Myosin VI contributes to maintaining epithelial barrier function

Liao, Yuwei; Wu, Xiaojia; Jia, Jia; Wu, Xiao‐Lei; Hong, Tao; Ling-xin, Meng; Wu, Xian

doi:10.1186/1423-0127-20-68

Cited by 6 publications

(4 citation statements)

References 21 publications

(28 reference statements)

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“…Cumulative evidence shows incompetent of epithelial cells in the degradation of the endocytic proteins under given circumstances (Huang et al, ; Li et al, ; Liao et al, ) that results in epithelial barrier dysfunction. We considered whether additional avb6 facilitates the degradation of the endocytic antigens under circumstances such as in high levels of proinflammatory cytokines.…”

Section: Resultsmentioning

confidence: 99%

Alphavbeta6 is required in maintaining the intestinal epithelial barrier function

Chen

et al. 2014

Cell Biology International

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Epithelial barrier dysfunction is involved in a large number of diseases, but the pathogenesis is unclear. Integrin alphavbeta6 (avb6) in involved in the maintenance of the mucosal homeostasis. We have investigated the role of avb6 in maintaining the epithelial barrier function. Using T84 monolayers cultures, transepithelial electric resistance (TER) and permeability to ovalbumin (OVA) were measured as indicators of functioning. The antigenicity of OVA collected from the Transwell basal chambers was assessed using OVA-specific T cell proliferation. Knockdown of the avb6 genes increased the permeability of T84 monolayers to OVA, but did not affect TER. The deficiency of avb6-related hyperpermeability in T84 monolayers could be compensated by adding exogenous avb6 to the culture. The OVA samples collected from the basal chambers had strong antigenicity as it markedly induced the antigen specific T cell proliferation. Addition of recombinant avb6 blocked increases in permeability of T84 monolayers to OVA induced by tumor necrosis factor-α.

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Section: Resultsmentioning

confidence: 99%

Alphavbeta6 is required in maintaining the intestinal epithelial barrier function

Chen

et al. 2014

Cell Biology International

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“…Myosins, a group of ATP-dependent motor proteins, are reported to be responsible for actin-based motility, trafficking, intracellular transport, and possible regulation of signal transduction in non-muscle cells (Wu et al, 2000;Berg et al, 2001;Dunn et al, 2006). MYO6 was shown to function in iris development, maintaining epithelial barrier function and mammalian auditory systems (Liao et al, 2013;Samuels et al, 2013;Williams et al, 2013). Besides, MYO6 has also been shown to play a critical role in cancer cell migration including ovary cancers and prostate cancers (Yoshida et al, 2004;Dunn et al, 2006;Wei et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Overexpression of myosin VI regulates gastric cancer cell progression

Wang

Mao

et al. 2016

Gene

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Myosin VI (MYO6) is a unique member of the myosin superfamily. Although it has been reported to participate in human cancer progression, the role of MYO6 in gastric cancer remains unclear. In this study, we found the expression of MYO6 gene was higher in gastric cancer tissues than in the normal tissues by Oncomine database mining and affects patient overall survival using the Kaplan-Meier plotter online analysis. Additionally, the expression levels of MYO6 were widely expressed in gastric cancer cells by quantitative real-time Polymerase Chain Reaction (qRT-PCR) and western blot assay. Then knockdown of MYO6 significantly suppressed the proliferation and colony formation abilities of AGS and MGC80-3 cells. Moreover, cell cycle analysis showed that inhibition of MYO6 induced cell cycle arrested in G0/G1 phase in AGS and MGC80-3 cells. Further analysis showed knockdown of MYO6 downregulated cell-cycle activators cyclin A and cyclin D1 and upregulated cell-cycle inhibitor p21, as determined by qRT-PCR and western blot analysis in MGC80-3 cells. Meanwhile, MYO6 inhibition significantly induced apoptosis in AGS and MGC80-3 cells. Also, knockdown of MYO6 increased the expression of apoptosis-related proteins Bax and cleaved Caspase-3, and decreased Bcl-2 expression by western blot analysis in MGC80-3 cells. In addition, MYO6 knockdown also inhibited cell migration ability in MGC80-3 cells. Taken together, our study indicates that MYO6 may play an important role in gastric cancer tumorigenesis and may serve as a potential therapeutic target in human gastric cancer.

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“…It is possible to infer that genes related to protein structure and cell proliferation may be involved with the inflammatory phase, adding to extracellular matrix remodeling, new blood vessels formation and epithelia (Theilgaard-Mönch et al, 2004). In fact, the MYO6 gene, likewise related to cytoskeleton rebuilding (actin-myosin) in inflammatory conditions (Liao et al, 2013), is another significant gene associated within the MeSH term Myosin Type I. Thus, MYO6 may play a critical role in epithelial barrier function.…”

Section: Chromosome Regions Affecting Tick Resistance and Overrepresentation Analysismentioning

confidence: 99%

Analyses of reaction norms reveal new chromosome regions associated with tick resistance in cattle

Mota

Silva

Lopes

et al. 2018

Animal

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Despite single nucleotide polymorphism (SNP) availability and frequent cost reduction has allowed genome-wide association studies even in complex traits as tick resistance, the use of this information source in SNP by environment interaction context is unknown for many economically important traits in cattle. We aimed at identifying putative genomic regions explaining differences in tick resistance in Hereford and Braford cattle under SNP by environment point of view as well as to identify candidate genes derived from outliers/significant markers. The environment was defined as contemporary group means of tick counts, since they seemed to be the most appropriate entities to describe the environmental gradient in beef cattle. A total of 4363 animals having tick counts (n=10 673) originated from 197 sires and 3966 dams were used. Genotypes were acquired on 3591 of these cattle. From top 1% SNPs (410) having the greatest effects in each environment, 75 were consistently relevant in all environments, which indicated SNP by environment interaction. The outliers/significant SNPs were mapped on chromosomes 1, 2, 5, 6, 7, 9, 11, 13, 14, 15, 16, 18, 21, 23, 24, 26 and 28, and potential candidate genes were detected across environments. The presence of SNP by environment interaction for tick resistance indicates that genetic expression of resistance depends upon tick burden. Markers with major portion of genetic variance explained across environments appeared to be close to genes with different direct or indirect functions related to immune system, inflammatory process and mechanisms of tissue destruction/repair, such as energy metabolism and cell differentiation.

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Myosin VI contributes to maintaining epithelial barrier function

Cited by 6 publications

References 21 publications

Alphavbeta6 is required in maintaining the intestinal epithelial barrier function

Alphavbeta6 is required in maintaining the intestinal epithelial barrier function

Overexpression of myosin VI regulates gastric cancer cell progression

Analyses of reaction norms reveal new chromosome regions associated with tick resistance in cattle

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