N-Acetyl-Serotonin Protects HepG2 Cells from Oxidative Stress Injury Induced by Hydrogen Peroxide

Jiang, Jiying; Yu, Shanshan; Jiang, Zhengchen; Liang, Cuihong; Yu, Wenbo; Li, Jin; Du, Xiaodong; Wang, Hailiang; Gao, Xianghong; Wang, Xin

doi:10.1155/2014/310504

Cited by 63 publications

(44 citation statements)

References 93 publications

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“…The activity and translation of SOD in the model group was higher than the untreated group. This is contrary to the results of Jiang et al (31). The reason for this might be that the accumulation of fat in the model cells could not induce stress enough to reduce SOD.…”

Section: Discussioncontrasting

confidence: 93%

Influence of different concentrations of uric acid on oxidative stress in steatosis hepatocytes

Cheng

Yang²,

Zhou

et al. 2018

Exp Ther Med

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Abstract. The development of nonalcoholic fatty liver disease (NAFLD) is caused by the steatosis of hepatocytes, which induces oxidative stress (OS). Thus, OS has an important role in the development of NAFLD. In the present study, the L-02 hepatocyte cell line was used to develop a steatosis cell model. The best model was determined using an MTT assay and the triglyceride levels. Model cells were treated with high concentrations of uric acid (UA; 0, 5, 10, 20 and 30 mg/dl) for 24, 48, 72 and 96 h. Indicators of oxidation were then measured, which included total superoxide dismutase (SOD), malonaldehyde (MDA) and reduced glutathione (GSH), and the transcriptional and translational levels of SOD1 and γ-glutamate-cysteine ligase (γ-GCLC) were also determined. In addition, the intracellular levels of aspartate aminotransferase and alanine aminotransferase (ALT) were detected. The activity of SOD1 decreased over time and the result was supported by the results of western blotting. The transcriptional levels of SOD1 in model cells was significantly higher than untreated cells at 48 h. With the decreased levels of SOD1 and GSH, MDA increased in a time-dependent manner. The content of GSH decreased with time as well, which was also reflected in the results of western blotting. The transcriptional levels of γ-GCLC in all UA-treated groups were lower when compared with those observed in the model group. The activity of ALT tended to increase, depending on the duration of treatment. Treatment with 5 and 10 mg/dl UA had an antioxidative effect on the model cells, and 30 mg/dl UA treatment for 48 h increased OS in the cells.

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Section: Discussioncontrasting

confidence: 93%

Influence of different concentrations of uric acid on oxidative stress in steatosis hepatocytes

Cheng

Yang²,

Zhou

et al. 2018

Exp Ther Med

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“…Therefore we exposed HepG2 cells to a concentration of 30 µM H2O2 for 2 h to induce oxidative stress for further experiments. To evaluate the most effective concentration of liquorice, HepG2 cells were treated with different concentration of liquorice (10-100 µg), and found cell viability decreased by about 50% [15] at the concentration of 60 µg fig. 3(B) which was used for further experiments.…”

Section: Resultsmentioning

confidence: 99%

“…Cells passaging was done every third day after cells were at least 80% confluent as per the method described by Jiying J. et al, 2014 [15]. Cells were incubated in a humidified atmosphere and 5% CO2…”

Section: Maintenance Of Cell Linementioning

confidence: 99%

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Antioxidant Activity and Antiproliferative Action of Methanolic Extract of Liquorice (Glycyrrhiza Glabra) in Hepg2 Cell Line

