N-acetyl-l-cysteine protects porcine oocytes undergoing meiotic resumption from heat stress

Hu, Xiao; Cheng, Linghua; Wang, Xiaodong; Luo, Gang; Zhao, Tianqing; Tian, Jianhui; An, Liguo

doi:10.1016/j.reprotox.2019.10.006

Cited by 16 publications

(10 citation statements)

References 53 publications

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“…To test this possibility, we firstly assessed the incidence of PBE and cumulus cell expansion, two obvious morphological indicators for the porcine oocyte meiosis. Consistent with previous reports, heat stress impaired the meiotic maturation of porcine oocytes in vitro [ 34 , 35 ]. Whereas NMN supplementation elevated the rate of PBE and the degree of cumulus cell expansion, validating the beneficial effects of NMN on the oocyte maturation under heat stress.…”

Section: Discussionsupporting

confidence: 93%

Nicotinamide mononucleotide supplementation improves the quality of porcine oocytes under heat stress

Song

Liu

Zhou

et al. 2022

J Animal Sci Biotechnol

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Background Elevated ambient temperature-caused heat stress is a major concern for livestock production due to its negative impact on animal feed intake, growth, reproduction, and health. Particularly, the germ cells are extremely sensitive to the heat stress. However, the effective approach and strategy regarding how to protect mammalian oocytes from heat stress-induced defects have not been determined. Methods Germinal vesicle (GV) porcine oocytes were cultured at 41.5 °C for 24 h to induce heat stress, and then cultured at 38.5 °C to the specific developmental stage for subsequent analysis. Nicotinamide mononucleotide (NMN) was dissolved in water to 1 mol/L for a stock solution and further diluted with the maturation medium to the final concentrations of 10 μmol/L, 20 μmol/L, 50 μmol/L or 100 μmol/L, respectively, during heat stress. Immunostaining and fluorescence intensity quantification were applied to assess the effects of heat stress and NMN supplementation on the key processes during the oocyte meiotic maturation. Results Here, we report that NMN supplementation improves the quality of porcine oocytes under heat stress. Specifically, we found that heat stress resulted in oocyte maturation failure by disturbing the dynamics of meiotic organelles, including the cytoskeleton assembly, cortical granule distribution and mitochondrial function. In addition, heat stress induced the production of excessive reactive oxygen species (ROS) and DNA damage, leading to the occurrence of apoptosis in oocytes and subsequent embryonic development arrest. More importantly, we validated that supplementation of NMN during heat stress restored the meiotic defects during porcine oocyte maturation. Conclusions Taken together, our study documents that NMN supplementation is an effective approach to improve the quality of oocytes under heat stress by promoting both nuclear and cytoplasmic maturation.

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Section: Discussionsupporting

confidence: 93%

Nicotinamide mononucleotide supplementation improves the quality of porcine oocytes under heat stress

Song

Liu

Zhou

et al. 2022

J Animal Sci Biotechnol

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“…once reported that the negative effect of HS on oocytes maturation and developmental potential depended on the stages of oocytes maturation and process time. Consistent with this finding, Hu et al (2020) indicated that the oocytes were more sensitive to the environmental temperature when they were undergoing meiotic resumption. Based on these studies and our preliminary experimental results, we established an experimental model with a stage-specific HS treatment: COCs were cultured at 38.5°C or 42°C for 4 hr (20-24 hr) (Figure 1), at which the majority of oocytes were undergoing GVBD, followed by another 20 hr of maturation subsequently at 38.5°C.…”

Section: Discussionsupporting

confidence: 69%

“…The reproductive performance of mammals is more sensitive and is generally affected by climatic conditions (Hu et al., 2020). Heat stress (HS) is a major problem that affects the livestock industry worldwide causing reproductive complications (Do et al., 2015).…”

Section: Introductionmentioning

confidence: 99%

Idebenone relieves the damage of heat stress on the maturation and developmental competence of porcine oocytes

