2003
DOI: 10.1093/glycob/cwh010
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N- and O-glycans of recombinant human C1 inhibitor expressed in the milk of transgenic rabbits

Abstract: Human C1 inhibitor (hC1INH) is a therapeutic N, O-glycoprotein with a growing number of clinical applications, but the current natural supplies are not likely to meet the clinical demands. Therefore, recombinant approaches are of interest, whereby specific attention has to be paid to the generated glycosylation patterns. Here, the N,O-glycoprotein was expressed in the mammary gland of transgenic rabbits and subjected to glycan analysis. After release of the N-glycans of recombinant-rabbit human C1 inhibitor (r… Show more

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Cited by 91 publications
(72 citation statements)
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References 48 publications
(53 reference statements)
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“…To date several proteins have been expressed in the milk of transgenic animals in quantities ranging from a few micrograms to several grams per liter. Some examples are: human lactoferrin in mice [2] and cows [3]; a1-antitrypsin [4] and C1 inhibitor [5] in rabbits; human anti-thrombin [6] and human tissue-type plasminogen activator [7,8] in goats; human Factor VIII [9] and human Protein C [10] in pigs; human a-glucosidase in mice [11] and rabbits [12] and human erythropoietin in rabbits [13] and pigs [14]. However, the functionality and specificity of mammary gland glycosylation is, as yet, incompletely understood in comparison with other mammalian cell lines.…”
mentioning
confidence: 99%
“…To date several proteins have been expressed in the milk of transgenic animals in quantities ranging from a few micrograms to several grams per liter. Some examples are: human lactoferrin in mice [2] and cows [3]; a1-antitrypsin [4] and C1 inhibitor [5] in rabbits; human anti-thrombin [6] and human tissue-type plasminogen activator [7,8] in goats; human Factor VIII [9] and human Protein C [10] in pigs; human a-glucosidase in mice [11] and rabbits [12] and human erythropoietin in rabbits [13] and pigs [14]. However, the functionality and specificity of mammary gland glycosylation is, as yet, incompletely understood in comparison with other mammalian cell lines.…”
mentioning
confidence: 99%
“…The proteins lacking sialic acid generally have an excess of terminal mannose which favours their rapid elimination from the blood. Interestingly, the protein C inhibitor [33] and a coagulation blood factor (unpublished data) secreted in rabbit milk did not contain fucose. This suggests that fucosylation is poorly active in the rabbit mammary gland.…”
Section: Production Of Pharmaceutical Proteins In Milkmentioning
confidence: 98%
“…Rabbit and pig milking can be achieved with no particular difficulty using adapted machines. Rabbits and pigs appeared more able than ruminants to perform some posttranslational modifications as judged by the examination of a few proteins such as human protein C [36] and human protein C inhibitor [33]. This is particularly the case for glycosylation.…”
Section: Production Of Pharmaceutical Proteins In Milkmentioning
confidence: 99%
See 1 more Smart Citation
“…A new approach for the development of a human C1 inhibitor for treatment of HAE attacks was developed by a second Dutch company, Pharming[20]. This company pioneered the concept of isolating the human gene for a plasma protein and fusing it to a bovine casein promoter.…”
Section: Recombinant C1 Inhibitormentioning
confidence: 99%