n-Alkane biosynthetic hydrogen isotope fractionation is not constant throughout the growing season in the riparian tree Salix viminalis

Newberry, Sarah L.; Kahmen, Ansgar; Dennis, Paul F.; Grant, Alastair

doi:10.1016/j.gca.2015.05.001

Cited by 69 publications

(39 citation statements)

References 57 publications

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“…Most importantly, however, understanding the metabolic effects that shape the δ 2 H values of plant organic compounds will open new opportunities to utilize plant δ 2 H values to address the C metabolism of plants and ecosystems. While we show here that photosynthetic carbohydrate supply has a key effect on the δ 2 H values of plant organic compounds, previous studies have already employed δ 2 H values of n ‐alkanes or cellulose to indicate the C autonomy of plant tissues, plant organs or entire plants (Gamarra & Kahmen, ; Kimak et al ., ; Newberry et al ., ; Gebauer et al ., ). With our conceptual biochemical model, we can now explain why organic compounds in non‐C autonomous tissue with low photosynthetic carbohydrate supplies become 2 H‐enriched.…”

Section: Resultsmentioning

confidence: 98%

²H‐fractionations during the biosynthesis of carbohydrates and lipids imprint a metabolic signal on the δ²H values of plant organic compounds

et al. 2018

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Hydrogen (H) isotope ratio (δ H) analyses of plant organic compounds have been applied to assess ecohydrological processes in the environment despite a large part of the δ H variability observed in plant compounds not being fully elucidated. We present a conceptual biochemical model based on empirical H isotope data that we generated in two complementary experiments that clarifies a large part of the unexplained variability in the δ H values of plant organic compounds. The experiments demonstrate that information recorded in the δ H values of plant organic compounds goes beyond hydrological signals and can also contain important information on the carbon and energy metabolism of plants. Our model explains where H-fractionations occur in the biosynthesis of plant organic compounds and how these H-fractionations are tightly coupled to a plant's carbon and energy metabolism. Our model also provides a mechanistic basis to introduce H isotopes in plant organic compounds as a new metabolic proxy for the carbon and energy metabolism of plants and ecosystems. Such a new metabolic proxy has the potential to be applied in a broad range of disciplines, including plant and ecosystem physiology, biogeochemistry and palaeoecology.

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Section: Resultsmentioning

confidence: 98%

²H‐fractionations during the biosynthesis of carbohydrates and lipids imprint a metabolic signal on the δ²H values of plant organic compounds

et al. 2018

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“…This δD offset is known as biosynthetic fractionation (ε bio ). Some evidence suggests that ε bio is constant within a species (Sessions et al, 1999;Tipple et al, 2015) while other studies have found that ε bio may vary throughout the growing season (Newberry et al, 2015;Sachse et al, 2015). Among species, ε bio can vary by up to 65‰ (Kahmen et al, 2013b).…”

Section: Introductionmentioning

confidence: 99%

Hydrogen isotopes of n-alkanes and n-alkanoic acids as tracers of precipitation in a temperate forest and implications for paleorecords

Freimuth

Diefendorf

Lowell

2017

Geochimica et Cosmochimica Acta

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The hydrogen isotopic composition of leaf waxes (δD wax) primarily reflects that of plant source water. Therefore, sedimentary δD wax records are increasingly used to reconstruct the δD of past precipitation (δD p) and to investigate paleohydrologic changes. Such reconstructions rely on estimates of apparent fractionation (ε app) between δD p and the resulting δD wax. However, ε app values are modified by numerous environmental and biological factors during leaf wax production. As a result, ε app can vary widely among plant species and growth forms. This complicates estimation of accurate ε app values and presents a central challenge to leaf wax paleohydrology. During the 2014 growing season, we examined ε app in the five deciduous angiosperm tree species (Prunus serotina, Acer saccharinum, Quercus rubra, Quercus alba, and Ulmus americana) that dominate the temperate forest at Brown's Lake Bog, Ohio, USA. We sampled individuals of each species at weekly to monthly intervals from March to October and report δD values of n-C 29 alkanes (δD n-C29 alkane) and n-C 28 alkanoic acids (δD n-C28 acid), as well as xylem (δD xw) and leaf water (δD lw). n-Alkane synthesis was most intense 2-3 weeks after leaf emergence and ceased thereafter, whereas n-alkanoic acid synthesis continued throughout the entire growing season. During bud swell and leaf emergence, δD lw was a primary control on δD n-C29 alkane and δD n-C28 acid values, which stabilized once leaves became fully expanded. Metabolic shifts between young and mature leaves may be an important secondary driver of δD wax changes during leaf development. In mature autumn leaves of all species, the mean ε app for n-C 29 alkane (-107‰) was offset by approximately-19‰ from the mean ε app for n-C 28 alkanoic acid (-88‰). These results indicate that in temperate settings n-alkanes and n-alkanoic acids from deciduous trees are distinct with respect to their abundance, timing of synthesis, and ε app values.

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“…NADP + can be reduced to NADPH by the oxidation of sugars in the pentose‐phosphate cycle (OPPP), where the hydrogen derives from C‐bound H contained within sugars (Schmidt et al, ; Sessions, ; Sessions et al, ). These diverse hydrogen sources give rise to differences in the isotopic composition of NADPH among plant compartments (Schmidt et al, ) and have been proposed as mechanisms to account for interspecies variation in leaf wax n‐ alkane δ 2 H values (Newberry et al, ).…”

Section: Introductionmentioning

confidence: 99%

Variation in Hydrogen Isotope Composition Among Salt Marsh Plant Organic Compounds Highlights Biochemical Mechanisms Controlling Biosynthetic Fractionation

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n-Alkane biosynthetic hydrogen isotope fractionation is not constant throughout the growing season in the riparian tree Salix viminalis

Cited by 69 publications

References 57 publications

²H‐fractionations during the biosynthesis of carbohydrates and lipids imprint a metabolic signal on the δ²H values of plant organic compounds

²H‐fractionations during the biosynthesis of carbohydrates and lipids imprint a metabolic signal on the δ²H values of plant organic compounds

Hydrogen isotopes of n-alkanes and n-alkanoic acids as tracers of precipitation in a temperate forest and implications for paleorecords

Variation in Hydrogen Isotope Composition Among Salt Marsh Plant Organic Compounds Highlights Biochemical Mechanisms Controlling Biosynthetic Fractionation

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n-Alkane biosynthetic hydrogen isotope fractionation is not constant throughout the growing season in the riparian tree Salix viminalis

Cited by 69 publications

References 57 publications

2H‐fractionations during the biosynthesis of carbohydrates and lipids imprint a metabolic signal on the δ2H values of plant organic compounds

2H‐fractionations during the biosynthesis of carbohydrates and lipids imprint a metabolic signal on the δ2H values of plant organic compounds

Hydrogen isotopes of n-alkanes and n-alkanoic acids as tracers of precipitation in a temperate forest and implications for paleorecords

Variation in Hydrogen Isotope Composition Among Salt Marsh Plant Organic Compounds Highlights Biochemical Mechanisms Controlling Biosynthetic Fractionation

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²H‐fractionations during the biosynthesis of carbohydrates and lipids imprint a metabolic signal on the δ²H values of plant organic compounds

²H‐fractionations during the biosynthesis of carbohydrates and lipids imprint a metabolic signal on the δ²H values of plant organic compounds