Shi

Jin

Lin

et al. 2022

Reprod Domestic Animals

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The reproductive function of animals is often affected by climatic conditions. High‐temperature conditions can cause damage to oocyte maturation and embryonic development in a variety of ways. The purpose of this study was to prove that supplementation idebenone (IDB) to the maturation medium can improve the maturation and development of porcine oocytes after heat stress (HS). Porcine cumulus‐oocyte complexes (COCs) were cultured in the maturation medium with different concentrations of IDB (0, 0.1, 1 and 10 μM) for 44 hr at either 38.5°C or under the HS conditions. The cumulus oophorus expansion, nuclear maturation and blastocyst rate after parthenogenetic activation (PA) were measured. We found that HS (in vitro maturation 20–24 hr, 42°C) exposure significantly reduced cumulus expansion index and maturation rate of oocytes and the blastocyst rate of PA embryos, while IDB supplementation significantly improved oocyte maturation and development to the blastocysts stage after PA. Moreover, the addition of IDB decreased the intracellular level of ROS and increased GSH content, hence enhancing the antioxidant capacity of oocytes under HS. Meanwhile, IDB treatment also obviously improved the mitochondrial membrane potential and ATP synthesis of oocytes under HS conditions. Furthermore, IDB treatment increased the expression of GDF9 and BMP15 in IVM oocytes which attribute to improve the quality and outcome of IVM oocytes and the development competence of PA embryos in pigs. In summary, we demonstrated that IDB supplementation into the maturation medium exerted protective effects and improved the ability of maturation and developmental competence of porcine oocytes exposed to HS.

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“…Moreover, results from animal studies have confirmed that NAC is neither teratogenic nor mutagenic [22] and can be safely used in humans [23]. Previous studies showed that treatment with 1.0 mM NAC in vivo could delay oocyte aging in mice [24,25] and protect porcine oocytes against the oxidative stress and apoptosis induced by heat stress [26], as well as increase SOD activity by reducing the degree of DNA fragmentation [27]. However, up until now, there have been limited studies regarding the beneficial effects of NAC on bovine oocyte and embryo development, except for one study, which demonstrated that the addition of 0.6 mM NAC did not significantly improve the quality of bovine oocytes and embryos; the authors inferred that the reason may be because NAC inhibits NF-κB pathway activation during oocyte maturation [28].…”

Section: Introductionmentioning

confidence: 99%

N-acetyl-L-cysteine Improves the Developmental Competence of Bovine Oocytes and Embryos Cultured In Vitro by Attenuating Oxidative Damage and Apoptosis

et al. 2021

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Oxidative stress has been suggested to negatively affect oocyte and embryo quality and developmental competence, resulting in failure to reach full term. In this study, we investigated the effect of N-acetyl-L-cysteine (NAC), a cell-permeating antioxidant, on developmental competence and the quality of oocytes and embryos upon supplementation (0.1–10 mM) in maturation and culture medium in vitro using slaughterhouse-derived oocytes and embryos. The results show that treating oocytes with 1.0 mM NAC for 8 h during in vitro maturation attenuated the intracellular reactive oxygen species (ROS) (p < 0.05) and upregulated intracellular glutathione levels (p < 0.01) in oocytes. Interestingly, we found that NAC affects early embryonic development, not only in a dose-dependent, but also in a stage-specific, manner. Significantly (p < 0.05) decreased cleavage rates (90.25% vs. 81.46%) were observed during the early stage (days 0–2), while significantly (p < 0.05) increased developmental rates (38.20% vs. 44.46%) were observed during the later stage (from day 3) of embryonic development. In particular, NAC supplementation decreased the proportion of apoptotic blastomeres significantly (p < 0.05), resulting in enhanced hatching capability and developmental rates during the in vitro culture of embryos. Taken together, our results suggest that NAC supplementation has beneficial effects on bovine oocytes and embryos through the prevention of apoptosis and the elimination of oxygen free radicals during maturation and culture in vitro.

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N-acetyl-l-cysteine protects porcine oocytes undergoing meiotic resumption from heat stress

Cited by 16 publications

References 53 publications

Nicotinamide mononucleotide supplementation improves the quality of porcine oocytes under heat stress

Nicotinamide mononucleotide supplementation improves the quality of porcine oocytes under heat stress

Idebenone relieves the damage of heat stress on the maturation and developmental competence of porcine oocytes

N-acetyl-L-cysteine Improves the Developmental Competence of Bovine Oocytes and Embryos Cultured In Vitro by Attenuating Oxidative Damage and Apoptosis

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