N-Acetylcysteine Attenuates Iodine Contrast Agent-Induced Nephropathy in 5/6-Nephrectomized Rats

Šochman, J; Peregrin, J; Bürgelová, Marcela; Kopkan, Libor; Kramer, Herbert J.; Červenka, Luděk

doi:10.1159/000315435

Cited by 13 publications

(10 citation statements)

References 93 publications

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“…Therefore, various blood flow-increasing agents were clinically tested to prevent CIN; however, most of these have been reported to be ineffective [2]. In the experimental and/or clinical studies, caffeic acid phenyl ester (CAPE), N-acetylcysteine (NAC) and ascorbic acid have been reported to have positive effects in preventing CIN [7,11,12,13,14]. For this purpose, oxidative stress, rather than decrease in renal blood flow, is considered to play an important role in CIN development [2].…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of the Grape Seed Proanthocyanidin Extract in a Rat Model of Contrast-Induced Nephropathy

Özkan

Ulusoy

Örem

et al. 2012

Kidney Blood Press Res

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Aim: Contrast-induced nephropathy (CIN) is a common cause of hospital-acquired acute renal failure. Although it is so common, there has been no approved therapy yet. We aimed to investigate the effect of grape seedproanthocyanidin extract (GSPE) on preventing CIN. Materials and Methods: 24 rats were divided into four groups as control group, GSPE group, contrast medium (CM) group, and CM+GSPE group. The experiment was discontinued on the ninth day. Blood samples were obtained for the measurement of renal function parameters. Renal tissues of the rats were removed for the analysis of oxidative system parameters. In addition to renal histopathology, transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL) was performed to determine apoptosis. Results: There was a significant increase in BUN, creatinine, malondialdehyde (MDA) levels, apoptotic index(AI)and histopathological alteration in the CM group as compared to the control group. Furthermore, BUN, creatinine, MDA, total oxidant system and oxidative stress index levels, AI as well as renal histopathological alteration were significantly decreased in the CM+GSPE group. Conclusion: For the first time in the literature, we showed that GSPE provided biochemical and histopathological improvement in CIN. Our findings revealed that this improvement was associated with the decrease in oxidative damage and apoptosis.

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Section: Discussionmentioning

confidence: 99%

Protective Effect of the Grape Seed Proanthocyanidin Extract in a Rat Model of Contrast-Induced Nephropathy

Özkan

Ulusoy

Örem

et al. 2012

Kidney Blood Press Res

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“…On day 0, 5 ⁄ 6 NX was carried out under anaesthesia (tiletamine + zolazepam; Virbac SA, Carros Cedex, France; 8 mg ⁄ kg; and xylasine, Spofa, Czech Republic; 4 mg ⁄ kg intramuscularly), as described previously. 3,7,8,13,17,18,20,43,44 Briefly, an abdominal midline incision was carried out to expose the kidneys. The right kidney and both poles of the left kidney were removed surgically in order to remove 5 ⁄ 6 of renal parenchyma, as estimated by kidney weight.…”

Section: Experimental Protocolsmentioning

confidence: 99%

“…This approach was previously validated in our laboratory and is regularly used in our studies. 27,29,40,43,44 Series 2: Effects of RAS-dependent and RAS-independent antihypertensive therapy on plasma and kidney AngII levels, cardiac hypertrophy, renal glomerular damage and kidney tubulointerstitial injury Animals were divided into the following experimental groups and were exposed to the same experimental protocol as in series 1:…”

Section: Experimental Protocolsmentioning

confidence: 99%

Similar renoprotection after renin‐angiotensin‐dependent and ‐independent antihypertensive therapy in 5/6‐nephrectomized Ren‐2 transgenic rats: are there blood pressure‐independent effects?

Kujal

Chábová

Vernerová³

et al. 2010

Clin Exp Pharma Physio

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1. Hypertension plays a critical role in the progression of chronic kidney disease (CKD) to end-stage renal disease (ESRD), but it has also been postulated that antihypertensive drugs that block the renin-angiotensin system (RAS) show class-specific renoprotective actions beyond their blood pressure (BP)-lowering effects. 2. Because this notion has recently been questioned, in the present study we compared the effects of a RAS-dependent antihypertensive therapy (a combination of trandolapril, an angiotensin-converting enzyme inhibitor (ACEI) and losartan, an angiotensin-II (AngII) receptor subtype 1A receptor antagonist) with a 'RAS-independent' antihypertensive therapy (a combination of labetalol, an alfa- and beta-adrenoreceptor antagonist with the diuretics, hydrochlorothiazide and furosemide) on the progression of CKD after 5/6 renal ablation (5/6 NX) in Ren-2 renin transgenic rats (TGR), a model of AngII-dependent hypertension. Normotensive transgene-negative Hannover Sprague-Dawley (HanSD) rats after 5/6 NX served as controls. 3. RAS-dependent and -independent antihypertensive therapies normalized BP and survival rate, and prevented the development of cardiac hypertrophy and glomerulosclerosis to the same degree in 5/6 NX HanSD rats and in 5/6 NX TGR. The present findings show that renoprotection, at least in rats after 5/6 NX, is predominantly BP-dependent. When equal lowering of BP was achieved, leading to normotension, cardio- and renoprotective effects were equivalent irrespective of the type of antihypertensive therapy. 4. These findings should be taken into consideration in attempts to develop new therapeutic approaches and strategies aimed to prevent the progression of CKD and to lower the incidence of ESRD.

