Myricetin relieves LPS‐induced mastitis by inhibiting inflammatory response and repairing the blood–milk barrier

Kan, Xingchi; Liu, Bingrun; Guo, Wenjin; Wei, Libin; Lin, Yuanqing; Guo, Yingcheng; Gong, Qian; Li, Yanwei; Xu, Dianwen; Cao, Yu; Huang, Bingxu; Dong, Aiwen; Ma, He; Fu, Shoupeng; Liu, Juxiong

doi:10.1002/jcp.28288

Cited by 63 publications

(50 citation statements)

References 37 publications

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“…Similarly, study showed that adding LAB prevented E. coli infection and depressed the expression of proinflammation cytokine IL-8 and IL-1β of endometrial cells in vitro (Genis et al 2016). MPO activity is a quantitative assessment of neutrophil infiltration into mammary gland (Kan et al 2019), and our result showed that milk activity of MPO was significantly decreased by supplementation of yeast, LAB or the mixture. In general, combination of yeast and LAB had potential at regulating inflammation of bovine mammary gland.…”

Section: Discussionsupporting

confidence: 62%

Impact of yeast and lactic acid bacteria on mastitis and milk microbiota composition of dairy cows

et al. 2020

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This experiment was conducted to evaluate the impact of yeast and lactic acid bacteria (LAB) on mastitis and milk microbiota composition of dairy cows. Thirty lactating Holstein cows with similar parity, days in milk were randomly assigned to five treatments, including: (1) Health cows with milk SCC < 500,000 cells/mL, no clinical signs of mastitis were found, fed basal total mixed ration (TMR) without supplementation (H); (2) Mastitis cows with milk SCC > 500,000 cells/mL, fed basal TMR without supplementation (M); (3) Mastitis cows fed basal TMR supplemented with 8 g day −1 yeast (M + Y); (4) Mastitis cows fed basal TMR supplemented with 8 g day −1 LAB (M + L); (5) Mastitis cows (milk SCC > 500,000 cells/mL) fed basal TMR supplemented with 4 g day −1 yeast and 4 g day −1 LAB (M + Y + L). Blood and milk sample were collected at day 0, day 20 and day 40. The results showed efficacy of probiotic: On day 20 and day 40, milk SCC in H, M + Y, M + L, M + Y + L was significantly lower than that of M (P < 0.05). Milk concentration of TNF-α, IL-6 and IL-1β in M + Y + L were significantly reduced compared with that of M on day 40 (P < 0.05). Milk Myeloperoxidase (MPO) and N-Acetyl-β-d-Glucosaminidase (NAG) activity of M + Y, M + L, M + L + Y were lower than that of M on day 40 (P < 0.05). At genus level, Staphylococcus, Chryseobacterium and Lactococcus were dominant. Supplementation of LAB decreased abundance of Enterococcus and Streptococcus, identified as mastitis-causing pathogen. The results suggested the potential of LAB to prevent mastitis by relieving mammary gland inflammation and regulating milk microorganisms.

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Section: Discussionsupporting

confidence: 62%

Impact of yeast and lactic acid bacteria on mastitis and milk microbiota composition of dairy cows

et al. 2020

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“…In vitro, the application of LPS and ATP is to stimulate mMECs and to construct an in vitro mastitis model. Based on existent reports, two main interdependent processes will aggravate the mastitis inflammation: one is recruitment of inflammatory cells and the other is upregulation of proinflammatory cytokines [ 37 ]. In vivo, these experiments demonstrated that the dioscin reduces the mastitis symptoms induced by LPS in mice.…”

Section: Discussionmentioning

confidence: 99%

Dioscin Improves Pyroptosis in LPS-Induced Mice Mastitis by Activating AMPK/Nrf2 and Inhibiting the NF-κB Signaling Pathway

