MyoVision: software for automated high-content analysis of skeletal muscle immunohistochemistry

Wen, Yan; Murach, Kevin A.; Vechetti, Ivan J.; Fry, Christopher S.; Vickery, Chase Daniel; Peterson, Charlotte A.; McCarthy, John J.; Campbell, Kenneth S.

doi:10.1152/japplphysiol.00762.2017

Cited by 182 publications

(181 citation statements)

References 34 publications

(47 reference statements)

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“…Three 20× magnification images per muscle were analyzed for myofiber cross-sectional area (~200 fibers) using automated MyoVision software (University of Kentucky) as described. (40) The investigators were blinded to treatment of subjects.…”

Section: Histologymentioning

confidence: 99%

Zoledronic Acid Improves Muscle Function in Healthy Mice Treated with Chemotherapy

Hain

Jude

et al. 2019

Journal of Bone and Mineral Research

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Carboplatin is a chemotherapy drug used to treat solid tumors but also causes bone loss and muscle atrophy and weakness. Bone loss contributes to muscle weakness through bone-muscle crosstalk, which is prevented with the bisphosphonate zoledronic acid (ZA). We treated mice with carboplatin in the presence or absence of ZA to assess the impact of bone resorption on muscle. Carboplatin caused loss of body weight, muscle mass, and bone mass, and also led to muscle weakness as early as 7 days after treatment. Mice treated with carboplatin and ZA lost body weight and muscle mass but did not lose bone mass. In addition, muscle function in mice treated with ZA was similar to control animals. We also used the anti-TGFβ antibody (1D11) to prevent carboplatin-induced bone loss and showed similar results to ZA-treated mice. We found that atrogin-1 mRNA expression was increased in muscle from mice treated with carboplatin, which explained muscle atrophy. In mice treated with carboplatin for 1 or 3 days, we did not observe any bone or muscle loss, or muscle weakness. In addition, reduced caloric intake in the carboplatin treated mice did not cause loss of bone or muscle mass, or muscle weakness. Our results show that blocking carboplatin-induced bone resorption is sufficient to prevent skeletal muscle weakness and suggests another benefit to bone therapy beyond bone in patients receiving chemotherapy.n 368 HAIN ET AL. TissueMice were euthanized and the tibialis anterior (TA), soleus, and gastrocnemius were dissected. The extensor digitorum longus (EDL) muscle was dissected for muscle contractility. Muscles were either snap frozen in liquid nitrogen for biochemical analysis or embedded in optimum cutting temperature (O.C.T., Tissue Tek, Torrance, CA, USA) compound in cryomolds and frozen in liquid nitrogencooled 2-methylbutane (isopentane) for histological analysis. (38) Journal of Bone and Mineral Research ZA IMPROVES MUSCLE FUNCTION IN HEALTHY MICE TREATED WITH CHEMOTHERAPY 369 n 40. Wen Y, Murach KA, Vechetti IJ Jr, et al. MyoVision: software for automated high-content analysis of skeletal muscle immunohistochemistry. J Appl Physiol. 2018;124(1):40-51. 41. Edwards JR, Nyman JS, Lwin ST, et al. Inhibition of TGF-beta signaling by 1D11 antibody treatment increases bone mass and quality in vivo.

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Section: Histologymentioning

confidence: 99%

Zoledronic Acid Improves Muscle Function in Healthy Mice Treated with Chemotherapy

Hain

Jude

et al. 2019

Journal of Bone and Mineral Research

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“…Histological analysis of muscle sections has been a staple of muscle physiology and neuromuscular disease research for decades; indeed, a number of neuromuscular diseases were originally named for their distinct histopathological features [25][26][27][28]. Myovision, SMASH, and MuscleJ [16,18,19]. Of these, MuscleJ has by far the most complete collection of features, with a number of capabilities that are absent even in Myosoft.…”

Section: Discussionmentioning

confidence: 99%

“…However, despite the value of such analysis, it is often performed manually, which is both labor-intensive and time-consuming. To offset this obstacle, several groups have developed software that automates analysis of muscle histology [16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Myosoft: an automated muscle histology analysis tool using machine learning algorithm utilizing FIJI/ImageJ software

Encarnacion-Rivera

Foltz

Hartzell

et al. 2019

Preprint

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Background:Skeletal muscle is comprised of a heterogeneous population of muscle fibers which can be classified by their metabolic and contractile properties (fiber "types"). Fiber type is a primary determinant of muscle function along with fiber size (cross-sectional area). The fiber type composition of a muscle responds to physiological changes like exercise and aging and is often altered in disease states. Thus, analysis of fiber size and type in histological muscle preparations is a useful method for quantifying key indicators of muscle function and for measuring responses to a variety of stimuli or stressors. These analyses are nearubiquitous in the fields of muscle physiology and myopathy, but are most commonly performed manually, which is highly labor-and time-intensive. To offset this obstacle, we developed Myosoft, a novel method to automate morphometric and fiber type analysis in muscle sections stained with fluorescent antibodies. Methods:Muscle sections were stained for cell membrane (laminin) and myofiber type (myosin heavy chain isoforms).Myosoft, running in the open access software platform FIJI (ImageJ), was used to analyze myofiber size and type in transverse sections of entire gastrocnemius/soleus muscles. Results:Myosoft provides accurate analysis of muscle histology >50-times faster than manual analysis. We demonstrate that Myosoft is capable of handling high-content images even when image or staining quality is suboptimal, which is a marked improvement over currently available, comparable programs. Conclusions:Myosoft is a reliable, accurate, high-throughput, and convenient tool to analyze high-content muscle histology. Myosoft is freely available to download from Github at https://github.com/Hyojung-Choo/Myosoft/tree/Myosoft-hub.

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“…Slides were cover slipped using ProLong Gold Antifade reagent (Life Technologies). Image analysis was performed using Myovision software (65). Skeletal muscle fiber size was analyzed by measuring ≥ 350 transfected muscle fibers per muscle, per animal (10x magnification).…”

Section: Histologymentioning

confidence: 99%

Identification and Characterization of Fbxl22, a novel skeletal muscle atrophy-promoting E3 ubiquitin ligase

Hughes

Baehr

Driscoll

et al. 2020

Preprint

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Muscle-specific E3 ubiquitin ligases have been identified in muscle atrophy-inducing conditions. The purpose of the current study was to explore the functional role of Fbxl22, and a newly identified splice variant , in skeletal muscle homeostasis and neurogenic muscle atrophy. The full-length gene and novel splice variant are transcriptionally induced early (after 3 days) during neurogenic muscle atrophy. In vivo overexpression of Fbxl22 isoforms in mouse skeletal muscle lead to evidence of myopathy/atrophy suggesting that both are involved in the process of neurogenic muscle atrophy. Knockdown of Fbxl22 in MuRF1 KO muscles resulted in significant additive muscle sparing at 7 days of denervation. Targeting two E3 ubiquitin ligases appears to have a strong additive effect on protecting muscle mass loss with denervation and these findings have important implications in the development of therapeutic strategies to treat muscle atrophy.

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MyoVision: software for automated high-content analysis of skeletal muscle immunohistochemistry

Cited by 182 publications

References 34 publications

Zoledronic Acid Improves Muscle Function in Healthy Mice Treated with Chemotherapy

Zoledronic Acid Improves Muscle Function in Healthy Mice Treated with Chemotherapy

Myosoft: an automated muscle histology analysis tool using machine learning algorithm utilizing FIJI/ImageJ software

Identification and Characterization of Fbxl22, a novel skeletal muscle atrophy-promoting E3 ubiquitin ligase

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