Shinde

Koratkar²,

Sharma³

et al. 2016

Int J Pharm Pharm Sci

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Objective: To evaluate the in vitro antioxidant activity of liquorice (Glycyrrhiza glabra) against H2O2Methods: Antioxidant activity of methanolic extracts of Glycyrrhiza glabra was investigated by measuring total phenolic content using folinciocalteu reagent (FCR), free radical scavenging activity by DPPH and ferric reducing antioxidant power (FRAP). The presence of phenolic compounds and flavonoids in the extract was confirmed by Liquid Chromatography-Mass Spectrometry (LC-MS) analysis. Furthermore, the protective effect of methanolic extract of Glycyrrhiza glabra against oxidative stress induced by H induced oxidative stress in HepG2 cell line.2 O2 in HepG2 cells was investigated by MTT assay. HepG2 cells were exposed with five different treatments viz. liquorice, H2O2, ascorbic acid, H2O2+liquorice and H2O2 Results:The total phenolic content estimated in Glycyrrhiza glabra extract was found to be 241.47 µg per 1000 µg/ml of methanolic extract. It was found that as the concentration of the extract was increased both the free radical scavenging activity and ferric ion reducing power was also found to increase. LC-MS analysis confirmed the presence of eight different phenolic compounds in the methanolic extract which are possibly contributing to the antioxidant activity exhibited by the extract. It was also observed that liquorice treated HepG2 cells showed lower MDA and higher glutathione and catalase levels as compared to only H +ascorbic acid, to explore the effect of the extract on malondialdehyde (MDA) production, catalase activity, and glutathione reductase levels. 2O 2 Conclusion:Our results thus conclude that, the methanolic extract of Glycyrrhiza glabra can be used as natural supplements in various disease conditions where oxidative stress has been reported. treated HepG2 cells where increased MDA production, decreased glutathione reductase and catalase production was observed.

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“…It is stable and can be converted into a hydroxyl radical, the most destructive free radicals. It is also more cell membrane permeable than other ROS (Jiang et al, 2014). It induces cell damage by propagation of inflammation, apoptosis and cell death.…”

Section: Discussionmentioning

confidence: 99%

Diazoxide preconditioning of endothelial progenitor cells improves their ability to repair the infarcted myocardium

Mehmood

Muhammad

Khan

et al. 2015

Cell Biology International

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Reduced survival and homing of the transplanted cells in the oxidative stressed ischemic environment limits the potential outcome of cell therapies for myocardial ischemia. Diazoxide (DZ), a highly selective mitochondrial ATP sensitive K(+) channel opener, is known to improve the survival and therapeutic ability of mesenchymal stem cells and skeletal myoblasts for the repair of heart failure. The current study explored the effect of DZ preconditioning in improving the ability of endothelial progenitor cells (EPCs) to counteract, in vitro oxidative stress, and to repair the infarcted myocardium. The EPCs were preconditioned by 30 min incubation with 200 μM DZ followed by exposure to 200 μM hydrogen peroxide (H2 O2 ) for 2 h. Non-preconditioned EPCs with and without exposure to H2 O2 were used as control. DZ preconditioning of EPCs resulted in significantly reduced cell injury as shown by reduced lactate dehydrogenase release and expression of annexin V-PE in comparison to untreated EPCs. Furthermore, DZ preconditioned EPCs exhibited upregulated expression of prosurvival genes (VEGF, SDF-1α, PCNA, and Bcl2 ), improved chemokines release (VEGF, IGF, and SDF-1α), viability, Akt phosphorylation and tube formation. In vivo experiments involved transplantation of DZ preconditioned and untreated EPCs in the left ventricle after permanent ligation of left anterior descending coronary artery in rats. The results demonstrated that DZ EPCs transplanted group showed significant reduction in infarct size along with robust cell proliferation, angiogenesis and improvement in cardiac function. The current study demonstrates that DZ preconditioning enhances EPCs survival under oxidative stress in vitro and their ability to treat myocardial infarction.

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N-Acetyl-Serotonin Protects HepG2 Cells from Oxidative Stress Injury Induced by Hydrogen Peroxide

Cited by 63 publications

References 93 publications

Influence of different concentrations of uric acid on oxidative stress in steatosis hepatocytes

Influence of different concentrations of uric acid on oxidative stress in steatosis hepatocytes

Antioxidant Activity and Antiproliferative Action of Methanolic Extract of Liquorice (Glycyrrhiza Glabra) in Hepg2 Cell Line

Diazoxide preconditioning of endothelial progenitor cells improves their ability to repair the infarcted myocardium

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