Ran

Zhang

Yang

et al. 2020

Oxidative Medicine and Cellular Longevity

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Dioscin, a natural steroid saponin, has been shown to have anti-inflammatory effects, but its protective mechanism against mastitis is still unknown. NLRP3 inflammasome and pyroptosis play important roles in the pathogenesis of many inflammatory diseases, including mastitis. The purpose of this study was to explore the effect of dioscin on lipopolysaccharide- (LPS-) induced mastitis in vivo and in vitro and its mechanism of action. In vivo experiments, dioscin can reduce the inflammatory lesions and neutrophil motility in mammary tissue. Moreover, dioscin also can reduce the production of proinflammatory factors such as interleukin-1 beta (IL-1β) and inhibit the activation of NLRP3 inflammasome in LPS-induced mice mastitis. In vitro experiments, the results showed that dioscin inhibited the inflammatory response and the activation of NLRP3 inflammasome, but the survival rate of mouse mammary epithelial cells (mMECs) induced by LPS+ATP is increased. Subsequently, the experiment convinces that dioscin can reduce LPS+ATP-induced mMEC pyroptosis by adding Ac-DEVD-CHO (a caspase-3 inhibitor). Further mechanistic studies demonstrate that dioscin can activate AMPK/Nrf2 to inhibit NLRP3/GSDMD-induced mMEC pyroptosis. In summary, this paper reveals a novel function of dioscin on mMEC pyroptosis and provides a new potential therapy of dioscin for the treatment and prevention of mastitis.

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“…The incidence of mastitis is high during postpartum lactation, which results in reduced breast feeding in humans and milk production in dairy animals [ 1 , 2 ]. Studies have shown that inflammation in multiple organs was associated with oxidative stress [ 5 ], but it has not been explored clearly in the mammary gland.…”

Section: Discussionmentioning

confidence: 99%

“…Milk is important for the growth and development of the youth and nutrition of humans. Unfortunately, the incidence of mastitis arises during lactation, which causes severe pain and even sepsis and hinders breast feeding in humans and reduces milk production in dairy cows [ 1 , 2 ]. Gram-negative bacteria Escherichia coli is one of the main pathogens responsible for mastitis, and lipopolysaccharide (LPS) is a major component of the outer cell membranes of gram-negative bacteria.…”

Section: Introductionmentioning

confidence: 99%

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Nrf2‐ARE Signaling Partially Attenuates Lipopolysaccharide‐Induced Mammary Lesions via Regulation of Oxidative and Organelle Stresses but Not Inflammatory Response in Mice

Shao

Hou

et al. 2021

Oxidative Medicine and Cellular Longevity

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The incidence of mastitis is high during the postpartum stage, which causes severe pain and hinders breast feeding in humans and reduces milk production in dairy cows. Studies suggested that inflammation in multiple organs is associated with oxidative stress and nuclear factor E2-related factor 2 (Nrf2)-antioxidant response element pathway is one of the most important antioxidant pathways, but the effects of Nrf2 on antioxidation in the mammary gland during mastitis are still unclear. In this study, intramammary lipopolysaccharide (LPS) challenge was carried out in wild-type (WT) and Nrf2 knockout mice. Results showed that the expression of Nrf2 affected the expression of milk protein genes (Csn2 and Csn3). Importantly, LPS treatment increased the expression of Nrf2 and HO-1 and the content of glutathione in the mammary gland of WT mice, but not in Nrf2(-/-) mice. The expression levels of glutathione synthesis genes (GCLC, GCLM, and xCT) were lower in Nrf2(-/-) mice than in WT mice. Moreover, mitochondrial-dependent apoptotic and endoplasmic reticulum stress were significantly relieved in WT mice compared with that in Nrf2(-/-) mice. In summary, the expression of Nrf2 may play an important role in prevention of oxidative and organelle stresses during endotoxin-induced mastitis in mouse mammary gland.

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Myricetin relieves LPS‐induced mastitis by inhibiting inflammatory response and repairing the blood–milk barrier

Cited by 63 publications

References 37 publications

Impact of yeast and lactic acid bacteria on mastitis and milk microbiota composition of dairy cows

Impact of yeast and lactic acid bacteria on mastitis and milk microbiota composition of dairy cows

Dioscin Improves Pyroptosis in LPS-Induced Mice Mastitis by Activating AMPK/Nrf2 and Inhibiting the NF-κB Signaling Pathway

Nrf2‐ARE Signaling Partially Attenuates Lipopolysaccharide‐Induced Mammary Lesions via Regulation of Oxidative and Organelle Stresses but Not Inflammatory Response in Mice

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