n-Butylamine for Improving the Efficiency of Untargeted Mass Spectrometry Analysis of Plasma Metabolite Composition

Abstract

A comparative study of the impact of n-butylamine and traditionally used additives (ammonium hydroxide and formic acid) on the efficiency of the electrospray ionization (ESI) process for the enhancement of metabolite coverage was performed by direct injection mass spectrometry (MS) analysis in negative mode. Evaluation of obtained MS data showed that n-butylamine is one of the most effective additives for the analysis of metabolite composition in ESI in negative ion mode (ESI(−)) The limitations of the use of n-butylamine and other alkylamines in the analysis of metabolic composition and a decontamination procedure that can reduce MS device contamination after their application are discussed. The proposed procedure allows the performance of high-sensitivity analysis of low-molecular-weight compounds on the same MS device in both polarities.

Metabolomics Community in Russia: History of Development, Key Participants, and Results

“…The possibilities of such analysis are described in detail in the review by Lokhov et al [ 41 ]. The greatest success in this field was achieved using direct injection of a low-molecular fraction of blood sample into an electrospray ionization source of a quadrupole time-of-flight mass spectrometer (DIMS, direct infusion mass spectrometry) [ 42 , 43 ]. The resulting mass spectrum reflected the picture of the metabolome that with subsequent statistical analysis in the group with healthy volunteers metabolomes made it possible to establish the normativity of the metabotype, identify xenobiotics, detect low molecular weight biomarkers of diseases, and apply metabolomic diagnostic signatures [ 41 ].…”

Breaking the Equilibrium and Improving the Yield of Schiff Base Reactions by Pervaporation: Application to a Reaction Involving n-butylamine and Benzaldehyde

“…Moreover, methods using solvents, such as tetramethyl orthosilicate or trimethyl orthoformate, which remove water in the reaction medium, have also been separately reported by Love et al [16] and Look et al [15]. n-Butylamine is used in many procedures both in industry and in medicine [38,39].…”

Rendering A Chiral Screening Step In Supercritical Fluid Chromatography Mass-Spectrometry Compatible

“…These additives suppress the ionization of the analytes due to their relatively strong ionpairing properties. In addition, the alkyl amines are known to contaminate the MS source, which is not favorable either [23][24][25].…”