N-Acyl Homoserine Lactone-Mediated Quorum Sensing in Aeromonas veronii biovar sobria Strain 159: Identification of LuxRI Homologs

Chan, Xin Yue; How, Kah Yan; Yin, Wai Fong; Chan, Kok Gan

doi:10.3389/fcimb.2016.00007

Cited by 10 publications

(6 citation statements)

References 26 publications

(33 reference statements)

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“…The "signal" molecule in Gram-negative bacteria is N-acyl homoserine-lactone (AHL) and in Aeromonas, the AHL is able to modulate the host immune response [19,186,259]. Several studies have shown the importance of AHLs in regulating a range of biological functions such as biofilm formation, antibiotic production, and warming motility and the different QS systems harbor differences in their influences [260][261][262][263][264][265][266]. Chan et al [260] found in the whole genome of the strain A. veronii 159 sequences of QS-related genes, providing a model for exploring it roles in virulence and as a potential target for Aeromonas treatment.…”

Section: Quorum Sensingmentioning

confidence: 99%

“…Several studies have shown the importance of AHLs in regulating a range of biological functions such as biofilm formation, antibiotic production, and warming motility and the different QS systems harbor differences in their influences [260][261][262][263][264][265][266]. Chan et al [260] found in the whole genome of the strain A. veronii 159 sequences of QS-related genes, providing a model for exploring it roles in virulence and as a potential target for Aeromonas treatment. Talagrand-Reboul et al [266] focused their review on the knowledge of the biofilm and QS in Aeromonas, explaining that biofilm provides a high cell-density that allows interaction between bacteria with the QS systems in Aeromonas.…”

Section: Quorum Sensingmentioning

confidence: 99%

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An Update on the Genus Aeromonas: Taxonomy, Epidemiology, and Pathogenicity

2020

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The genus Aeromonas belongs to the Aeromonadaceae family and comprises a group of Gram-negative bacteria widely distributed in aquatic environments, with some species able to cause disease in humans, fish, and other aquatic animals. However, bacteria of this genus are isolated from many other habitats, environments, and food products. The taxonomy of this genus is complex when phenotypic identification methods are used because such methods might not correctly identify all the species. On the other hand, molecular methods have proven very reliable, such as using the sequences of concatenated housekeeping genes like gyrB and rpoD or comparing the genomes with the type strains using a genomic index, such as the average nucleotide identity (ANI) or in silico DNA–DNA hybridization (isDDH). So far, 36 species have been described in the genus Aeromonas of which at least 19 are considered emerging pathogens to humans, causing a broad spectrum of infections. Having said that, when classifying 1852 strains that have been reported in various recent clinical cases, 95.4% were identified as only four species: Aeromonas caviae (37.26%), Aeromonas dhakensis (23.49%), Aeromonas veronii (21.54%), and Aeromonas hydrophila (13.07%). Since aeromonads were first associated with human disease, gastroenteritis, bacteremia, and wound infections have dominated. The literature shows that the pathogenic potential of Aeromonas is considered multifactorial and the presence of several virulence factors allows these bacteria to adhere, invade, and destroy the host cells, overcoming the immune host response. Based on current information about the ecology, epidemiology, and pathogenicity of the genus Aeromonas, we should assume that the infections these bacteria produce will remain a great health problem in the future. The ubiquitous distribution of these bacteria and the increasing elderly population, to whom these bacteria are an opportunistic pathogen, will facilitate this problem. In addition, using data from outbreak studies, it has been recognized that in cases of diarrhea, the infective dose of Aeromonas is relatively low. These poorly known bacteria should therefore be considered similarly as enteropathogens like Salmonella and Campylobacter.

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Section: Quorum Sensingmentioning

confidence: 99%

Section: Quorum Sensingmentioning

confidence: 99%

An Update on the Genus Aeromonas: Taxonomy, Epidemiology, and Pathogenicity

2020

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“…Near to the strains synthesizing AI, luminescence of biosensor cells visible to the naked eye were observed. A sequence analysis of 16S rRNA genes showed that isolated AI-synthesizing strains belong to the Aeromonas veronii or Aeromonas hydrophila species, for which the presence of type I QS system is known [ 30 ].…”

Section: Resultsmentioning

confidence: 99%

Influence of the luxR Regulatory Gene Dosage and Expression Level on the Sensitivity of the Whole-Cell Biosensor to Acyl-Homoserine Lactone

et al. 2021

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Aliivibrio fischeri LuxR and Aliivibrio logei LuxR1 and LuxR2 regulatory proteins are quorum sensing transcriptional (QS) activators, inducing promoters of luxICDABEG genes in the presence of an autoinducer (3-oxo-hexanoyl-l-homoserine lactone). In the Aliivibrio cells, luxR genes are regulated by HNS, CRP, LitR, etc. Here we investigated the role of the luxR expression level in LuxI/R QS system functionality and improved the whole-cell biosensor for autoinducer detection. Escherichia coli-based bacterial lux-biosensors were used, in which Photorhabdus luminescensluxCDABE genes were controlled by LuxR-dependent promoters and luxR, luxR1, or luxR2 regulatory genes. We varied either the dosage of the regulatory gene in the cells using additional plasmids, or the level of the regulatory gene expression using the lactose operon promoter. It was shown that an increase in expression level, as well as dosage of the regulatory gene in biosensor cells, leads to an increase in sensitivity (the threshold concentration of AI is reduced by one order of magnitude) and to a two to threefold reduction in response time. The best parameters were obtained for a biosensor with an increased dosage of luxRA. fischeri (sensitivity to 3-oxo-hexanoyl-l-homoserine lactone reached 30–100 pM).

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“…Chan et al . have found that Aeromonas veronii biovar sobria strain 159 showed QS activities mediated by N-acyl homoserine lactone 46 . Suntharalingam et al .…”

Section: Resultsmentioning

confidence: 99%

Curcumin liposomes interfere with quorum sensing system of Aeromonas sobria and in silico analysis

Ding

Zhi

et al. 2017

Sci Rep

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Aeromonas sobria is opportunistic pathogen frequently found in environment and food. Interfering with its quorum sensing (QS) system could be a promising way to alleviate its virulence. In this study, curcumin liposomes were prepared and their characteristics like particle size, zeta potential, PDI (Polymey Disperse Index), encapsulation efficiency and loading capacity were measured. The quorum sensing inhibitory effect of curcumin liposomes under sub-MIC (Minimum Inhibitory Concentration) on siderophore production, swimming and swarming motility, extracellular proteases, biofilm formation and AHLs (N-acylhomoserine lactones) production of A. sobria were also determined. The results showed that, the curcumin liposomes with high encapsulation capacity (84.51 ± 0.58%) were stable and homogeneous. QS-regulated phenotypes of the pathogen were significantly inhibited by curcumin liposomes. The in silico analysis revealed that the QS system of A. sobria may be inhibited by released curcumin from curcumin liposomes through interacting with the built LuxI type protein and blocking the production of AHLs.

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N-Acyl Homoserine Lactone-Mediated Quorum Sensing in Aeromonas veronii biovar sobria Strain 159: Identification of LuxRI Homologs

Cited by 10 publications

References 26 publications

An Update on the Genus Aeromonas: Taxonomy, Epidemiology, and Pathogenicity

An Update on the Genus Aeromonas: Taxonomy, Epidemiology, and Pathogenicity

Influence of the luxR Regulatory Gene Dosage and Expression Level on the Sensitivity of the Whole-Cell Biosensor to Acyl-Homoserine Lactone

Curcumin liposomes interfere with quorum sensing system of Aeromonas sobria and in silico analysis

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