N-acetylcysteine protect inner hair cells from cisplatin by alleviated celluar oxidative stress and apoptosis

Wang, Weilong; Chen, Erfang; Ding, Xuerui; Lu, Pei-Heng; Chen, Jiawei; Ma, Peng-Wei; Lu, Lianjun

doi:10.1016/j.tiv.2022.105354

Cited by 11 publications

(4 citation statements)

References 40 publications

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“…Moreover, the inner ear was a high energy consumption organ whose demands on energy supply were enormous ( Chen et al, 2022c ). When glucose metabolism cannot meet the energy supply in the inner ear under physiological or pathological conditions, fatty acid oxidation will be enhanced ( Chen et al, 2022d ; Wang et al, 2022 ). Previous studies suggested that fatty acid metabolism was disturbed when inner hair cells exposing to pathophysiological stimuli; cumulated fatty acids could facilitate oxidative stress injury and apoptosis in the inner hair cells, resulting in acute hearing loss ( Fujita et al, 2015 ; Pirttilä et al, 2019 ; He et al, 2022 ; Peter et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Diagnostic and predictive values of serum metabolic profiles in sudden sensorineural hearing loss patients

Wang

Gao

Jiang

2022

Front. Mol. Biosci.

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Sudden sensorineural hearing loss (SSNHL) is an otologic emergency, and metabolic disturbance is involved in its pathogenesis. This study recruited 20 SSNHL patients and 20 healthy controls (HCs) and collected their serum samples. Serum metabolites were detected by liquid chromatography-mass spectrometry, and metabolic profiles were analyzed. All patients were followed up for 3 months and categorized into recovery and non-recovery groups. The distinctive metabolites were assessed between two groups, and their predictive values for hearing recovery were evaluated. Analysis results revealed that SSNHL patients exhibited significantly characteristic metabolite signatures compared to HCs. The top 10 differential metabolites were further analyzed, and most of them showed potential diagnostic values based on receiver operator characteristic (ROC) curves. Finally, 14 SSNHL patients were divided into the recovery group, and six patients were included in the non-recovery group. Twelve distinctive metabolites were observed between the two groups, and ROC curves demonstrated that N4-acetylcytidine, p-phenylenediamine, sphingosine, glycero-3-phosphocholine, and nonadecanoic acid presented good predictabilities in the hearing recovery. Multivariate analysis results demonstrated that serum N4-Acetylcytidine, sphingosine and nonadecanoic acid levels were associated with hearing recovery in SSNHL patients. Our results identified that SSNHL patients exhibited distinctive serum metabolomics signatures, and several serum biomarkers were proved to be potential in predicting hearing recovery. The discriminative metabolites might contribute to illustrating the mechanisms of SSNHL and provide possible clues for its treatments.

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Section: Discussionmentioning

confidence: 99%

Diagnostic and predictive values of serum metabolic profiles in sudden sensorineural hearing loss patients

Wang

Gao

Jiang

2022

Front. Mol. Biosci.

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“…N-acetylcysteine (NAC), a thiol-containing antioxidant, is often used as a direct scavenger of reactive oxygen species, especially hydrogen peroxide, and has shown substantial renoprotective activity ( Kalyanaraman, 2022 , Wang et al, 2022 ). Therefore, NAC was selected as the positive control drug in this study.…”

Section: Methodsmentioning

confidence: 99%

Exploring the chemical composition, medicinal benefits, and antioxidant activity of Plumula nelumbinis essential oil from different habitats in China

Huang,

Wang,

Xie

et al. 2023

Saudi Pharmaceutical Journal

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“…The organ of Corti was dissected from P3 ICR mice as previously reported. 25 The sterilized 1% PVA/Gel/SA scaffolds were put into the 24-well cell culture plate, and 1 mL culture medium (DMEM/F12 + 10% FBS + 1% P/S) was added to the well overnight. Next, the culture medium were removed and fresh medium were added until the scaffolds were immersed.…”

Section: Construction and Evaluation Of Organ Of Corti Organoidsmentioning

confidence: 99%

Construction of organ of Corti organoid to study the effects of berberine sulfate on damaged auditory cells

Zhang,

Liu,

Shen

et al. 2024

J Biomed Mater Res

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Sensorineural hearing loss (SNHL) is mainly caused by injury or loss of hair cells (HCs) and associated spiral ganglion neurons (SGNs) in the inner ear. At present, there is still no effective treatment for SNHL in clinic. Recently, advances in organoid bring a promising prospect for research and treatment of SNHL. Meanwhile, three‐dimensional (3D) printing provides a tremendous opportunity to construct versatile organoids for tissue engineering and regenerative medicine. In this study, gelatin (Gel), sodium alginate (SA), and polyvinyl alcohol (PVA) were used to fabricate biomimetic scaffold through 3D printing. The organ of Corti derived from neonatal mice inner ear was seeded on the PVA/Gel/SA scaffold to construct organ of Corti organoid. Then, the organ of Corti organoid was used to study the potential protective effects of berberine sulfate on neomycin‐juried auditory HCs and SGNs. The results showed that the PVA/Gel/SA biomimetic 3D scaffolds had good cytocompatibilities and mechanical properties. The constructed organoid could maintain organ of Corti activity well in vitro. In addition, the injury intervention results showed that berberine sulfate could significantly inhibit neomycin‐induced HC and SGN damage. This study suggests that the fabricated organoid is highly biomimetic to the organ of Corti, which may provide an effective model for drug development, cell and gene therapy for SNHL.

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N-acetylcysteine protect inner hair cells from cisplatin by alleviated celluar oxidative stress and apoptosis

Cited by 11 publications

References 40 publications

Diagnostic and predictive values of serum metabolic profiles in sudden sensorineural hearing loss patients

Diagnostic and predictive values of serum metabolic profiles in sudden sensorineural hearing loss patients

Exploring the chemical composition, medicinal benefits, and antioxidant activity of Plumula nelumbinis essential oil from different habitats in China

Construction of organ of Corti organoid to study the effects of berberine sulfate on damaged auditory cells

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