N-acylphosphatidylethanolamine in the fish brain

Vas’kovskii, V. E.; Vysotskii, Mikhail V.

doi:10.1007/bf00574199

Cited by 2 publications

(3 citation statements)

References 18 publications

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“…In our search for NAPE in bony and cartilaginous fish we found that it is difficult to distinguish SLBPA and NAPE even by high-performance TLC, and chemical transformations are required to attribute an unknown lipid to one of these without employing physico-chemical methods of structure elucidation [30]. Later we demonstrated that reliable separation of SLBPA and NAPE requires the use of homemade HPTLC-plates coated with silica sol-bound microfractionated silica gel KSK, and low humidity [36].…”

Section: Discussionmentioning

confidence: 99%

“…Solvent systems for TLC were taken from Vaskovsky and Vysotskii [30]. To develop plates in the 1st direction the system used was chloroform-methanol-benzene-25% aqueous ammonia (60:15:10:1, by v/v/v/v).…”

Section: Methodsmentioning

confidence: 99%

“…The use of the solvent systems developed for one-dimensional TLC separation of low polarity phospholipids [30] resulted in good separation for some lipids (Fig. 4).…”

Section: Thin-layer Chromatographymentioning

confidence: 99%

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TLC and ³¹P‐NMR Analysis of Low Polarity Phospholipids

Vyssotski

MacKenzie

Scott

2008

Lipids

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High-performance TLC and (31)P-NMR were assessed as methods of observing the presence of numerous low polarity phospholipids: bis-phosphatidic acid (BPA), semi-lyso bis-phosphatidic acid (SLBPA), N-acyl phosphatidylethanolamine (NAPE), N-(1,1-dimethyl-3-oxo-butyl)-phosphatidylethanolamine (diacetone adduct of PE, DOBPE), N-acetyl PE, phosphatidylmethanol (PM), phosphatidylethanol (PEt), phosphatidyl-n-propanol (PP), phosphatidyl-n-butanol (PB). Both techniques are non-discriminative and do not require the prior isolation of individual lipids. It appears that 2D TLC is superior to (31)P NMR in the analysis of low polarity phospholipids. All phosphatidylalcohols were well separated by 2D TLC. However, some compounds which can present difficulty in separation by 2D-TLC (e.g., SLBPA and NAPE; or DOBPE and N-acetyl PE) were easily distinguished using (31)P NMR so the methods are complimentary. A disadvantage of 2D TLC is that Rf values can vary with different brands and batches of TLC plates. The chemical shifts of (31)P NMR were less variable, and so a library of standards may not be necessary for peak identification. Another advantage of (31)P NMR is the ease of quantification of phospholipids. The applicability of the methods was tested on natural extracts of fish brain and cabbage stem.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%