N-acetylmuramoyl-L-alanine amidase : A competent vaccine candidate against IPSID

Abstract

Immunoproliferative small intestine disease (IPSID) is a collective name for a range of diseases caused by various microorganisms but the major and persistent organism is Campylobacter Jejuni. IPSID can lead to minor symptoms like diarrhea, nausea, imbalance of electrolytes in the body etc. to major consequences that may lead to death in case of prolonged untreated condition. IPSID leads to infiltration of lymphocytes as a consequence of an immune response to invasion by microbes, which eventually leads to the evolvement of IgA producing bodies and to the selection of a body that produces α heavy chains. Hence, it is also called “α- Heavy chain disease”. Until now there has been no successful development of a vaccine for this disease. N-acetylmuramoyl-L-alanine amidase is one of the proteins in Campylobacter Jejuni ssp. Jejuni which is also a Potential vaccine candidate (PVC) against IPSID as identified by Vaxigen. Here, we are utilizing deep learning softwares i.e, Vaxi-DL and VaxELAN for analyzing the given protein in terms of adhesion, secretory nature, trans-membrane helices, cleavage sites, MHC-I binding, CTL epitope prediction, essential genes, molecular weight, non-bacterial pathogen, non-homology with human genome, virulence factors, allergenicity, cellular localization and probability of being a PVC.