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“…To evaluate the renoprotective effects of various compounds, the experimental AKI rat model is useful and the results can be extrapolated to clinical AKI (4,31,36). Few compounds have been used in clinical trials and found to have therapeutic value.…”

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confidence: 99%

Sesame Oil Prevents Acute Kidney Injury Induced by the Synergistic Action of Aminoglycoside and Iodinated Contrast in Rats

Hsu

Chu

et al. 2011

Antimicrob Agents Chemother

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The aim of the study was to investigate the effect of sesame oil on acute kidney injury induced by the synergistic action of aminoglycoside and iodinated contrast in rats. Acute kidney injury was induced by a 5-day course of daily gentamicin injections (100 mg/kg of body weight, subcutaneously) and then iodinated contrast (4 ml/kg, intravenously) in male specific-pathogen-free Sprague-Dawley rats. Sesame oil (0.5 ml/kg, orally) was given 1 h before iodinated contrast. Renal function and oxidative stress were assessed 6 h after iodinated contrast injection. Renal function was evaluated by measuring serum blood urea nitrogen and creatinine levels. Renal oxidative stress was assessed by determining renal lipid peroxidation, myeloperoxidase, hydroxyl radical, superoxide anion, nitrite/nitrate, and inducible nitric oxide synthase levels. Sesame oil significantly prevented the rise of serum blood urea nitrogen and creatinine levels. Furthermore, there was a parallel inhibition of the rise in levels of expression of renal lipid peroxidation, myeloperoxidase, hydroxyl radicals, superoxide anion, nitrite/nitrate, and inducible nitric oxide synthase in rats with gentamicin-plus-iodinated contrast-induced acute kidney injury. We conclude that sesame oil may attenuate aminoglycoside-plus-iodinated contrast-induced acute kidney injury by inhibiting renal oxidative stress in rats.The development of radiologic contrast media originated with the use of iodinated contrast medium in radiography in 1918 (18). The use of iodinated contrast medium has been increasing along with the increasing use of diagnostic imaging. It is estimated that more than 80 million patients received iodinated contrast medium in 2003 worldwide (21). Because iodinated contrast is widely used, it is sometimes administered to patients receiving an aminoglycoside. The action of this combination of contrast agents and aminoglycosides against renal hemodynamics and tubular cell function is potentially synergistic (25). In addition, a large cohort study has found that the use of iodinated contrast significantly increased the risk of acute kidney injury (AKI) in patients treated with aminoglycosides (25).Oxidative stress plays an important role in the pathogenesis of iodinated contrast-associated AKI in renally compromised patients (24). The vasoconstriction and renal ischemia caused by contrast media leads to the generation of reactive oxygen species (ROS) and nitric oxide, both of which are involved in the development of renal oxidative stress and tubular cell damage (1,2,20,24). Therefore, inhibiting contrast-associated oxidative stress may be beneficial in preventing iodinated contrast-plus-aminoglycoside-induced renal injury. Sesame oil is a potent antioxidative agent (9)(10)(11)14). Sesame oil potently reduces renal oxidative stress by inhibiting the generation of ROS and nitric oxide in septic rats (9)(10)(11)14). Further, sesame oil protects against gentamicin-induced renal injury by inhibiting renal oxidative stress in rats (17). However, the effect of s...

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N-Acetylcysteine Attenuates Iodine Contrast Agent-Induced Nephropathy in 5/6-Nephrectomized Rats

Cited by 13 publications

References 93 publications

Protective Effect of the Grape Seed Proanthocyanidin Extract in a Rat Model of Contrast-Induced Nephropathy

Protective Effect of the Grape Seed Proanthocyanidin Extract in a Rat Model of Contrast-Induced Nephropathy

Similar renoprotection after renin‐angiotensin‐dependent and ‐independent antihypertensive therapy in 5/6‐nephrectomized Ren‐2 transgenic rats: are there blood pressure‐independent effects?

Sesame Oil Prevents Acute Kidney Injury Induced by the Synergistic Action of Aminoglycoside and Iodinated Contrast in Rats

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