N-Acetyl-d-Glucosamine Induces Germination in Candida albicans through a Mechanism Sensitive to Inhibitors of cAMP-Dependent Protein Kinase

Castilla, Rocı́o; Passeron, Susana; Cantore, María L.

doi:10.1016/s0898-6568(98)00015-1

Cited by 65 publications

(77 citation statements)

References 45 publications

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“…Stabilization of Ume6 by CO 2 is likely mediated through a Cyr1-independent pathway. GlcNAc, unlike other sugars, stimulates the yeast-to-hypha transition in C. albicans by activating the Cyr1-PKA pathway (Castilla et al 1998;Gunasekera et al 2010) through unknown sensors/receptors. GlcNAc also activates a cAMP-independent pathway to induce the expression of genes needed to catabolize itself (Fig.…”

Section: Environmental Regulation Of Hyphal Morphogenesis Sensing Nutmentioning

confidence: 99%

Fungal Morphogenesis

Lin

Alspaugh

Liu

et al. 2014

Cold Spring Harbor Perspectives in Medicine

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Morphogenesis in fungi is often induced by extracellular factors and executed by fungal genetic factors. Cell surface changes and alterations of the microenvironment often accompany morphogenetic changes in fungi. In this review, we will first discuss the general traits of yeast and hyphal morphotypes and how morphogenesis affects development and adaptation by fungi to their native niches, including host niches. Then we will focus on the molecular machinery responsible for the two most fundamental growth forms, yeast and hyphae. Last, we will describe how fungi incorporate exogenous environmental and host signals together with genetic factors to determine their morphotype and how morphogenesis, in turn, shapes the fungal microenvironment. PROPERTIES OF MORPHOGENESIS IN FUNGAL CELLS

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Section: Environmental Regulation Of Hyphal Morphogenesis Sensing Nutmentioning

confidence: 99%

Fungal Morphogenesis

Lin

Alspaugh

Liu

et al. 2014

Cold Spring Harbor Perspectives in Medicine

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“…Since the increase in intracellular cAMP levels positively affects germ tube formation (Bahn & Sundstrom, 2001;Castilla et al, 1998;Chattaway et al, 1981), degradation of cAMP should diminish or inhibit filamentation. In S. cerevisiae, increasing the expression of BCY1 shifts the equilibrium of association/dissociation of PKA from subunits towards the associated (inactive) state even in the presence of cAMP (Portela et al, 2001), thus blunting downstream effects of PKA on gene activation.…”

Section: Constitutive Expression Of Bcy1 Diminishes Germ Tube Formationmentioning

confidence: 99%

“…tube formation (Bahn & Sundstrom, 2001;Castilla et al, 1998;Chattaway et al, 1981). Signals that increase cAMP levels or addition of exogenous cAMP or dibutyryl cAMP activate the cAMP pathway and promote germ tube formation (Bahn & Sundstrom, 2001;Castilla et al, 1998;Chattaway et al, 1981).…”

Section: Introductionmentioning

confidence: 99%

“…Signals that increase cAMP levels or addition of exogenous cAMP or dibutyryl cAMP activate the cAMP pathway and promote germ tube formation (Bahn & Sundstrom, 2001;Castilla et al, 1998;Chattaway et al, 1981). If, on the other hand, the release of active subunits of PKA is blocked, the cAMP signalling pathway is deactivated, and germ tube induction is suppressed.…”

Section: Introductionmentioning

confidence: 99%

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Integrative, multifunctional plasmids for hypha-specific or constitutive expression of green fluorescent protein in Candida albicans

2003

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The authors have engineered plasmid constructs for developmental and constitutive expression of yeast-enhanced green fluorescent protein (yEGFP3) in Candida albicans. The promoter for the hyphae-specific gene Hyphal Wall Protein 1 (HWP1) conferred developmental expression of yEGFP3 in germ tubes and hyphae but not in yeasts or pseudohyphae when targeted to the ENO1 (enolase) locus in single copy. The pHWP1GFP3 construct allows for the easy visualization of HWP1 promoter activity in individual cells expressing true hyphae without having to prepare RNA for analysis. Constitutive expression of yEGFP was seen in all cell morphologies when the HWP1 promoter was replaced with the ENO1 promoter region. The use of the plasmids for expression of genes other than yEGFP3 was examined by substituting the putative C. albicans BCY1 (SRA1) gene, a component of the cAMP signalling pathway involved in yeast to hyphae transitions, for yEGFP3. Strains overexpressing BCY1 from the ENO1 promoter were inhibited in germ tube formation and filamentation in both liquid and solid media, a phenotype consistent with keeping protein kinase A in its inactive form by association with Bcy1p. The plasmids are suitable for studies of germ tube induction or assessing germ tube formation by measuring yEGFP3 expression, for inducible expression of genes concomitant with germ tube formation by the HWP1 promoter, for constitutive expression of genes by the ENO1 promoter, and for expressing yEGFP3 using a promoter of choice.

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“…dbcAMP is known to promote hyphal growth in C. albicans via the cAMP-PKA pathway [10], and it is also reported that GlcNAc activates the cAMP-PKA pathway and promotes hyphal growth in C. albicans [11,12]; therefore, the effect of dbcAMP and GlcNAc on hyphal growth inhibition by valinomycin was examined ( Table 1). Independent of the addition of dbcAMP and GlcNAc, hyphal growth was inhibited by 1.0 mg/ml of valinomycin.…”

Section: Analysis Of Hyphal Growth Inhibition By Valinomycin Using Dbmentioning

confidence: 99%

Relationship between Cell Morphology and Intracellular Potassium Concentration in Candida albicans

et al. 2006

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Previously we reported that valinomycin inhibited hyphal growth and induced growth as a chain of yeast cells under hyphal growth induction conditions in Candida albicans. To elucidate the hyphal growth inhibition by valinomycin, we examined the effect of various chemicals on the morphology and found that miconazole inhibited hyphal growth as well as valinomycin: both compounds promoted the leakage of potassium from cells. Analysis of intracellular potassium suggested that hyphal cells contain potassium at high concentrations in comparison with yeast cells. Hyphal growth inhibition by valinomycin was obstructed by the addition of serum. Potassium measurement showed that the addition of serum causes an increase in intracellular potassium, suggesting that the obstruction by serum might be due to an increase in intracellular potassium. The above-mentioned results strongly suggest that the addition of valinomycin and miconazole decreased interacellular potassium and this decrease inhibited hyphal transition.

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N-Acetyl-d-Glucosamine Induces Germination in Candida albicans through a Mechanism Sensitive to Inhibitors of cAMP-Dependent Protein Kinase

Cited by 65 publications

References 45 publications

Fungal Morphogenesis

Fungal Morphogenesis

Integrative, multifunctional plasmids for hypha-specific or constitutive expression of green fluorescent protein in Candida albicans

Relationship between Cell Morphology and Intracellular Potassium Concentration in Candida albicans

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