N‐cadherin and M‐cadherin are sequentially expressed in myoblast populations contributing to the first and second waves of myogenesis in the trout (<i>Oncorhynchus mykiss</i>)

Rescan, Pierre‐Yves; Rallière, Cécile; Lebret, Véronique

doi:10.1002/jez.b.21443

Cited by 2 publications

(2 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among the large set of membrane proteins up-regulated in hyperplastic zones were found many genes encoding Ig-domain containing transmembrane proteins such as Kin of irre 3, Ncam, N-cadherin, M-cadherin and Brother of CDO, all of which are known to influence cell-cell interactions during myoblast differentiation or fusion [30]. Surprisingly, in contrast to M-cadherin, which is expressed in differentiating myoblasts located at the periphery of the expanding myotome [31], Kin of Irre 3 and brother of CDO were found to be transcribed primarily in the external dermomyotome-like epithelium, similar to what is observed for N-cadherin [31]. This indicates that the transcription of Kin of Irre 3 and brother of CDO takes place early in the myogenic progenitor cells that participate in the second wave of myofibre formation and does not depend on MRF activity.…”

Section: Discussionmentioning

confidence: 99%

Gene expression profiling of the hyperplastic growth zones of the late trout embryo myotome using laser capture microdissection and microarray analysis

et al. 2013

Self Cite

View full text Add to dashboard Cite

BackgroundA unique feature of fish is that new muscle fibres continue to be produced throughout much of the life cycle; a process termed muscle hyperplasia. In trout, this process begins in the late embryo stage and occurs in both a discrete, continuous layer at the surface of the primary myotome (stratified hyperplasia) and between existing muscle fibres throughout the myotome (mosaic hyperplasia). In post-larval stages, muscle hyperplasia is only of the mosaic type and persists until 40% of the maximum body length is reached. To characterise the genetic basis of myotube neoformation in trout, we combined laser capture microdissection and microarray analysis to compare the transcriptome of hyperplastic regions of the late embryo myotome with that of adult myotomal muscle, which displays only limited hyperplasia.ResultsGene expression was analysed using Agilent trout oligo microarrays. Our analysis identified more than 6800 transcripts that were significantly up-regulated in the superficial hyperplastic zones of the late embryonic myotome compared to adult myotomal muscle. In addition to Pax3, Pax7 and the fundamental myogenic basic helix-loop-helix regulators, we identified a large set of up-regulated transcriptional factors, including Myc paralogs, members of Hes family and many homeobox-containing transcriptional regulators. Other cell-autonomous regulators overexpressed in hyperplastic zones included a large set of cell surface proteins belonging to the Ig superfamily. Among the secreted molecules found to be overexpressed in hyperplastic areas, we noted growth factors as well as signalling molecules. A novel finding in our study is that many genes that regulate planar cell polarity (PCP) were overexpressed in superficial hyperplastic zones, suggesting that the PCP pathway is involved in the oriented elongation of the neofibres.ConclusionThe results obtained in this study provide a valuable resource for further analysis of novel genes potentially involved in hyperplastic muscle growth in fish. Ultimately, this study could yield insights into particular genes, pathways or cellular processes that may stimulate muscle regeneration in other vertebrates.

show abstract

Section: Discussionmentioning

confidence: 99%

Gene expression profiling of the hyperplastic growth zones of the late trout embryo myotome using laser capture microdissection and microarray analysis

et al. 2013

Self Cite

View full text Add to dashboard Cite

show abstract

“…Later in development, CILP1 expression disappeared medio-laterally in differentiating fast embryonic myoblasts (Fig. 4B), following a pattern similar to that of N-cadherin (Rescan et al, 2012) and Seraf, an EGF-like repeat autocrine factor (Dumont et al, 2008). As the somites matured rostro-caudally, CILP1 expression became restricted to the dermomyotome-like …”

mentioning

confidence: 82%

CILP1 is dynamically expressed in the developing musculoskeletal system of the trout

Rallière¹,

Frétaud²,

Thermes³

et al. 2015

Int. J. Dev. Biol.

Self Cite

View full text Add to dashboard Cite

An in situ screen for genes expressed in the skeletal muscle of eyed-stage trout embryos led to the identification of a transcript encoding a polypeptide related to CILP1, a secreted glycoprotein present in the extracellular matrix. In situ hybridisation in developing trout embryos revealed that CILP1 expression was initially detected in fast muscle progenitors of the early somite. Later, CILP1 expression was down-regulated medio-laterally in differentiating fast muscle cells, to become finally restricted to the undifferentiated muscle progenitors forming the dermomyotomelike epithelium at the surface of the embryonic myotome. At the completion of somitogenesis, CILP1 expression was concentrated in the myoseptal/tendon cells that develop between adjacent myotomes but was excluded from the skeletogenic cells of the vertebral axis to which the most medial myoseptal/tendon cells attach. Overall, our work shows that muscle cells and myoseptal/ tendon cells contribute dynamically and cooperatively to the production of CILP1 during ontogeny of the trout musculoskeletal system. KEY WORDS: CILP1, somite, myotome, dermomyotome, myoseptal cell, teleostIn fish, the musculoskeletal system is a multicomponent system composed of W-shaped myomeres, myosepta and the axial skeleton. Myomeres arise from somites that are generated repeatedly from the presomitic mesoderm in an anterior to posterior progression. Two main muscle fibre types differentiate within somites: the superficial slow muscle fibres and the deep fast muscle fibres. Embryonic slow muscle fibres originate from adaxial cells, initially deep in the somite, that migrate radially to reach the lateral surface of the developing myotome, while embryonic fast muscle fibres derive from myogenic muscle cell precursors located in the posterior somitic compartment (for review see Bryson-Richardson and Currie, 2008). Cells of the anterior somitic compartment form a superficial dermomyotome-like epithelium surrounding the slow muscle fibres. This epithelium provides the myogenic precursor cells necessary for the growth of the embryonic myotome (Hollway et al., 2007;Stellabotte et al., 2007, Steinbacher et al., 2008. The fish myosepta that separate adjacent myomeres are medially inserted on the bony axial skeleton and are laterally connected to the collagenous dermis. Like tendons in amniotes, fish myosepta serve as transmitters of muscle contractility to bones. In the developing fish embryo, the myosepta are initially acellular and composed of matricial compounds such as fibronectin, laminin and collagens (Henry et al., 2005;Charvet et al., 2011;Bricard et al., 2014 ent in the intersomitic space (Charvet et al., 2011;Bricard et al., 2014;Chen and Galloway., 2014;Subramanian and Schilling., 2014). These myoseptal cells are considered homologous to axial tenocytes in amniotes: they express scleraxis, tenomodulin and tendon associated collagens (Bricard et al., 2014;Chen and Galloway, 2014) and, like axial tenocytes in amniotes (Brent et al., 2003), they probably originat...

show abstract

N‐cadherin and M‐cadherin are sequentially expressed in myoblast populations contributing to the first and second waves of myogenesis in the trout (Oncorhynchus mykiss)

Cited by 2 publications

References 41 publications

Gene expression profiling of the hyperplastic growth zones of the late trout embryo myotome using laser capture microdissection and microarray analysis

Gene expression profiling of the hyperplastic growth zones of the late trout embryo myotome using laser capture microdissection and microarray analysis

CILP1 is dynamically expressed in the developing musculoskeletal system of the trout

Contact Info

Product

Resources

About