N-acetylcysteine protects against cadmium-induced oxidative stress in rat hepatocytes

Wang, Jicang; Zhu, Huali; Liu, Zongping

doi:10.4142/jvs.2014.15.4.485

Cited by 34 publications

(21 citation statements)

References 36 publications

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“…There was significant increase in lipid oxidation byproduct, MDA level. This was the same as found by different research [25,26] in the pituitary gland and on other tissues [27][28][29].…”

Section: Discussionsupporting

confidence: 75%

Protective Effect of Montelukast against Cadmium Induced Pituitary Gland Toxicity

Ek¹,

Mf²,

Mg³

et al. 2017

IJCPT

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Adult male Sprague rats weighting (220 ± 30gm) were purchased from Faculty of Agriculture, Minia University Egypt. Experimen- AbstractCadmium (Cd) is a common environmental hazardous heavy metal. The standard treatment of heavy metal intoxication; chelators may have a minor role in Cd toxicity. Montelukast (ML) is an anti-inflammatory drug that is used mainly for managing asthmatic patients. Recent workers have tested ML for its antioxidant properties in various conditions and obtained encouraging results as regarding this effect. We investigated the protective and antioxidant effect of ML on pituitary gland, a highly sensitive organ to Cd toxicity. Four groups of rats were used and received distilled water, 10mg/kg ML, 3mg/kg Cd, and 10mg ML plus 3mg/kg Cd respectively. Antioxidant, histological, immunohistochemical and hormonal effects of Cd and ML were evaluated. The detrimental effect of Cd on pituitary gland was abolished by ML. The diminished hormonal effect of Cd was restored to normal levels. The increased MDA level and depleted oxidative enzymes on pituitary gland of rat exposed to Cd were restored by ML. lastly, histological and apoptotic effects of Cd were also, corrected significantly by ML. It is concluded that ML can protect against Cd-induced pituitary gland toxicity.

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“…There was significant increase in lipid oxidation byproduct, MDA level. This was the same as found by different research [25,26] in the pituitary gland and on other tissues [27][28][29].…”

Section: Discussionsupporting

confidence: 75%

Protective Effect of Montelukast against Cadmium Induced Pituitary Gland Toxicity

Ek¹,

Mf²,

Mg³

et al. 2017

IJCPT

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“…It has been used as a mucolytic agent as well as an antioxidant in various clinical applications for more than 30 years (Cotgreave, 1997;Radomska-Lesniewska and Skopinski, 2012). The antioxidant role of NAC in reducing Cd toxicity was extensively studied in various cell lines and animal models (Odewumi et al, 2011;Luo et al, 2013;Wang et al, 2014). However, very few studies were reported on the immunomodulatory effect of NAC in reducing Cd toxicity in liver cells (Dong et al, 1998;Souza et al, 2004) and so far, no study was reported in lung cells.…”

Section: Introductionmentioning

confidence: 99%

Modulation of cytokines and chemokines expression by NAC in cadmium chloride treated human lung cells

Odewumi

Latinwo

Ruden

et al. 2015

Environmental Toxicology

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Cadmium (Cd), is one of the most hazardous metals found in the environment. Cd exposure through inhalation has been linked to various diseases in lungs. It was shown that Cd induces pro-inflammatory cytokines through oxidative stress mechanism. In this report, we studied the immuno-modulatory effect of a well known antioxidant, N-acetylcysteine (NAC) on cadmium chloride (CdCl2) treated human lung A549 cells through human cytokine array 6. The lung cells were treated with 0, or 75 μM CdCl2 alone, 2.5 mM NAC alone, or co-treated with 2.5 mM NAC and 75 μM CdCl2 for 24 h. The viability of cells was measured by crystal violet dye. The array results were validated by human IL-1alpha enzyme-linked immunosorbent assay (ELISA) kit. The viability of the 75 μM CdCl2 alone treated cells was decreased to 44.5%, while the viability of the co-treated cells with 2.5 mM NAC was increased to 84.1% in comparison to untreated cells. In the cell lysate of CdCl2 alone treated cells, 19 and 8 cytokines were up and down-regulated, while in the medium 15 and 3 cytokines were up and down-regulated in comparison to the untreated cells. In the co-treated cells, all these cytokines expression was modulated by the NAC treatment. The IL-1α ELISA result showed the same pattern of cytokine expression as the cytokine array. This study clearly showed the modulatory effect of NAC on cytokines and chemokines expression in CdCl2-treated cells and suggests the use of NAC as protective agent against cadmium toxicity.

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“…The protective effects of NAC are evident in chronic diseases where decreased GSH or oxidative stress such as NASH occur (Ronis et al, 2005). NAC, as a source of sulfhydryl groups, stimulates GSH synthesis, enhances GSH-S-transferase (GST) activity and acts as a scavenger of free radicals, thus getting rid of ROS (Wang et al, 2014). It was selected in the current study as a representative of synthetic antioxidants, as it was welldocumented as a safe beneficial agent in a variety of pathologies (Rushworth & Megson, 2014).…”

Section: Introductionmentioning

confidence: 99%

Protective effect of ursodeoxycholic acid, resveratrol, andN-acetylcysteine on nonalcoholic fatty liver disease in rats

Ali

Messiha

Abdellatif

2015

Pharmaceutical Biology

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(2016) Protective effect of ursodeoxycholic acid, resveratrol, and Nacetylcysteine on nonalcoholic fatty liver disease in rats, Pharmaceutical Biology, 54:7, 1198-1208, DOI: 10.3109/13880209.2015 -a), glucose, albumin, renal functions (urea, creatinine), lipid profile (total cholesterol; TC, triglycerides, high density lipoproteins, low density lipoproteins; LDL-C, very low density lipoproteins, leptin), and oxidative stress markers (hepatic malondialdehyde; MDA, glutathione; GSH, glutathione-S-transferase; GST) were measured using automatic analyzer, colorimetric kits, and ELISA kits, supported by a liver histopathological study.Results: RSV and NAC administration significantly improved liver index (RSV only), alanine transaminase (52, 52%), TNF-a (70, 70%), glucose (69, 80%), albumin (122, 114%), MDA (55, 63%), GSH (160, 152%), GST (84, 84%), TC (86, 86%), LDL-C (83, 81%), and leptin (59, 70%) levels compared with steatosis control values. A combination of RSV or NAC with UDCA seems to ameliorate their effects. Discussion and conclusion: RSV and NAC are effective on NAFLD through antioxidant, antiinflammatory, and lipid-lowering potentials, where as RSV seems better than UDCA or NAC.

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N-acetylcysteine protects against cadmium-induced oxidative stress in rat hepatocytes

Cited by 34 publications

References 36 publications

Protective Effect of Montelukast against Cadmium Induced Pituitary Gland Toxicity

Protective Effect of Montelukast against Cadmium Induced Pituitary Gland Toxicity

Modulation of cytokines and chemokines expression by NAC in cadmium chloride treated human lung cells

Protective effect of ursodeoxycholic acid, resveratrol, andN-acetylcysteine on nonalcoholic fatty liver disease in rats

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