Myosin VI and VIIa distribution among inner ear epithelia in diverse fishes

Coffin, Allison B.; Dabdoub, Alain; Kelley, Matthew W.; Popper, Arthur N.

doi:10.1016/j.heares.2006.11.004

Cited by 23 publications

(11 citation statements)

References 68 publications

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“…We also assessed regeneration with the antibody-based HC markers myosin-VI (Hasson and Mooseker, 1994;Hasson et al, 1997;Coffin et al, 2007) and HCS-1 (Gale et al, 2000;LopezSchier and Hudspeth, 2006). Hair cells were concurrently immunostained and counted for both markers.…”

Section: Lateral Line Hair Cell Regeneration After Neomycin-induced Dmentioning

confidence: 99%

Notch Signaling Regulates the Extent of Hair Cell Regeneration in the Zebrafish Lateral Line

Y.¹,

Rubel²,

Raible³

2008

J. Neurosci.

219

320

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Section: Lateral Line Hair Cell Regeneration After Neomycin-induced Dmentioning

confidence: 99%

Notch Signaling Regulates the Extent of Hair Cell Regeneration in the Zebrafish Lateral Line

Y.¹,

Rubel²,

Raible³

2008

J. Neurosci.

219

320

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“…5B). The blot was probed with a rabbit polyclonal antibody for MYO6 that had been previously characterised [7,15,16,17]. A ~140-kDa band of MYO6 was abundant in tissues from wild-type mice but was not detectable in those of the rsv mutant.…”

Section: Expression and Mutation Analyses Of The Myosin VI Gene And Pmentioning

confidence: 99%

Phenotypic and Expression Analysis of a Novel Spontaneous Myosin VI Null Mutant Mouse

et al. 2010

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Abstract:In humans, hearing is a major factor in quality of life. Mouse models are important tools for the discovery of genes responsible for genetic hearing loss, often enabling analysis of the processes that regulate the onset of deafness in humans. Thus far, at least 400 deafness mutants have been discovered in laboratory mouse populations and used in the study of deafness. Here we report the discovery of a new spontaneous recessive Rinshoken shaker/waltzer (rsv) mutant derived from our in-house C57BL/6J stock, which exhibits circling and/or head-tossing behaviour and complete lack of auditory brain response to any sound pressure. The hearing and balance phenotypes are associated with structural defects, in particular, disorganisation and fusion of stereocilia in the inner ear hair cells. Two sets of intersubspecific N 2 mice were generated for the positional cloning of the rsv mutation. The mutant locus was mapped to a 4.8-Mb region of chromosome 9, which contains myosin VI (Myo6), a gene responsible for deafness in humans and Snell's waltzer mutation in mice. The rsv mutant showed reduced expressions of Myo6 mRNA and MYO6 protein in the inner ear. Moreover, no immunoreactivity was observed in the cochlear and vestibular hair cells in the rsv mutant mice. We sequenced the genomic region (30,154 bp) of Myo6, including all coding exons, a non-coding exon, UTRs and the Myo6 promoter; however, no mutation was discovered in these regions. We therefore speculate that loss of MYO6 expression might cause shaker/waltzer behaviour and deafness in the rsv mutant; also, loss of MYO6 expression might be the result of mutations in an unidentified regulatory region(s) of the gene.

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“…There has been no systematic characterization of unconventional myosins in zebrafish. Mutations generating defects in sensory neuron development and function have led to the cloning of members of the Myo6 and Myo7 families (Ernest et al, 2000;Kappler et al, 2004;Seiler et al, 2004;Coffin et al, 2007). Since the zebrafish embryo is an excellent model for studying cell migration and axon guidance (Kuwada, 1995;Hutson and Chien, 2002), the potential roles of unconventional myosins in these dynamic cellular processes can be readily investigated.…”

Section: Introductionmentioning

confidence: 99%

Expression of unconventional myosin genes during neuronal development in zebrafish

Sittaramane

Chandrasekhar

2008

Gene Expression Patterns

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Neuronal migration and growth cone motility are essential aspects of the development and maturation of the nervous system. These cellular events result from dynamic changes in the organization and function of the cytoskeleton, in part due to the activity of cytoskeletal motor proteins such as myosins. Although specific myosins such as Myo2 (conventional or muscle myosin), Myo1, and Myo5 have been well characterized for roles in cell motility, the roles of the majority of unconventional (other than Myo2) myosins in cell motility events have not been investigated. To address this issue, we have undertaken an analysis of unconventional myosins in zebrafish, a premier model for studying cellular and growth cone motility in the vertebrate nervous system. We describe the characterization and expression patterns of several members of the unconventional myosin gene family. Based on available genomic sequence data, we identified 18 unconventional myosin- and 4 Myo2-related genes in the zebrafish genome in addition to previously characterized myosin (1, 2, 3, 5, 6, 7) genes. Phylogenetic analyses indicate that these genes can be grouped into existing classifications for unconventional myosins from mouse and man. In situ hybridization analyses using EST probes for 18 of the 22 identified genes indicate that 11/18 genes are expressed in a restricted fashion in the zebrafish embryo. Specific myosins are expressed in particular neuronal or neuroepithelial cell types in the developing zebrafish nervous system, spanning the periods of neuronal differentiation and migration, and of growth cone guidance and motility.

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Myosin VI and VIIa distribution among inner ear epithelia in diverse fishes

Cited by 23 publications

References 68 publications

Notch Signaling Regulates the Extent of Hair Cell Regeneration in the Zebrafish Lateral Line

Notch Signaling Regulates the Extent of Hair Cell Regeneration in the Zebrafish Lateral Line

Phenotypic and Expression Analysis of a Novel Spontaneous Myosin VI Null Mutant Mouse

Expression of unconventional myosin genes during neuronal development in zebrafish

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