N-acetylcysteine attenuates dimethylnitrosamine induced oxidative stress in rats

Sathish, Priya; Vijayalakshmi, P.; Vivekanandan, Perumal; Karthikeyan, S.

doi:10.1016/j.ejphar.2010.10.080

Cited by 18 publications

(6 citation statements)

References 40 publications

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“…NAC was chosen over other reactive oxidants scavengers because of its strong efficiency to enter inside cells and less toxicity in comparison with other reactive oxidant scavengers. NAC has been used as a reactive oxidant scavenger in vivo (91,92). Neither DFO nor NAC reduced the parasite burden at the dose used indicating that the hepatoprotective effect of DFO and NAC was not due to any antiparasitic effect.…”

Section: Discussionmentioning

confidence: 99%

Impact of Intravascular Hemolysis in Malaria on Liver Dysfunction

Dey¹,

Bindu

Goyal

et al. 2012

Journal of Biological Chemistry

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Section: Discussionmentioning

confidence: 99%

Impact of Intravascular Hemolysis in Malaria on Liver Dysfunction

Dey¹,

Bindu

Goyal

et al. 2012

Journal of Biological Chemistry

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“…NAC administration in a CCl 4 -inhaled cirrhotic rat model resulted in a lesser decrease in GPx levels and collagen deposition with a lower degree of fibrosis than those of only CCl 4 -treated counterparts [39]. Post-treatment with NAC protected rat livers from NDMA-induced oxidative stress and hepatocellular damage, restoring the activities of both the enzymatic and nonenzymatic antioxidants [40]. Restoring GSH levels in the livers of O. felineus-infected hamsters under NAC uptake in drinking water allowed the activation of GPx and GST as well as decreases in collagen content and liver fibrosis severity [41].…”

Section: Discussionmentioning

confidence: 92%

The Overactivation of NADPH Oxidase during Clonorchis sinensis Infection and the Exposure to N-Nitroso Compounds Promote Periductal Fibrosis

Jeong

Hwang

et al. 2021

Antioxidants

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Clonorchis sinensis, a high-risk pathogenic human liver fluke, provokes various hepatobiliary complications, including epithelial hyperplasia, inflammation, periductal fibrosis, and even cholangiocarcinogenesis via direct contact with worms and their excretory–secretory products (ESPs). These pathological changes are strongly associated with persistent increases in free radical accumulation, leading to oxidative stress-mediated lesions. The present study investigated C. sinensis infection- and/or carcinogen N-nitrosodimethylamine (NDMA)-associated fibrosis in cell culture and animal models. The treatment of human cholangiocytes (H69 cells) with ESPs or/and NDMA increased reactive oxidative species (ROS) generation via the activation of NADPH oxidase (NOX), resulting in augmented expression of fibrosis-related proteins. These increased expressions were markedly attenuated by preincubation with a NOX inhibitor (diphenyleneiodonium chloride) or an antioxidant (N-acetylcysteine), indicating the involvement of excessive NOX-dependent ROS formation in periductal fibrosis. The immunoreactive NOX subunits, p47phox and p67phox, were observed in the livers of mice infected with C. sinensis and both infection plus NDMA, concomitant with collagen deposition and immunoreactive fibronectin elevation. Staining intensities are proportional to lesion severity and infection duration or/and NDMA administration. Thus, excessive ROS formation via NOX overactivation is a detrimental factor for fibrogenesis during liver fluke infection and exposure to N-nitroso compounds.

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“…There is abundant evidence regarding the use of NAC as an antioxidant. It is a thiol-containing molecule that has been widely used for promoting GSH synthesis (Moldeus et al 1986; Sathish et al 2011; Atkuri et al 2007). The rationale for the use of NAC in the present study was that it would be able to protect against the deleterious effects of TeAsp.…”

Section: Discussionmentioning

confidence: 99%

Sub-acute administration of (S)-dimethyl 2-(3-(phenyltellanyl) propanamido) succinate induces toxicity and oxidative stress in mice: unexpected effects of N-acetylcysteine

et al. 2013

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The organic tellurium compound (S)-dimethyl 2-(3-(phenyltellanyl) propanamide) succinate (TeAsp) exhibits thiol-peroxidase activity that could potentially offer protection against oxidative stress. However, data from the literature show that tellurium is a toxic agent to rodents. In order to mitigate such toxicity, N-acetylcysteine (NAC) was administered in parallel with TeAsp during 10 days. Mice were separated into four groups receiving daily injections of (A) vehicle (PBS 2.5 ml/kg, i.p. and DMSO 1 ml/kg, s.c.), (B) NAC (100 mg/kg, i.p. and DMSO s.c.), (C) PBS i.p. and TeAsp (92.5 μmol/kg, s.c), or (D) NAC plus TeAsp. TeAsp treatment started on the fourth day. Vehicle or NAC-treated animals showed an increase in body weight whereas TeAsp caused a significant reduction. Contrary to expected, NAC co-administration potentiated the toxic effect of TeAsp, causing a decrease in body weight. Vehicle, NAC or TeAsp did not affect the exploratory and motor activity in the open-field test at the end of the treatment, while the combination of NAC and TeAsp produced a significant decrease in these parameters. No DNA damage or alterations in cell viability were observed in leukocytes of treated animals. Treatments produced no or minor effects on the activities of antioxidant enzymes catalase, glutathione peroxidase and glutathione reductase, whereas the activity of the thioredoxin reductase was decreased in the brain and increased the liver of the animals in the groups receiving TeAsp or TeAsp plus NAC. In conclusion, the toxicity of TeAsp was potentiated by NAC and oxidative stress appears to play a central role in this process.Electronic supplementary materialThe online version of this article (doi:10.1186/2193-1801-2-182) contains supplementary material, which is available to authorized users.

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N-acetylcysteine attenuates dimethylnitrosamine induced oxidative stress in rats

Cited by 18 publications

References 40 publications

Impact of Intravascular Hemolysis in Malaria on Liver Dysfunction

Impact of Intravascular Hemolysis in Malaria on Liver Dysfunction

The Overactivation of NADPH Oxidase during Clonorchis sinensis Infection and the Exposure to N-Nitroso Compounds Promote Periductal Fibrosis

Sub-acute administration of (S)-dimethyl 2-(3-(phenyltellanyl) propanamido) succinate induces toxicity and oxidative stress in mice: unexpected effects of N-acetylcysteine

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