N- and C-terminal non-conserved residues contribute to transactivation by a sea anemone (<i>Nematostella vectensis</i>) NF-κB transcription factor

Alshanbayeva, Anar; Thomas, Ajith J.; Tremblay, Monique; Abbas, Majed; Abdurrob, Fatema; Almojel, Talal; Aparicio, A. J. R.; Asarpota, Dhishant; Ayers, Ashley; Aziz, Amar; Bishop, Jacob; Christie, T.; Jie, Melissa Chua Ming; Chung, Oliver; Dhar, Nikhil Ranjan; Diedrich, Alexa; Fortin, Chelsea L.; He, Qianjing; Heerboth, Sarah; Hok, Rosanna; Khedkar, Aditya; Kitchloo, Shweta; Lawlor, Caitlin; Leonard, Brian C.; Linderman, Shannon E.; Maloyan, Mariam; Miller, Lauren N.; Pak, Christine J.; Pandita, Abishy; Park, Isabel; Patel, Neil D.; Ramachandran, Janani; Reynoso, Marinaliz; Samaha, Yasmina; Thole, Grace; Turnbill, Jason; Xia, Linge; Zhu, Jing; Navarro, Caryn; Gilmore, Thomas D.

doi:10.1893/0005-3155-86.4.165

Cited by 3 publications

(1 citation statement)

References 8 publications

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“…2E). As we have shown previously (18,19,27), a GAL4-Nv-NF-κB fusion protein strongly activated transcription in yeast (approximately 600-fold) as compared to the GAL4 alone control. Therefore, the ability of the Aq-RHD sequences to activate transcription appears to be an intrinsic property of sequences within the N-terminal half of the protein, and this transactivaton activity is not apparent in the fulllength Aq-NF-κB protein.…”

Section: Aq-nf-κb Protein Contains Sequences That Bind Dna and Activa...supporting

confidence: 66%

Transcription factor NF-κB in a basal metazoan, the sponge, has conserved and unique sequences, activities, and regulation

Williams

Inge

Mansfield

et al. 2020

Developmental & Comparative Immunology

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Section: Aq-nf-κb Protein Contains Sequences That Bind Dna and Activa...supporting

confidence: 66%

Transcription factor NF-κB in a basal metazoan, the sponge, has conserved and unique sequences, activities, and regulation

Williams

Inge

Mansfield

et al. 2020

Developmental & Comparative Immunology

Self Cite

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A conserved Toll-like receptor-to-NF-κB signaling pathway in the endangered coral Orbicella faveolata

Williams

Fuess

Brennan

et al. 2018

Developmental & Comparative Immunology

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Transcription factor NF-κB in a basal metazoan, the sponge, has conserved and unique sequences, activities, and regulation

Williams¹,

Inge

Mansfield³

et al. 2019

Preprint

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Biological and biochemical functions of immunity transcription factor NF-κB in basal metazoans are largely unknown. Herein, we characterize transcription factor NF-κB from the demosponge Amphimedon queenslandica (Aq), in the phylum Porifera. Structurally and phylogenetically, the Aq-NF-κB protein is most similar to NF-κB p100 and p105 among vertebrate proteins, with an Nterminal DNA-binding/dimerization domain, a C-terminal Ankyrin (ANK) repeat domain, and a DNA binding-site profile more similar to human NF-κB proteins than Rel proteins. Aq-NF-κB also resembles the mammalian NF-κB protein p100 in that C-terminal truncation results in translocation of Aq-NF-κB to the nucleus and increases its transcriptional activation activity. Overexpression of a human or sea anemone IκB kinase (IKK) can induce C-terminal processing of Aq-NF-κB in vivo, and this processing requires C-terminal serine residues in Aq-NF-κB. Unlike human NF-κB p100, however, the C-terminal sequences of Aq-NF-κB do not effectively inhibit its DNAbinding activity when expressed in human cells. Tissue of another demosponge, a black encrusting sponge, contains NF-κB site DNAbinding activity and an NF-κB protein that appears mostly processed and in the nucleus of cells. NF-κB DNA-binding activity and processing is increased by treatment of sponge tissue with LPS. By transcriptomic analysis of A. queenslandica we identified likely homologs to many upstream NF-κB pathway components. These results present a functional characterization of the most ancient metazoan NF-κB protein to date, and show that many characteristics of mammalian NF-κB are conserved in sponge NF-κB, but the mechanism by which NF-κB functions and is regulated in the sponge may be somewhat different.

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N- and C-terminal non-conserved residues contribute to transactivation by a sea anemone (Nematostella vectensis) NF-κB transcription factor

Cited by 3 publications

References 8 publications

Transcription factor NF-κB in a basal metazoan, the sponge, has conserved and unique sequences, activities, and regulation

Transcription factor NF-κB in a basal metazoan, the sponge, has conserved and unique sequences, activities, and regulation

A conserved Toll-like receptor-to-NF-κB signaling pathway in the endangered coral Orbicella faveolata

Transcription factor NF-κB in a basal metazoan, the sponge, has conserved and unique sequences, activities, and regulation

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