N‐(3‐Chlor‐2‐hydroxypropyl)‐Valin als Hämoglobinaddukt des Epichlorhydrins [Biomonitoring Methods in German language, 2012]

Bader, Michael; Rosenberger, W.; Tsikas, Dimitrios; Gutzki, Frank‐Mathias

doi:10.1002/3527600418.bi10689d0020

Cited by 2 publications

(1 citation statement)

References 11 publications

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“…Von der Arbeitsgruppe "Analysen in biologischem Material" wurden die Verfahren zur Bestimmung der S-(3-Chlor-2-hydroxypropyl)-merkaptursäure (CHPMA) (Eckert et al 2015) sowie der Addukte N-(3-Chlor-2-hydroxypropyl)-valin (CHPV) (Bader et al 2012) und N-(2,3-Dihydroxypropyl)-valin (DHPV) (Müller et al 2012) in die Methodensammlung der Kommission aufgenommen.…”

Section: Untersuchungsmethodenunclassified

1‐Chlor‐2,3‐epoxypropan (Epichlorhydrin) [BAT Value Documentation in German language, 2017]

Göen

Bader

Drexler

et al. 2017

The MAK‐Collection for Occupational Health and Safety

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The German Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area has evaluated EKA (exposure equivalents for carcinogenic substances) for epichlorohydrin (CAS‐No 106‐89‐8) in 2017. Available publications are described in detail. In rodents, epichlorohydrin induces malignant lymphomas, hyperplasias, forestomach papillomas and carcinomas, subcutaneous fibromas and lung and pituitary tumours. Epichlorohydrin was classified in category 2 for carcinogenic substances. The substance can easily pass through the skin, so biological monitoring is indicated for a valid individual risk assessment. In several biomonitoring studies the mercapturic acids derivatives N‐acetyl‐S‐(3‐chloro‐2‐hydroxypropyl)‐L‐cysteine (CHPMA) and N‐acetyl‐S‐(2,3‐dihydroxypropyl)‐L‐cysteine (DHPMA) in urine as well as the hemoglobin adducts N‐(3‐chloro‐2‐hydroxypropyl)valine and N‐(2,3‐dihydroxypropyl)valine are suggested as biomarkers after epichlorohydrin exposure. Validated analytical procedures for their quantification are available. Data of a correlation of epichlorohydrin in the air and the CHPMA‐excretion in urine were considered for the evaluation of exposure equivalents for carcinogenic substances. Sampling time is at the end of exposure or end of shift and after long term exposure at the end of the shift after several shifts.

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Section: Untersuchungsmethodenunclassified

1‐Chlor‐2,3‐epoxypropan (Epichlorhydrin) [BAT Value Documentation in German language, 2017]

Göen

Bader

Drexler

et al. 2017

The MAK‐Collection for Occupational Health and Safety

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Merkaptursäuren des 2‐Chloroprens, des Epichlorhydrins und des 1,3‐Butadiens im Urin [Biomonitoring Methods in German Language, 2015]

Eckert¹,

Göen

Hartwig

2016

The MAK‐Collection for Occupational Health and Safety

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The analytical method permits simultaneous determination of the mercapturic acid of epichlorohydrin i.e. N‐acetyl‐S‐(3‐chloro‐2‐hydroxypropyl) cysteine (CHPMA) and of four mercapturic acids of 2‐chloroprene in urine: N‐acetyl‐S‐(4‐chloro‐3‐oxobutyl) cysteine (Cl‐MA I), N‐acetyl‐S‐(3‐chloro‐2‐hydroxy‐3‐butenyl) cysteine (Cl‐MA III), N‐acetyl‐S‐(4‐hydroxy‐3‐oxobutyl) cysteine (HOBMA) and N‐acetyl‐S‐(3,4‐dihydroxybutyl) cysteine (DHBMA). The mercapturic acids HOBMA and DHBMA are also formed after exposure to 1,3‐butadiene. For determination, the urine samples are buffered to pH 2.5, spiked with isotopelabeled internal standards (d 3 ‐CHPMA, d 3 ‐Cl‐MA I, d 3 ‐Cl‐MA III, d 3 ‐HOBMA and d 7 ‐DHBMA), processed using online solid phase extraction (online SPE) and LC–MS/MS. For that purpose the analytes are enriched on a C18 pre‐concentration column [RAM (Restricted Access Material) phase]. Subsequently, the analytes are transferred in backflush mode from the RAM phase to the analytical column, separated chromatographically and finally subjected to tandem mass spectrometric detection. Calibration is carried out using standard solutions in pooled urine which are processed and analyzed in the same way as the samples to be analyzed.

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N‐(3‐Chlor‐2‐hydroxypropyl)‐Valin als Hämoglobinaddukt des Epichlorhydrins [Biomonitoring Methods in German language, 2012]

Cited by 2 publications

References 11 publications

1‐Chlor‐2,3‐epoxypropan (Epichlorhydrin) [BAT Value Documentation in German language, 2017]

1‐Chlor‐2,3‐epoxypropan (Epichlorhydrin) [BAT Value Documentation in German language, 2017]

Merkaptursäuren des 2‐Chloroprens, des Epichlorhydrins und des 1,3‐Butadiens im Urin [Biomonitoring Methods in German Language, 2015]

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