Myostatin in relation to physical activity and dysglycaemia and its effect on energy metabolism in human skeletal muscle cells

Hjorth, Marit; Pourteymour, Shirin; Görgens, Sven W.; Langleite, Torgrim M.; Lee, Sindre; Holen, Torgeir; Gulseth, Hanne Løvdal; Birkeland, Kåre I.; Jensen, Jørgen; Drevon, Christian A.; Norheim, Frode

doi:10.1111/apha.12631

Cited by 65 publications

(66 citation statements)

References 88 publications

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“…One of these transcripts encodes MSTN (FC = −1.7), which is a negative regulator of muscle growth. This result was recently reported and discussed in another publication [33].…”

Section: Resultssupporting

confidence: 81%

Global mRNA sequencing of human skeletal muscle: Search for novel exercise-regulated myokines

Pourteymour

Eckardt

Holen

et al. 2017

Molecular Metabolism

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ObjectiveSkeletal muscle is an important secretory organ, producing and releasing numerous myokines, which may be involved in mediating beneficial health effects of physical activity. More than 100 myokines have been identified by different proteomics approaches, but these techniques may not detect all myokines. We used mRNA sequencing as an untargeted approach to study gene expression of secreted proteins in skeletal muscle upon acute as well as long-term exercise.MethodsTwenty-six middle-aged, sedentary men underwent combined endurance and strength training for 12 weeks. Skeletal muscle biopsies from m. vastus lateralis and blood samples were taken before and after an acute bicycle test, performed at baseline as well as after 12 weeks of training intervention. We identified transcripts encoding secretory proteins that were changed more than 1.5-fold in muscle after exercise. Secretory proteins were defined based on either curated UniProt annotations or predictions made by multiple bioinformatics methods.ResultsThis approach led to the identification of 161 candidate secretory transcripts that were up-regulated after acute exercise and 99 that where increased after 12 weeks exercise training. Furthermore, 92 secretory transcripts were decreased after acute and/or long-term physical activity. From these responsive transcripts, we selected 17 candidate myokines sensitive to short- and/or long-term exercise that have not been described as myokines before. The expression of these transcripts was confirmed in primary human skeletal muscle cells during in vitro differentiation and electrical pulse stimulation (EPS). One of the candidates we identified was macrophage colony-stimulating factor-1 (CSF1), which influences macrophage homeostasis. CSF1 mRNA increased in skeletal muscle after acute and long-term exercise, which was accompanied by a rise in circulating CSF1 protein. In cultured muscle cells, EPS promoted a significant increase in the expression and secretion of CSF1.ConclusionWe identified 17 new, exercise-responsive transcripts encoding secretory proteins. We further identified CSF1 as a novel myokine, which is secreted from cultured muscle cells and up-regulated in muscle and plasma after acute exercise.

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“…One of these transcripts encodes MSTN (FC = −1.7), which is a negative regulator of muscle growth. This result was recently reported and discussed in another publication [33].…”

Section: Resultssupporting

confidence: 81%

Global mRNA sequencing of human skeletal muscle: Search for novel exercise-regulated myokines

Pourteymour

Eckardt

Holen

et al. 2017

Molecular Metabolism

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“…The balance between protein synthesis and degradation in the skeletal muscle is regulated by several molecules such as myostatin, cytokines, vitamin D, and irisin [8–12]. Myostatin is a transforming growth factor beta (TGF-β) that inhibits muscle cell growth by modulating Akt signaling pathways [5].…”

Section: Introductionmentioning

confidence: 99%

Serum irisin and myostatin levels after 2 weeks of high-altitude climbing

Śliwicka¹,

Cisoń

Kasprzak³

et al. 2017

PLoS ONE

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Exposure to high-altitude hypoxia causes physiological and metabolic adaptive changes by disturbing homeostasis. Hypoxia-related changes in skeletal muscle affect the closely interconnected energy and regeneration processes. The balance between protein synthesis and degradation in the skeletal muscle is regulated by several molecules such as myostatin, cytokines, vitamin D, and irisin. This study investigates changes in irisin and myostatin levels in male climbers after a 2-week high-altitude expedition, and their association with 25(OH)D and indices of inflammatory processes. The study was performed in 8 men aged between 23 and 31 years, who participated in a 2-week climbing expedition in the Alps. The measurements of body composition and serum concentrations of irisin, myostatin, 25(OH)D, interleukin-6, myoglobin, high-sensitivity C-reactive protein, osteoprotegerin, and high-sensitivity soluble receptor activator of NF-κB ligand (sRANKL) were performed before and after expedition. A 2-week exposure to hypobaric hypoxia caused significant decrease in body mass, body mass index (BMI), free fat mass and irisin, 25-Hydroxyvitamin D levels. On the other hand, significant increase in the levels of myoglobin, high-sensitivity C-reactive protein, interleukin-6, and osteoprotegerin were noted. The observed correlations of irisin with 25(OH)D levels, as well as myostatin levels with inflammatory markers and the OPG/RANKL ratio indicate that these myokines may be involved in the energy-related processes and skeletal muscle regeneration in response to 2-week exposure to hypobaric hypoxia.

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“…To compensate for this obvious bias, the appearance date will be considered in the evaluation process. Our best performing articles in 2016 are currently . The latter two are from the same group!…”

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confidence: 97%

Acta Physiologica Award: outperforming original articles from 2015 to 2017

Persson

2017

Acta Physiologica

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Myostatin in relation to physical activity and dysglycaemia and its effect on energy metabolism in human skeletal muscle cells

Cited by 65 publications

References 88 publications

Global mRNA sequencing of human skeletal muscle: Search for novel exercise-regulated myokines

Global mRNA sequencing of human skeletal muscle: Search for novel exercise-regulated myokines

Serum irisin and myostatin levels after 2 weeks of high-altitude climbing

Acta Physiologica Award: outperforming original articles from 2015 to 2017

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