ cells (PGCs) and also participates in PGC migration to the developing gonads where they become enclosed by the N-cadherin-expressing somatic cells. The differential expression of cadherins is also responsible for the establishment of the testis or ovary structure. In the adult testes, N-cadherin is responsible for the integrity of the seminiferous epithelium, regulation of sperm production, and the establishment of the blood–testis barrier. Sex hormones regulate the expression and turnover of N-cadherin influencing the course of spermatogenesis. In the adult ovaries, E- and N-cadherin assure the integrity of ovarian follicles and the formation of corpora lutea. Cadherins are expressed in the mature gametes and facilitate the capacitation of sperm in the female reproductive tract and gamete contact during fertilization. The germ cells and accompanying somatic cells express a series of different cadherins; however, their role in gonads and reproduction is still unknown. In this review, we show what is known and unknown about the role of cadherins in the germline and gonad development, and we suggest topics for future research.

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Section: Cadherins In Gonad Developmentmentioning

confidence: 99%

The Central Role of Cadherins in Gonad Development, Reproduction, and Fertility

Piprek

Kloc

Mizia

et al. 2020

IJMS

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“…We also performed a weighted gene co-expression network analysis on our sequencing results, looked for modules that are synergistically expressed, and explored the hub genes in the regulatory network. We found that many hub genes such [25,29].…”

Section: Discussionmentioning

confidence: 77%

“…N-cadherin coded by the Cdh2 gene is important for maintenance of the testis cord structure and is required for the formation of steroidogenic cells. N-cadherin plays a major role in gonad differentiation, structuralization, and function [25]. Disruption of the spermatogenic cell-specific mouse enolase 4…”

Section: Co-expression Network Analysis Identified Several Hub Genes mentioning

confidence: 99%

Histone H3 methylation orchestrates transcriptional program in mouse spermatogenic cell line

et al. 2020

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Changes in histone modifications always correlate with altered transcriptional activities of genes. Recent studies have shown that the mutation of certain lysine residues to methionine in the histone variant H3.3 can act as a valuable tool to reduce specific H3 methylation levels. In our study, we used the mouse spermatogenic cell line GC-2 as a model to generate cells stably expressing H3.3 K4, H3.3 K9, H3.3 K27, and H3.3 K36M. The expression of these H3.3 K-to-M mutants influenced the expression of different subsets of genes, and a total of 891 differentially expressed genes were identified through global gene expression profiling. Moreover, the H3.3 K-to-M transgenes, especially H3.3 K36M, impacted the expression of endogenous retrovirus ERVK. This study gives a global view of how different H3 modifications regulate transcriptomes in spermatogenic cell lines, and identifies potential targets of H3 modifications in male germ line.

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“…The knockout of N-cadherin in the germ cells led to apoptosis, and reduction of germ cell number [55]. The knockout of N-cadherin in gonadal somatic cells, in contrast to the E-cadherin knockout, disrupted the gonad structure.…”

Section: Cadherins In Gonad Developmentmentioning

confidence: 96%

“…The mouse embryos with a mutation in E-cadherin or N-cadherin die at the 32-cell stage, and E10 stage, respectively [53,54]. To overcome the lethality, we performed conditional knockouts of E-or N-cadherin in the germ cells or somatic cells of developing mouse gonads [51,55].…”

Section: Cadherins In Gonad Developmentmentioning

confidence: 99%

The Central Role of Cadherins in Gonad Development, Reproduction and Fertility

Piprek

Kloc

Mizia

et al. 2020

Preprint

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Cadherins are a group of membrane proteins responsible for cell adhesion. They are crucial for cell sorting and recognition during the morphogenesis, but also play many other roles such as assuring tissue integrity and resistance to stretching, mechanotransduction, cell signaling, regulation of cell proliferation, apoptosis, survival, carcinogenesis, etc. Within the cadherin superfamily, the E- and N-cadherin have been especially well studied. They are involved in many aspects of sexual development and reproduction, such as germline development and gametogenesis, gonad development and functioning, and fertilization. E-cadherin is expressed in the primordial germ cells, (PGCs) and also participates in PGC migration to the developing gonads where they become enclosed by the N-cadherin-expressing somatic cells. The differential expression of cadherins is also responsible for the establishment of the testis or ovary structure. In the adult testes, the N-cadherin is responsible for the integrity of the seminiferous epithelium, regulation of sperm production, and the establishment of the blood-testis barrier. Sex hormones regulate the expression and turnover of N-cadherin influencing the course of spermatogenesis. In the adult ovaries, E- and N-cadherin assure the integrity of ovarian follicles and the formation of corpora lutea. Cadherins are expressed in the mature gametes, and facilitate the capacitation of sperm in the female reproductive tract, and gamete contact during fertilization. The germ cells and accompanying somatic cells express a series of different cadherins, however, their role in gonads and reproduction is still unknown. In this review, we show what is known and unknown about the role of cadherins in the germline and gonad development, and suggest the topics for future research.

show abstract

N-Cadherin Is Critical for the Survival of Germ Cells, the Formation of Steroidogenic Cells, and the Architecture of Developing Mouse Gonads

Cited by 13 publications

References 53 publications

The Central Role of Cadherins in Gonad Development, Reproduction, and Fertility

The Central Role of Cadherins in Gonad Development, Reproduction, and Fertility

Histone H3 methylation orchestrates transcriptional program in mouse spermatogenic cell line

The Central Role of Cadherins in Gonad Development, Reproduction and Fertility

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